Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 126 72.5 2472046 99.4% 2457213 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,134,525 A→G N132S (AAC→AGC)  yghT → putative ATP‑binding protein YghT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,134,5250AG100.0% 200.1 / NA 55N132S (AAC→AGC) yghTputative ATP‑binding protein YghT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

AGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAACC  >  NC_000913/3134391‑3134657
                                                                                                                                      |                                                                                                                                    
aGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTg                                                                                                                                 >  1:870879/1‑140 (MQ=255)
      tGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAggtg                                                                                                                           <  2:870879/140‑1 (MQ=255)
      tGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAggtg                                                                                                                           >  2:250270/1‑140 (MQ=255)
       gTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAggtgg                                                                                                                          <  2:1139668/140‑1 (MQ=255)
            ccGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGtt                                                                                                                     <  1:107231/140‑1 (MQ=255)
                     aCTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAt                                                                                                            >  2:282131/1‑140 (MQ=255)
                        gcgcTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGtt                                                                                                                     <  1:180753/128‑1 (MQ=255)
                        gcgcTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGtt                                                                                                                     >  2:180753/1‑128 (MQ=255)
                         cgcTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCGACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggct                                                                                                        >  2:190986/1‑140 (MQ=255)
                         cgcTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggct                                                                                                        >  2:333834/1‑140 (MQ=255)
                           cTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      <  2:896136/140‑1 (MQ=255)
                            tttCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctggc                                                                                                     <  1:250270/140‑1 (MQ=255)
                                   atatGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAg                                                                                              <  2:889956/140‑1 (MQ=255)
                                     atGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCAACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGa                                                                                            >  2:729431/1‑140 (MQ=255)
                                        cTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGCCCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCC‑CCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      >  2:663192/1‑126 (MQ=255)
                                        cTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCC‑CCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      <  1:663192/126‑1 (MQ=255)
                                          tGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTa                                                                                       >  2:376712/1‑140 (MQ=255)
                                            ccTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      >  1:446752/1‑123 (MQ=255)
                                            ccTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCAtggctgg                                                                                                      <  2:446752/123‑1 (MQ=255)
                                              tGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGc                                                                                   >  2:1026141/1‑140 (MQ=255)
                                                tcgtcgtcgCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGt                                                                                 >  2:806867/1‑140 (MQ=255)
                                                 cgtcgtcgCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCg                                                                                  <  1:305170/138‑1 (MQ=255)
                                                 cgtcgtcgCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCg                                                                                  >  2:305170/1‑138 (MQ=255)
                                                  gtcgtcgCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGttt                                                                               <  1:876686/140‑1 (MQ=255)
                                                       cgcgGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTg                                                                          >  2:200189/1‑140 (MQ=255)
                                                         cgGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGa                                                                        <  2:822218/140‑1 (MQ=255)
                                                          gggTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGAt                                                                       <  1:376712/140‑1 (MQ=255)
                                                              tAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCg                                                                   <  2:170863/140‑1 (MQ=255)
                                                              tAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCg                                                                   <  1:200189/140‑1 (MQ=255)
                                                                 ttGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGc                                                                >  2:798476/1‑140 (MQ=255)
                                                                   gTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCa                                                              <  1:1172316/140‑1 (MQ=255)
                                                                   gTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCAACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCa                                                              <  1:729431/140‑1 (MQ=255)
                                                                     tCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATa                                                            >  2:826409/1‑140 (MQ=255)
                                                                      cTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATaa                                                           >  2:357698/1‑140 (MQ=255)
                                                                         accgaccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                                                        >  2:354244/1‑140 (MQ=255)
                                                                         accgaccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                                                        >  2:1037037/1‑140 (MQ=255)
                                                                              ccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCa                                                              >  2:836614/1‑129 (MQ=255)
                                                                              ccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCa                                                              <  1:836614/129‑1 (MQ=255)
                                                                                      cTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGtcatc                                           <  1:826409/140‑1 (MQ=255)
                                                                                                aaTTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATg                                 >  1:1042480/1‑140 (MQ=255)
                                                                                                  ttCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGtt                               <  1:1037037/140‑1 (MQ=255)
                                                                                                      tGGCGCTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGAcc                           <  2:317682/140‑1 (MQ=255)
                                                                                                           cTTACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGaa                      >  2:420357/1‑140 (MQ=255)
                                                                                                            ttACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAg                     <  1:1026141/140‑1 (MQ=255)
                                                                                                             tACGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGt                    >  2:538478/1‑140 (MQ=255)
                                                                                                              aCGATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGtt                   <  1:798476/140‑1 (MQ=255)
                                                                                                                gATGGTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGc                 <  1:357698/140‑1 (MQ=255)
                                                                                                                    gTACGGTGTTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCtgt             <  1:333834/140‑1 (MQ=255)
                                                                                                                          tgtTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGt       >  1:1082967/1‑140 (MQ=255)
                                                                                                                           gtTCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTa      <  2:1042480/140‑1 (MQ=255)
                                                                                                                            ttCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGc                 >  2:1178444/1‑128 (MQ=255)
                                                                                                                            ttCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGc                 <  1:1178444/128‑1 (MQ=255)
                                                                                                                            ttCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTaa     >  1:557222/1‑140 (MQ=255)
                                                                                                                            ttCCCACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTaa     >  1:1181843/1‑140 (MQ=255)
                                                                                                                              cccACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAAc   >  2:650227/1‑140 (MQ=255)
                                                                                                                              cccACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAAc   >  1:400686/1‑140 (MQ=255)
                                                                                                                               ccACCTAGCGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAAcc  <  1:806867/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
AGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAATGTTGACCTTGAAGTTGCCTGTGCACGTAAACC  >  NC_000913/3134391‑3134657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A