Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,565,109	G→A	Q209* (CAG→TAG)	dosP ←	oxygen‑sensing c‑di‑GMP phosphodiesterase DosP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,565,109	0	G	A	100.0%	120.6 / NA	35	Q209* (CAG→TAG)	dosP	oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/18); total (17/18)

CGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAATGCG  >  NC_000913/1564979‑1565245

cGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCgcgggc                                                                                                                                 >  1:534698/1‑140 (MQ=255)

            atatCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTC‑ACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGc                                                                                                                    <  2:169508/140‑1 (MQ=255)

               tCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCaa                                                                                                                  >  2:670162/1‑140 (MQ=255)

                        gTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTaa                                                                                                         >  1:584918/1‑140 (MQ=255)

                               ccAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATg                                                                                                  <  1:670162/140‑1 (MQ=255)

                                       tGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATc                                                                                          >  1:754993/1‑140 (MQ=255)

                                       tGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATc                                                                                          >  1:44444/1‑140 (MQ=255)

                                       tGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATc                                                                                          >  1:56764/1‑140 (MQ=255)

                                           gATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGTCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCa                                                                                      <  2:739572/140‑1 (MQ=255)

                                           gATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCa                                                                                      <  2:754993/140‑1 (MQ=255)

                                             tGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGTGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAgg                                                                                    >  2:214050/1‑140 (MQ=255)

                                              gcgcGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAggg                                                                                   >  1:1065/1‑140 (MQ=255)

                                              gcgcGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAggg                                                                                   >  1:860601/1‑140 (MQ=255)

                                                 cgAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAAt                                                                                >  1:487673/1‑140 (MQ=255)

                                                 cgAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAAt                                                                                >  1:441568/1‑140 (MQ=255)

                                                     cACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAg                                                                            <  1:293790/140‑1 (MQ=255)

                                                       cGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGa                                                                          <  2:487673/140‑1 (MQ=255)

                                                        gTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGa                                                                          >  1:605573/1‑139 (MQ=255)

                                                        gTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGa                                                                          <  2:605573/139‑1 (MQ=255)

                                                            tAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGtt                                                                     <  2:56764/140‑1 (MQ=255)

                                                                   ggCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAgt                                                              >  1:474029/1‑140 (MQ=255)

                                                                           gagGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCggg                                                      <  1:295468/140‑1 (MQ=255)

                                                                           gagGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCggg                                                      <  2:474029/140‑1 (MQ=255)

                                                                              gCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTg                                                   >  1:363829/1‑140 (MQ=255)

                                                                                tttAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGTGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCa                                                 <  1:214050/140‑1 (MQ=255)

                                                                                  tAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATa                                               <  2:363829/140‑1 (MQ=255)

                                                                                     tCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCg                                            <  1:302773/140‑1 (MQ=255)

                                                                                        aGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTg                                         >  2:501406/1‑140 (MQ=255)

                                                                                         gATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCttg                                        <  2:534698/140‑3 (MQ=255)

                                                                                         gATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTgg                                        >  1:529934/1‑140 (MQ=255)

                                                                                            tttttCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCtt                                     <  2:441568/140‑1 (MQ=255)

                                                                                                                    cagAAATTCGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACAttt             <  2:529934/140‑1 (MQ=255)

                                                                                                                            cGTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAAt     >  2:255385/1‑140 (MQ=255)

                                                                                                                             gTCCTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAATg    <  2:584918/140‑1 (MQ=255)

                                                                                                                               ccTAATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAATgcg  <  1:501406/140‑1 (MQ=255)

CGTTCTTCGGTGATATCCGAGAAAGTCATTACCAGGTTCTGCAGATGCGCGAGCACGTCATAAACCGGGCTGATAGAGGCTTTAATCCAGATTTTTTCACCGGTGCGCGTCAACAGCAGAAATTCGTCCTGATCGCGGGCGGTTTTCCATAGCAACTGTTGTAAACGAATGCGGTTATCGGCAGGGAATTCAGGAATGTTCAGGAGTGTATCGGGCTGCATACCGCTGGCTTCGCTAATGCAGTAACCAAACATTTCGGTAAATGCG  >  NC_000913/1564979‑1565245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A