Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,974,973	T→A	I348F (ATC→TTC)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,974,973	0	T	A	100.0%	97.3 / NA	29	I348F (ATC→TTC)	aas	acyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (15/14); total (15/14)

TCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAA  >  NC_000913/2974838‑2975091

tCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCAt                                                                                                                    <  2:13542/140‑1 (MQ=255)

tCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCAt                                                                                                                    <  1:830933/140‑1 (MQ=255)

       aaTGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGt                                                                                                             >  1:312566/1‑140 (MQ=255)

       aaTGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGt                                                                                                             <  1:633091/140‑1 (MQ=255)

          gCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCgg                                                                                                          >  1:641881/1‑140 (MQ=255)

                                ccTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTaa                                                                                    <  1:51034/140‑1 (MQ=255)

                                ccTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTaa                                                                                    <  2:641881/140‑1 (MQ=255)

                                 cTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTaaa                                                                                   >  1:636104/1‑140 (MQ=255)

                                      ggATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTc                                                                              <  2:280157/140‑1 (MQ=255)

                                             cTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAg                                                                       >  2:714803/1‑140 (MQ=255)

                                                   aaCCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAg                                                                 <  2:42736/140‑1 (MQ=255)

                                                        gAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAgcgccc                                                            <  2:636104/140‑3 (MQ=255)

                                                             aaaaaGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAg                                                       >  2:417797/1‑140 (MQ=255)

                                                                           ccTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGc                                         <  2:451377/140‑1 (MQ=255)

                                                                            ctctTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCa                                        <  1:417797/140‑1 (MQ=255)

                                                                             tcttctTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAg                                       >  1:53384/1‑140 (MQ=255)

                                                                                 cttcCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGc                                   <  1:771075/140‑1 (MQ=255)

                                                                                                     tgtgCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCgag               >  1:360542/1‑140 (MQ=255)

                                                                                                      gtgCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCgaga              >  2:71057/1‑140 (MQ=255)

                                                                                                      gtgCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCgaga              >  1:224737/1‑140 (MQ=255)

                                                                                                        gCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGaaa            >  2:266988/1‑140 (MQ=255)

                                                                                                        gCCAGACGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGaaa            <  2:360542/140‑1 (MQ=255)

                                                                                                             aCGCGGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGc       <  2:72885/140‑1 (MQ=255)

                                                                                                             aCGCGGCATCAACAAATGAGCGCAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGc       <  2:388560/140‑1 (MQ=255)

                                                                                                              cgcgGCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCg      >  1:484607/1‑140 (MQ=255)

                                                                                                                 ggCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGa   >  2:285976/1‑140 (MQ=255)

                                                                                                                 ggCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGa   >  1:733401/1‑140 (MQ=255)

                                                                                                                 ggCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGC‑TTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCg      >  2:126032/1‑136 (MQ=255)

                                                                                                                 ggCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGC‑TTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCg      <  1:126032/136‑1 (MQ=255)

                                                                                                                  gCATCAGCAAATGAGCGAAGAACCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGaa  >  1:241689/1‑140 (MQ=255)

TCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGCTCCGGCAGATGCCAGAGTTTGCCTTTATCGAGAAACTGGCGGGAA  >  NC_000913/2974838‑2975091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A