Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,703,198	T→C	T63T (ACA→ACG)	era ←	30S ribosomal subunit maturation GTPase Era

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,703,198	0	T	C	100.0%	112.0 / NA	31	T63T (ACA→ACG)	era	30S ribosomal subunit maturation GTPase Era
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/15); total (16/15)

GCACCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGT  >  NC_000913/2703062‑2703334

gCACCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTa                                                                                                                                       <  1:323624/140‑1 (MQ=255)

 cACCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTAt                                                                                                                                      <  1:93447/140‑1 (MQ=255)

  aCCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTa                                                                                                                                       >  1:587474/1‑138 (MQ=255)

  aCCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTa                                                                                                                                       <  2:587474/138‑1 (MQ=255)

  aCCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATc                                                                                                                                     >  1:496720/1‑140 (MQ=255)

      tttCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACg                                                                                                                                 >  1:190964/1‑140 (MQ=255)

         cgtcgtCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAg                                                                                                                              >  2:107422/1‑140 (MQ=255)

          gtcgtcCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGa                                                                                                                             <  2:190964/140‑1 (MQ=255)

           tcgtcCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGAt                                                                                                                            <  1:588088/140‑1 (MQ=255)

              tcCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGc                                                                                                                         >  1:643196/1‑140 (MQ=255)

                          cGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGcc                                                                                                             <  1:360700/140‑1 (MQ=255)

                          cGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGcc                                                                                                             <  1:107422/140‑1 (MQ=255)

                                 cTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTa                                                                                                      <  2:163942/140‑1 (MQ=255)

                                 cTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTACTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTa                                                                                                      <  2:40097/140‑1 (MQ=255)

                                          cAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGcc                                                                                                             >  1:225238/1‑124 (MQ=255)

                                          cAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGcc                                                                                                             <  2:225238/124‑1 (MQ=255)

                                                                   aaTAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTgcg                                                                    <  2:643196/140‑1 (MQ=255)

                                                                     tAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGcc                                                                  <  2:496720/140‑1 (MQ=255)

                                                                          ctgctCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCg                                                             >  2:594648/1‑140 (MQ=255)

                                                                                            atcaGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGAtttt                                           >  1:82585/1‑140 (MQ=255)

                                                                                               aGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTg                                        >  1:390161/1‑140 (MQ=255)

                                                                                                       aaTGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                <  2:390161/140‑1 (MQ=255)

                                                                                                       aaTGGCGCGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                <  1:594648/140‑1 (MQ=255)

                                                                                                           gcgcGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGttt                            >  2:194571/1‑140 (MQ=255)

                                                                                                           gcgcGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGttt                            >  1:763670/1‑140 (MQ=255)

                                                                                                           gcgcGTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGttt                            >  2:690043/1‑140 (MQ=255)

                                                                                                               gTTTTTCTTCCATATGCAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTc                        >  1:223356/1‑140 (MQ=255)

                                                                                                                               cAGGCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGc        <  2:763670/140‑1 (MQ=255)

                                                                                                                                  gCCCGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCaa     >  1:233883/1‑140 (MQ=255)

                                                                                                                                  gCCCGGCGTATCGACGTAGATCACCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCaa     >  2:232691/1‑140 (MQ=255)

                                                                                                                                     cGGCGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGt  >  1:575359/1‑140 (MQ=255)

GCACCATTTCGTCGTCCGGCGTCCAGCGGGTGCCTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGT  >  NC_000913/2703062‑2703334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A