Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,996,183	T→C	N217S (AAC→AGC)	sdiA ←	DNA‑binding transcriptional dual regulator SdiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,996,183	0	T	C	100.0%	104.5 / NA	29	N217S (AAC→AGC)	sdiA	DNA‑binding transcriptional dual regulator SdiA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/11); total (18/11)

CCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACT  >  NC_000913/1996050‑1996317

ccGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCt                                                                                                                                  >  2:739227/1‑140 (MQ=255)

   gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggt                                                                                                                               >  2:713829/1‑140 (MQ=255)

             tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                     >  2:329973/1‑140 (MQ=255)

              gCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAcc                                                                                                                    <  1:1233081/140‑1 (MQ=255)

              gCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAcc                                                                                                                    >  2:1233081/1‑140 (MQ=255)

                 tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTCAGCCAGTAGCGGCCGCGTAACAGGCAAGCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                                 <  2:1103207/140‑1 (MQ=255)

                 tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                                 >  2:1064826/1‑140 (MQ=255)

                           tttttGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAgaga                                                                                                       <  1:867868/140‑1 (MQ=255)

                                      ggCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAatc                                                                                            <  1:1064826/140‑1 (MQ=255)

                                                        gagaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGAt                                                                          <  1:739227/140‑1 (MQ=255)

                                                         agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATg                                                                         >  1:334511/1‑140 (MQ=255)

                                                          gaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGt                                                                        >  2:87103/1‑140 (MQ=255)

                                                           aTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGtt                                                                       <  2:404270/140‑1 (MQ=255)

                                                           aTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGtt                                                                       >  2:35915/1‑140 (MQ=255)

                                                           aTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGtt                                                                       >  2:181211/1‑140 (MQ=255)

                                                              aaaTTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTc                                                                    >  1:469413/1‑140 (MQ=255)

                                                                                                 tGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTcgcg                                 <  1:1116218/140‑1 (MQ=255)

                                                                                                   ggTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCt                               <  1:181211/140‑1 (MQ=255)

                                                                                                    gTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCtt                              >  1:488963/1‑140 (MQ=255)

                                                                                                             ggTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAAtt                     <  2:334511/140‑1 (MQ=255)

                                                                                                               tGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAATTTCa                   >  2:1192361/1‑140 (MQ=255)

                                                                                                               tGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCa                   >  1:452517/1‑140 (MQ=255)

                                                                                                               tGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCa                   >  2:687117/1‑140 (MQ=255)

                                                                                                                 cATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCGGATGTTTTCACTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCAtc                 >  2:1103943/1‑140 (MQ=255)

                                                                                                                   ttAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCAtctc               >  1:532400/1‑140 (MQ=255)

                                                                                                                         tttttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGt         <  1:87103/140‑1 (MQ=255)

                                                                                                                             tCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcatc     <  1:713829/140‑1 (MQ=255)

                                                                                                                                gCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACt  >  2:170673/1‑140 (MQ=255)

                                                                                                                                gCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACt  >  1:1169827/1‑140 (MQ=255)

CCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACT  >  NC_000913/1996050‑1996317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A