Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
123 |
51.0 |
1731148 |
99.3% |
1719029 |
138.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,634,926 |
T→C |
V232A (GTT→GCT) |
xseA → |
exodeoxyribonuclease VII subunit XseA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,634,926 | 0 | T | C | 100.0%
| 81.4
/ NA
| 24 | V232A (GTT→GCT) | xseA | exodeoxyribonuclease VII subunit XseA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/11); total (13/11) |
TGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGC > NC_000913/2634803‑2635054
|
tGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCggg < 1:97189/140‑1 (MQ=255)
gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa > 2:846522/1‑140 (MQ=255)
gCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCa > 2:60066/1‑140 (MQ=255)
tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga > 1:420346/1‑140 (MQ=255)
tCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATgaga < 2:73644/140‑1 (MQ=255)
tGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGa > 2:346512/1‑140 (MQ=255)
gATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTttg < 1:346512/140‑1 (MQ=255)
aTCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTttgt > 1:731928/1‑140 (MQ=255)
tcgtcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTttgtt < 1:60066/140‑1 (MQ=255)
cgtcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTttgttg < 2:836898/140‑1 (MQ=255)
gtcgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGc > 2:548594/1‑140 (MQ=255)
cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg > 1:651743/1‑140 (MQ=255)
cgGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTCAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCg > 1:333910/1‑140 (MQ=255)
ggCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGAt > 1:753802/1‑140 (MQ=255)
aTTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTgcc < 1:73944/140‑1 (MQ=255)
aGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGa > 1:399316/1‑140 (MQ=255)
tAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAagtag > 1:167753/1‑140 (MQ=255)
aCGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTa < 2:280898/140‑1 (MQ=255)
aCGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTa < 1:548594/140‑1 (MQ=255)
aGCGCGGGCGATTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGc < 2:731928/140‑1 (MQ=255)
aTTTTTACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACt < 1:846522/140‑1 (MQ=255)
ttttACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTgc > 2:793455/1‑140 (MQ=255)
tACCAGCCGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGcc < 2:420346/140‑1 (MQ=255)
ccGCATTCCGGCTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGc > 1:79323/1‑140 (MQ=255)
|
TGGCGAATCAGCGCAATGAGTGCGACGTATTGATCGTCGGGCGCGGCGGCGGTTCGCTGGAAGATTTATGGAGTTTTAACGACGAACGCGTAGCGCGGGCGATTTTTACCAGCCGCATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGC > NC_000913/2634803‑2635054
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
