Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F60 I1 R1	16	51.6	1759406	98.6%	1734774	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,005,828	C→A	intergenic (+50/‑124)	pyrD → / → zapC	dihydroorotate dehydrogenase, type 2/cell division protein ZapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,005,828	0	C	A	100.0%	171.7 / NA	50	intergenic (+50/‑124)	pyrD/zapC	dihydroorotate dehydrogenase, type 2/cell division protein ZapC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (28/22); total (28/22)

GCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATG  >  NC_000913/1005692‑1005954

gctgcGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCt                                                                                                                             >  2:854069/1‑140 (MQ=255)

  tgcGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTgg                                                                                                                           >  2:810273/1‑140 (MQ=255)

    cGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGgtt                                                                                                                         <  2:167627/140‑1 (MQ=255)

       gTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGgttgtt                                                                                                                      <  1:649605/140‑1 (MQ=255)

          ccTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTgg                                                                                                                           >  1:792129/1‑132 (MQ=255)

          ccTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTgg                                                                                                                           <  2:792129/132‑1 (MQ=255)

            tCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTtata                                                                                                                 >  2:403921/1‑140 (MQ=255)

              aCTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATAtt                                                                                                               <  1:403921/140‑1 (MQ=255)

                         tttATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTg                                                                                                    <  1:854069/140‑1 (MQ=255)

                            aTTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCtt                                                                                                 >  1:508079/1‑140 (MQ=255)

                                  gTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAg                                                                                           >  1:54316/1‑140 (MQ=255)

                                            tAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGt                                                                                  <  1:591731/139‑1 (MQ=255)

                                            tAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGt                                                                                  >  2:591731/1‑139 (MQ=255)

                                            tAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTa                                                                                 >  1:738822/1‑140 (MQ=255)

                                                  tCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTa                                                                           >  1:472487/1‑140 (MQ=255)

                                                      ccgcTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTAttgt                                                                       >  2:378003/1‑140 (MQ=255)

                                                                aaGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGttatt                                                             <  1:10206/140‑1 (MQ=255)

                                                                      tCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTaagcga                                                       <  2:54316/140‑1 (MQ=255)

                                                                      tCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTaagcga                                                       <  2:738822/140‑1 (MQ=255)

                                                                        gTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTaagcgaag                                                     <  2:450197/140‑1 (MQ=255)

                                                                           aCCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAagcgaagcga                                                  >  2:367180/1‑140 (MQ=255)

                                                                                 atCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATgg                                            >  2:163505/1‑140 (MQ=255)

                                                                                      attattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACa                                                >  2:586556/1‑131 (MQ=255)

                                                                                      attattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACa                                                <  1:74890/131‑1 (MQ=255)

                                                                                      attattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACa                                                <  1:586556/131‑1 (MQ=255)

                                                                                      attattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACa                                                >  2:74890/1‑131 (MQ=255)

                                                                                           ttcttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgc                                  >  2:429471/1‑140 (MQ=255)

                                                                                           ttcttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgc                                  >  1:495591/1‑140 (MQ=255)

                                                                                            tcttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgct                                 >  2:39124/1‑140 (MQ=255)

                                                                                             cttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgctg                                <  1:429471/140‑1 (MQ=255)

                                                                                             cttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgctg                                <  1:810273/140‑1 (MQ=255)

                                                                                                cGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgctgctg                             <  2:524074/140‑3 (MQ=255)

                                                                                                cGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCgg                             <  2:495591/140‑1 (MQ=255)

                                                                                                     tCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTta                        <  1:163505/140‑1 (MQ=255)

                                                                                                                 gATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGga            >  2:349416/1‑140 (MQ=255)

                                                                                                                 gATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGga            >  2:748561/1‑140 (MQ=255)

                                                                                                                    aaTCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGagag         >  1:690339/1‑140 (MQ=255)

                                                                                                                    aaTCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGagag         >  1:642949/1‑140 (MQ=255)

                                                                                                                    aaTCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGagag         >  2:95735/1‑140 (MQ=255)

                                                                                                                      tCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggt                  >  2:215614/1‑129 (MQ=255)

                                                                                                                      tCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggt                  <  1:215614/129‑1 (MQ=255)

                                                                                                                      tCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAgg        <  1:741206/139‑1 (MQ=255)

                                                                                                                      tCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAgg        >  2:741206/1‑139 (MQ=255)

                                                                                                                      tCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGc       >  2:712605/1‑140 (MQ=255)

                                                                                                                          ggCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGga            >  1:743027/1‑131 (MQ=255)

                                                                                                                          ggCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGga            <  2:743027/131‑1 (MQ=255)

                                                                                                                          ggCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGAt   <  1:349416/140‑1 (MQ=255)

                                                                                                                           gCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATg  >  1:530311/1‑140 (MQ=255)

                                                                                                                            cTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggta                 <  2:406700/124‑1 (MQ=255)

                                                                                                                            cTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTAtagggta                 >  1:406700/1‑124 (MQ=255)

GCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATG  >  NC_000913/1005692‑1005954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A