Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,388,183	A→G	K488K (AAA→AAG)	tyrR →	DNA‑binding transcriptional dual regulator TyrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,388,183	0	A	G	100.0%	125.0 / NA	35	K488K (AAA→AAG)	tyrR	DNA‑binding transcriptional dual regulator TyrR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/20); total (15/20)

GATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGG  >  NC_000913/1388049‑1388295

gATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGtct                                                                                                             <  1:81826/140‑1 (MQ=255)

  ttATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCg                                                                                                           <  2:121312/140‑1 (MQ=255)

  ttATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCg                                                                                                           <  2:452893/140‑1 (MQ=255)

    aTGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGc                                                                                                         <  1:384964/140‑1 (MQ=255)

        cgccgcAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGttt                                                                                                     <  2:555407/140‑1 (MQ=255)

           cgcAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTcac                                                                                                  >  2:615364/1‑140 (MQ=255)

              aaCGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATa                                                                                               >  2:307359/1‑140 (MQ=255)

                         tGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGcc                                                                                    >  1:161112/1‑140 (MQ=255)

                           ggCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCaa                                                                                  >  2:329830/1‑140 (MQ=255)

                            gCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAAt                                                                                 <  2:802962/140‑1 (MQ=255)

                                    gCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCg                                                                         <  1:311811/140‑1 (MQ=255)

                                          gAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAata                                                                   >  2:284490/1‑140 (MQ=255)

                                             ggTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATgg                                                                <  1:615364/140‑1 (MQ=255)

                                             ggTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATgg                                                                <  2:296387/140‑1 (MQ=255)

                                                    tGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGt                                                         <  2:419559/140‑1 (MQ=255)

                                                      gACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCa                                                       >  1:679207/1‑140 (MQ=255)

                                                       aCGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCag                                                      <  1:329830/140‑1 (MQ=255)

                                                        cGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAata                                                                   >  1:379893/1‑126 (MQ=255)

                                                        cGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAata                                                                   <  2:379893/126‑1 (MQ=255)

                                                                ccAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCt                                                             >  1:720522/1‑124 (MQ=255)

                                                                ccAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCt                                                             <  2:720522/124‑1 (MQ=255)

                                                                         ttGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTaa                                    >  2:286141/1‑140 (MQ=255)

                                                                           gAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAgc                                  <  1:286141/140‑1 (MQ=255)

                                                                                         aaCCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGa                    <  1:284490/140‑1 (MQ=255)

                                                                                           cccAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATg                  >  1:206551/1‑140 (MQ=255)

                                                                                              aGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTg               <  2:161112/140‑1 (MQ=255)

                                                                                               gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCGCATACCGCGATTGCCTATAAGTTGCGTGAATATGGTCTAAGTCAGAAGAAGATCGAAGAGTAAGGGCGAATATGCCTGATGGTGc              >  2:511787/1‑140 (MQ=255)

                                                                                               gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGt                >  2:491789/1‑138 (MQ=255)

                                                                                               gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGt                <  1:491789/138‑1 (MQ=255)

                                                                                               gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGc              >  2:317555/1‑140 (MQ=255)

                                                                                               gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGc              >  2:410200/1‑140 (MQ=255)

                                                                                               gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGc              >  2:533792/1‑140 (MQ=255)

                                                                                               gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGc              >  2:582350/1‑140 (MQ=255)

                                                                                                     tCGCAATTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACAcc        <  2:308196/140‑1 (MQ=255)

                                                                                                          aTTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg   <  1:511787/140‑1 (MQ=255)

                                                                                                          aTTATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg   <  2:679207/140‑1 (MQ=255)

                                                                                                           ttATCCCAGCACGCGCAAACTGGCAAAGCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAgg  <  2:206551/140‑1 (MQ=255)

GATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGG  >  NC_000913/1388049‑1388295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A