Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F60 I1 R1	18	40.4	1393842	98.9%	1378509	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,005,828	C→A	intergenic (+50/‑124)	pyrD → / → zapC	dihydroorotate dehydrogenase, type 2/cell division protein ZapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,005,828	0	C	A	100.0%	128.7 / NA	37	intergenic (+50/‑124)	pyrD/zapC	dihydroorotate dehydrogenase, type 2/cell division protein ZapC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (21/16); total (21/16)

GGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCGAATTAAAC  >  NC_000913/1005709‑1005964

ggTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCAt                                                                                                                      <  1:271527/140‑1 (MQ=255)

        tttATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTg                                                                                                              <  2:649989/140‑1 (MQ=255)

                 gTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAg                                                                                                     >  1:261288/1‑140 (MQ=255)

                 gTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAg                                                                                                     >  2:304385/1‑140 (MQ=255)

                          ttAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGt                                                                                            <  2:261288/140‑1 (MQ=255)

                            aaaGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTAc                                                                                          >  1:115259/1‑140 (MQ=255)

                            aaaGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTAc                                                                                          <  1:251446/140‑1 (MQ=255)

                            aaaGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTAc                                                                                          >  2:293442/1‑140 (MQ=255)

                            aaaGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTAc                                                                                          <  2:115259/140‑1 (MQ=255)

                               ggTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACttt                                                                                       <  2:379482/140‑1 (MQ=255)

                                 tCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTa                                                                                     <  2:314219/140‑1 (MQ=255)

                                       gcTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTAttgttg                                                                               <  1:293442/140‑1 (MQ=255)

                                                     tCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTaagcga                                                                 >  2:71229/1‑140 (MQ=255)

                                                       gTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTaagcgaag                                                               <  1:118834/140‑1 (MQ=255)

                                                            ccATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACa                                                          <  1:291966/140‑1 (MQ=255)

                                                                atCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATgg                                                      >  2:268051/1‑140 (MQ=255)

                                                                     attattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATtgt                                                 >  1:208865/1‑140 (MQ=255)

                                                                     attattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATtgt                                                 >  1:682498/1‑140 (MQ=255)

                                                                      ttattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATtgtg                                                >  2:98238/1‑140 (MQ=255)

                                                                      ttattTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATtgtg                                                <  1:268051/140‑1 (MQ=255)

                                                                          ttcttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgc                                            >  2:172887/1‑140 (MQ=255)

                                                                          ttcttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgc                                            >  2:567270/1‑140 (MQ=255)

                                                                             ttcGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTtgctgc                                         >  1:339105/1‑140 (MQ=255)

                                                                               cGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCgg                                       <  1:304385/140‑1 (MQ=255)

                                                                                    tCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTta                                  <  2:339105/140‑1 (MQ=255)

                                                                                                 aTAATCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGgag                     >  2:413140/1‑140 (MQ=255)

                                                                                                   aaTCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGagag                   >  2:251444/1‑140 (MQ=255)

                                                                                                   aaTCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGagag                   >  1:137143/1‑140 (MQ=255)

                                                                                                   aaTCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGagag                   >  2:268062/1‑140 (MQ=255)

                                                                                                     tCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGc                 >  2:200640/1‑140 (MQ=255)

                                                                                                     tCAGGGCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGc                 >  1:416045/1‑140 (MQ=255)

                                                                                                         ggCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGAt             <  1:251444/140‑1 (MQ=255)

                                                                                                          gCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATg            >  1:215233/1‑140 (MQ=255)

                                                                                                          gCTTTATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATg            <  2:682498/140‑1 (MQ=255)

                                                                                                              tATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCGaa        >  2:487406/1‑140 (MQ=255)

                                                                                                              tATTTTCAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCGaa        >  1:526086/1‑140 (MQ=255)

                                                                                                                    cAGACCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCGAATTAAAc  <  1:98238/140‑1 (MQ=255)

GGTGCAAATTTATTCTGGTTTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAATTATTTCTTCGAATTCCTCTTTATAAGATAATCAGGGCTTTATTTTCAGCCCTGGTTGTTTTATATTCATCGCTGTTGCTTATTTAGACATTTTGTACTTTTATTGTTGAGGTTATTAAGCGAAGCGACAATGGATTGTGTTGCTGCGGTTTTATAGGGTAGGGGAGAGGCAGATGCGAATTAAAC  >  NC_000913/1005709‑1005964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A