Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,205,386	T→C	V264A (GTC→GCC)	yehL →	putative AAA(+) MoxR family ATPase YehL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,205,386	0	T	C	100.0%	121.6 / NA	34	V264A (GTC→GCC)	yehL	putative AAA(+) MoxR family ATPase YehL
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/16); total (18/16)

CTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGTCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATCGC  >  NC_000913/2205252‑2205522

cTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCa                                                                                                                                     <  2:44755/140‑1 (MQ=255)

 ttCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCAc                                                                                                                                    <  1:154565/140‑1 (MQ=255)

     aTTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCAc                                                                                                                                    >  2:660697/1‑136 (MQ=255)

     aTTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCAc                                                                                                                                    <  1:660697/136‑1 (MQ=255)

         tGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCcgcg                                                                                                                            <  2:836472/140‑1 (MQ=255)

                     tCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCa                                                                                                                >  2:659999/1‑140 (MQ=255)

                               gATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCg                                                                                                          >  1:699340/1‑136 (MQ=255)

                               gATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCg                                                                                                          <  2:699340/136‑1 (MQ=255)

                                  ttCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCgaaaagaa                                                                                                   <  2:503140/140‑1 (MQ=255)

                                    cGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCgaaaagaaaa                                                                                                 >  2:821875/1‑140 (MQ=255)

                                        cAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTc                                                                                             <  1:821875/140‑1 (MQ=255)

                                          ggAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCaa                                                                                           <  1:391599/140‑1 (MQ=255)

                                            ggTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATg                                                                                         <  1:601533/139‑1 (MQ=255)

                                                       gTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGAc                                                                              >  1:840886/1‑140 (MQ=255)

                                                       gTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGAc                                                                              >  2:166394/1‑140 (MQ=255)

                                                              gTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGAtt                                                                       <  1:210041/140‑1 (MQ=255)

                                                                 cTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGGGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATg                                                                    >  2:623894/1‑140 (MQ=255)

                                                                 cTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATg                                                                    >  2:763192/1‑140 (MQ=255)

                                                                 cTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATg                                                                    >  2:474231/1‑140 (MQ=255)

                                                                 cTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATg                                                                    >  2:562253/1‑140 (MQ=255)

                                                                         cgTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGc                                                            >  2:721000/1‑140 (MQ=255)

                                                                                        aGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGt                                             <  1:166394/140‑1 (MQ=255)

                                                                                        aGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGt                                             <  1:659999/140‑1 (MQ=255)

                                                                                            gTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGcc                                         >  2:586987/1‑140 (MQ=255)

                                                                                            gTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGcc                                         >  1:554965/1‑140 (MQ=255)

                                                                                               ttCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCAt                                      >  1:323301/1‑140 (MQ=255)

                                                                                                tCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATg                                     <  2:60680/140‑1 (MQ=255)

                                                                                                  cGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCt                                   >  1:668578/1‑140 (MQ=255)

                                                                                                                gACGCGGTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCgcgc                     <  2:486263/140‑1 (MQ=255)

                                                                                                                     ggTACTGGAATTACTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGt                >  1:795156/1‑140 (MQ=255)

                                                                                                                                 aCTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATc    >  2:324787/1‑140 (MQ=255)

                                                                                                                                 aCTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATc    >  1:681950/1‑140 (MQ=255)

                                                                                                                                  cTGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATcg   <  1:586987/140‑1 (MQ=255)

                                                                                                                                   tGGCCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATcgc  <  2:668578/140‑1 (MQ=255)

CTTCGATTTTGAAACGGTTTTTCCGATTATGGATTTCGCCCAGGAGTTGGAACTGGTCGCCAGTGCTTCGGCGCGTTTGCTGGCCCATAGCGGTATTCCGCATAAAGTGCCTGACGCGGTACTGGAATTACTGGTCCGCACCTTCCGCGATCTGCGTGCCAACGGCGAAAAGAAAACGTCAATGGATACACTGACAGCGATTATGTCCACCGCTGAAGCCGTGAACGTGGCCCATGCTGTGGGCGTCCGCGCCTGGTTTTTAGCGAATCGC  >  NC_000913/2205252‑2205522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A