Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	453,781	G→A	I129I (ATC→ATT)	yajG ←	putative lipoprotein YajG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	453,781	0	G	A	100.0%	130.4 / NA	37	I129I (ATC→ATT)	yajG	putative lipoprotein YajG
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/18); total (19/18)

GTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGT  >  NC_000913/453650‑453910

gTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGc                                                                                                                           >  1:630849/1‑140 (MQ=255)

        cAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTcg                                                                                                                   >  1:228682/1‑140 (MQ=255)

         aGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTcgc                                                                                                                  <  1:816470/140‑1 (MQ=255)

                tGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTcg                                                                                                                   <  2:272715/132‑1 (MQ=255)

                tGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTcg                                                                                                                   >  1:272715/1‑132 (MQ=255)

                tGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGAtgttg                                                                                                           >  1:631588/1‑140 (MQ=255)

                   tAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAg                                                                                                        <  2:630849/140‑1 (MQ=255)

                         cATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACg                                                                                                  <  2:527584/140‑1 (MQ=255)

                         cATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACg                                                                                                  <  2:785717/140‑1 (MQ=255)

                          aTCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGt                                                                                                 <  2:809904/140‑1 (MQ=255)

                          aTCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGt                                                                                                 >  2:199593/1‑140 (MQ=255)

                          aTCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGt                                                                                                 >  1:377761/1‑140 (MQ=255)

                            cGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTg                                                                                               >  2:598905/1‑140 (MQ=255)

                                      tttGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGaca                                                                                     <  1:238608/140‑1 (MQ=255)

                                            ggAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAg                                                                               <  1:287143/140‑1 (MQ=255)

                                             gAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGc                                                                              <  2:799016/140‑1 (MQ=255)

                                                        cGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGc                                                                   <  1:77754/140‑1 (MQ=255)

                                                          cACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTa                                                                 >  2:672915/1‑140 (MQ=255)

                                                          cACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTa                                                                 >  1:795893/1‑140 (MQ=255)

                                                          cACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTa                                                                 >  2:467791/1‑140 (MQ=255)

                                                           aCCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCa                                                                             >  1:344583/1‑127 (MQ=255)

                                                           aCCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCa                                                                             <  2:344583/127‑1 (MQ=255)

                                                                   cGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGAtt                                                        <  1:467791/140‑1 (MQ=255)

                                                                       gTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCa                                                    >  1:131205/1‑140 (MQ=255)

                                                                       gTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCa                                                    >  1:540132/1‑140 (MQ=255)

                                                                       gTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCa                                                    >  1:450777/1‑140 (MQ=255)

                                                                             ggCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTaa                                              >  1:708721/1‑140 (MQ=255)

                                                                                aCGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCg                                           >  2:426592/1‑140 (MQ=255)

                                                                                  gATACTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCggg                                         <  2:795893/140‑1 (MQ=255)

                                                                                             gTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              <  2:377761/140‑1 (MQ=255)

                                                                                             gTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              <  2:228682/140‑1 (MQ=255)

                                                                                             gTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCAATATCCACTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              <  1:121423/140‑1 (MQ=255)

                                                                                                            ttCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              <  2:549983/125‑1 (MQ=255)

                                                                                                            ttCTGCGCGGTAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGacc                              >  1:549983/1‑125 (MQ=255)

                                                                                                                      tAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATc     >  2:343483/1‑140 (MQ=255)

                                                                                                                      tAGCGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGAAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATc     >  2:167040/1‑140 (MQ=255)

                                                                                                                         cGATGATGGCAATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGt  <  2:631588/140‑1 (MQ=255)

GTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGT  >  NC_000913/453650‑453910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A