Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	143	68.4	2327250	99.3%	2310959	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,037,451	A→G	I219T (ATT→ACT)	recJ ←	ssDNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,037,451	0	A	G	100.0%	131.5 / NA	37	I219T (ATT→ACT)	recJ	ssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/17); total (20/17)

GGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAAT  >  NC_000913/3037322‑3037584

ggACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGGGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGtt                                                                                                                             >  2:1086901/1‑140 (MQ=255)

   cGGCCCTTTCCGGCTCGGATGCGCCTCACCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACg                                                                                                                          <  2:793086/140‑1 (MQ=255)

   cGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACg                                                                                                                          <  2:548094/140‑1 (MQ=255)

        cTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCtcat                                                                                                                     <  1:129469/140‑1 (MQ=255)

                        cGACTCACCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATc                                                                                                     <  2:200265/140‑1 (MQ=255)

                                     gCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTg                                                                                        >  2:38716/1‑140 (MQ=255)

                                         ggTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCa                                                                                    <  1:235317/140‑1 (MQ=255)

                                         ggTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCa                                                                                    >  2:451492/1‑140 (MQ=255)

                                         ggTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCa                                                                                    <  1:451492/140‑1 (MQ=255)

                                          gTCAGAATGCGATTATTGGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAg                                                                                   >  2:538884/1‑140 (MQ=255)

                                          gTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAg                                                                                   >  1:512987/1‑140 (MQ=255)

                                          gTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAg                                                                                   >  1:769920/1‑140 (MQ=255)

                                             aGAATGCGATTATTAGCGCCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAgcgc                                                                                <  2:410778/140‑1 (MQ=255)

                                                aTGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAg                                                                             >  1:814824/1‑140 (MQ=255)

                                                aTGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAg                                                                             >  1:984300/1‑140 (MQ=255)

                                                  gCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCa                                                                           <  2:984300/140‑1 (MQ=255)

                                                    gATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATc                                                                         >  1:993861/1‑140 (MQ=255)

                                                          taGCGCCCCGCGGCCCGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATaa                                                                   <  2:769920/140‑1 (MQ=255)

                                                              gTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACg                                                               <  2:814824/140‑1 (MQ=255)

                                                              gTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACg                                                               >  1:1114089/1‑140 (MQ=255)

                                                               tCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGc                                                              >  2:286439/1‑140 (MQ=255)

                                                                    cGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCacac                                                         >  2:778906/1‑140 (MQ=255)

                                                                           acgTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGcc                                                  <  1:1086901/140‑1 (MQ=255)

                                                                                cGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCa                                             >  2:485624/1‑140 (MQ=255)

                                                                                          cccAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGa                                   <  2:1114089/140‑1 (MQ=255)

                                                                                             aGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACgg                                <  1:538884/140‑1 (MQ=255)

                                                                                                  gACCAGATCCAGCAGTTCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAAt                           >  2:538534/1‑140 (MQ=255)

                                                                                                                 ttCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGt            <  1:38716/140‑1 (MQ=255)

                                                                                                                  tCTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGtt           >  1:33778/1‑140 (MQ=255)

                                                                                                                   cTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCgcgc                <  2:928234/134‑1 (MQ=255)

                                                                                                                   cTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCgcgc                >  1:928234/1‑134 (MQ=255)

                                                                                                                   cTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTa          <  1:538534/140‑1 (MQ=255)

                                                                                                                   cTGCCAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTa          >  2:897372/1‑140 (MQ=255)

                                                                                                                      ccAGGTTAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAggg       >  2:376309/1‑140 (MQ=255)

                                                                                                                           ttAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGt      <  1:334670/136‑1 (MQ=255)

                                                                                                                           ttAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGt      >  2:334670/1‑136 (MQ=255)

                                                                                                                           ttAGGAGTTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAAt  >  2:681453/1‑140 (MQ=255)

GGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATTATTAGCGTCCAGCGGCACGACGTCCGCCACTGTCCCCAGCGCGACCAGATCCAGCAGTTCTGCCAGGTTAGGAATTGCGATGTTACGCTCATCAAACCAGCCCTGATCGCGCAAAAAGGTGCGCAGCGCCAGCATCAGATAAAACGCCACACCCACGCCTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAAT  >  NC_000913/3037322‑3037584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A