Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,636,286	(C)_6→7	coding (1125/1203 nt)	yhiN ←	putative oxidoreductase YhiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,280	1	.	C	98.5%	236.3 / ‑3.6	65	coding (1131/1203 nt)	yhiN	putative oxidoreductase YhiN
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (26/38); total (27/38)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAACCACTATCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑G‑CCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCG  >  NC_000913/3636141‑3636408

gATAACCACTATCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑g                                                                                                                                   <  1:413760/140‑1 (MQ=255)

      cACTATCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑G‑cccccc                                                                                                                            <  2:1037720/140‑1 (MQ=255)

         tATCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGCCCACGCCCACTGGAAGTTATA‑C‑cccccccag                                                                                                                         <  2:1168241/140‑4 (MQ=255)

         tATCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑G‑cccccccag                                                                                                                         >  1:761507/1‑137 (MQ=255)

          aTCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑G‑cccccccagc                                                                                                                        >  2:733476/1‑136 (MQ=255)

          aTCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑G‑cccccccagc                                                                                                                        >  1:487463/1‑136 (MQ=255)

           tCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑G‑cccccccagcc                                                                                                                       >  1:505784/1‑135 (MQ=255)

              aaaTTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCcagccagc                                                                                                                     <  2:470646/140‑1 (MQ=255)

                   aGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTg                                                                                                                <  1:805620/140‑1 (MQ=255)

                    gCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGa                                                                                                               <  1:413298/140‑1 (MQ=255)

                     cTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTg                                                                                                                <  2:739084/138‑1 (MQ=255)

                     cTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTg                                                                                                                >  1:739084/1‑138 (MQ=255)

                     cTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGAc                                                                                                              >  1:1120780/1‑140 (MQ=255)

                     cTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGAc                                                                                                              >  2:301200/1‑140 (MQ=255)

                        aTTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTc                                                                                                           <  1:45105/140‑1 (MQ=255)

                              aaTGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCAc                                                                                                     >  2:646097/1‑140 (MQ=255)

                                   gACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGc                                                                                                <  1:633252/140‑1 (MQ=255)

                                   gACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGc                                                                                                <  1:274551/140‑1 (MQ=255)

                                    aCTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGcc                                                                                               >  1:915557/1‑140 (MQ=255)

                                       ctAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGAt                                                                                            <  1:703865/140‑1 (MQ=255)

                                        taataaTTTTCATCTTTAGGAAATAGGTCAGGACGGCTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATg                                                                                           <  2:841687/140‑1 (MQ=255)

                                        taataaTTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATg                                                                                           <  1:646097/140‑1 (MQ=255)

                                        taataaTTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATg                                                                                           <  1:904819/140‑1 (MQ=255)

                                        taataaTTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATg                                                                                           <  1:504965/140‑1 (MQ=255)

                                             atTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTa                                                                                      <  1:301200/140‑1 (MQ=255)

                                              ttttCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTAc                                                                                     <  1:999660/140‑1 (MQ=255)

                                              ttttCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTAc                                                                                     <  1:206676/140‑1 (MQ=255)

                                                ttCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAg                                                                                   >  2:631859/1‑140 (MQ=255)

                                                             aaTAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTg                                                                      <  2:1119077/140‑1 (MQ=255)

                                                               tAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTgcg                                                                    <  2:802850/140‑1 (MQ=255)

                                                                aGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTgcgc                                                                   >  2:182430/1‑140 (MQ=255)

                                                                  gTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTgcgcgc                                                                 >  1:3036/1‑140 (MQ=255)

                                                                  gTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTgcgcgc                                                                 >  2:627451/1‑140 (MQ=255)

                                                                   tCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCt                                                                >  1:508320/1‑140 (MQ=255)

                                                                      ggACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTcc                                                             >  2:536429/1‑140 (MQ=255)

                                                                      ggACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTcc                                                             <  2:1127960/140‑1 (MQ=255)

                                                                       gacgacTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCa                                                            >  2:780045/1‑140 (MQ=255)

                                                                       gacgacTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCa                                                            >  2:484975/1‑140 (MQ=255)

                                                                        acgacTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCAt                                                           <  1:780045/140‑1 (MQ=255)

                                                                          gacTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCg                                                         <  1:627451/140‑1 (MQ=255)

                                                                          gacTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCg                                                         <  1:536429/140‑1 (MQ=255)

                                                                          gacTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCg                                                         <  1:484975/140‑1 (MQ=255)

                                                                            cTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCAt                                                           >  2:960530/1‑136 (MQ=255)

                                                                            cTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCAt                                                           <  1:960530/136‑1 (MQ=255)

                                                                            cTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTc                                                       >  1:713982/1‑140 (MQ=255)

                                                                             tttGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTcc                                                      <  2:1120780/140‑1 (MQ=255)

                                                                             tttGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTcc                                                      <  2:487463/140‑1 (MQ=255)

                                                                              ttGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCGCGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCg                                                     <  1:981401/140‑1 (MQ=255)

                                                                                      aTCAAATCCTGCGCACAAGCCCACGCACTCGGCCACGCCCACCGGAAGTTATACC‑CCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGCACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAagaga                                             >  2:29014/1‑140 (MQ=255)

                                                                                      aTCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAagaga                                             >  2:727508/1‑140 (MQ=255)

                                                                                      aTCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAagaga                                             >  2:638078/1‑140 (MQ=255)

                                                                                      aTCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAagaga                                             <  1:182430/140‑1 (MQ=255)

                                                                                         aaaTCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGtt                                          <  1:733476/140‑1 (MQ=255)

                                                                                              cTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTg                                     <  2:939999/140‑1 (MQ=255)

                                                                                                gcgcACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCAGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTggg                                   >  1:534333/1‑139 (MQ=255)

                                                                                                 cgcACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGgtg                                  >  1:257452/1‑140 (MQ=255)

                                                                                                 cgcACAAGCCCACGCACTCGACCACGCCCACTGGAAGATATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGgtg                                  >  1:758404/1‑140 (MQ=255)

                                                                                                      aaGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCAc                             >  1:30095/1‑140 (MQ=255)

                                                                                                                aCTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGc                   <  2:915557/140‑1 (MQ=255)

                                                                                                                aCTCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGc                   <  2:3036/140‑1 (MQ=255)

                                                                                                                  tCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                 <  1:727508/140‑1 (MQ=255)

                                                                                                                  tCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                 <  2:508320/140‑1 (MQ=255)

                                                                                                                  tCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                 <  1:638078/140‑1 (MQ=255)

                                                                                                                  tCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                 >  1:968249/1‑140 (MQ=255)

                                                                                                                  tCGACCACGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                 <  2:1085323/140‑1 (MQ=255)

                                                                                                                        aCGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTc           <  2:1043268/140‑1 (MQ=255)

                                                                                                                         cGCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCg          >  1:159076/1‑140 (MQ=255)

                                                                                                                          gCCCACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCgg         >  2:631119/1‑140 (MQ=255)

                                                                                                                            ccACTGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCa       >  1:1156059/1‑140 (MQ=255)

                                                                                                                                tGGAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGc   >  2:1191624/1‑140 (MQ=255)

                                                                                                                                 ggAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCg  <  1:631119/140‑1 (MQ=255)

                                                                                                                                 ggAAGTTATA‑GCCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCg  <  1:746250/140‑1 (MQ=255)

GATAACCACTATCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATA‑G‑CCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCG  >  NC_000913/3636141‑3636408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A