Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	150	55.2	2087638	89.3%	1864260	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,002,255	T→C	N637N (AAT→AAC)	xdhA →	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,002,255	0	T	C	100.0%	193.3 / NA	53	N637N (AAT→AAC)	xdhA	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/27); total (26/27)

CTGAAAGCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGAC  >  NC_000913/3002119‑3002389

ctgAAAGCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCa                                                                                                                                     <  2:947127/140‑1 (MQ=255)

ctgAAAGCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCa                                                                                                                                     <  2:258492/140‑1 (MQ=255)

ctgAAAGCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCa                                                                                                                                     <  2:567081/140‑1 (MQ=255)

  gAAAGCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCAct                                                                                                                                   <  1:198184/140‑1 (MQ=255)

      gCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCActgctg                                                                                                                               >  1:804415/1‑140 (MQ=255)

        tCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGc                                                                                                                             >  1:1013551/1‑140 (MQ=255)

        tCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGc                                                                                                                             >  2:769819/1‑140 (MQ=255)

        tCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATAGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGc                                                                                                                             >  1:911156/1‑140 (MQ=255)

          cATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAg                                                                                                                           <  2:657735/140‑1 (MQ=255)

           aTCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGa                                                                                                                          <  1:769819/140‑1 (MQ=255)

                aaCCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTc                                                                                                                     >  2:203067/1‑140 (MQ=255)

                 accaccACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCa                                                                                                                    >  1:707090/1‑140 (MQ=255)

                        cTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTacac                                                                                                             <  2:804415/140‑1 (MQ=255)

                          aaCCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACAcgg                                                                                                           <  1:541128/140‑1 (MQ=255)

                          aaCCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACAcgg                                                                                                           <  1:203067/140‑1 (MQ=255)

                           aCCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACAcggc                                                                                                          <  2:885026/140‑1 (MQ=255)

                               aCCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCggaa                                                                                                      <  1:159090/140‑1 (MQ=255)

                                ccGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGTCAGAAGGTCAGGTACACGGCggaat                                                                                                     >  2:566455/1‑140 (MQ=255)

                                 cGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACAcgg                                                                                                           <  1:49747/133‑1 (MQ=255)

                                 cGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACAcgg                                                                                                           >  2:49747/1‑133 (MQ=255)

                                     gTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCggaatgggaa                                                                                                <  2:663932/140‑1 (MQ=255)

                                       ttGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCggaatgggaatg                                                                                              >  1:470371/1‑140 (MQ=255)

                                              tACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTg                                                                                       >  2:535378/1‑140 (MQ=255)

                                               aCCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTgg                                                                                      >  2:114543/1‑140 (MQ=255)

                                                   ttgttgATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTgg                                                                                  >  2:433666/1‑140 (MQ=255)

                                                     gttgATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGgc                                                                                <  2:1013551/140‑1 (MQ=255)

                                                      ttgATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGgcg                                                                               <  1:433666/140‑1 (MQ=255)

                                                       tgATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGgcgc                                                                              <  1:874190/140‑1 (MQ=255)

                                                       tgATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGgcgc                                                                              <  1:717356/140‑1 (MQ=255)

                                                          tCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTAt                                                                           >  1:661015/1‑140 (MQ=255)

                                                               cGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGaa                                                                      <  2:470371/140‑1 (MQ=255)

                                                                                tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGatcat                                                           <  1:362830/134‑1 (MQ=255)

                                                                                tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGatcat                                                           >  2:362830/1‑134 (MQ=255)

                                                                                tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGc                                                     >  2:170937/1‑140 (MQ=255)

                                                                                tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGc                                                     >  1:598434/1‑140 (MQ=255)

                                                                                tgCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCGGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGc                                                     >  1:903872/1‑140 (MQ=255)

                                                                                        cACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAgcg                                             <  2:704891/140‑1 (MQ=255)

                                                                                          ccATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAgcggc                                           >  1:949964/1‑140 (MQ=255)

                                                                                          ccATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAgcggc                                           >  1:143546/1‑140 (MQ=255)

                                                                                                 ccGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAgcggcg                                          <  1:706856/134‑1 (MQ=255)

                                                                                                 ccGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAgcggcg                                          >  2:706856/1‑134 (MQ=255)

                                                                                                     aTCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTg                                        >  1:645260/1‑132 (MQ=255)

                                                                                                     aTCCTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTg                                        <  2:645260/132‑1 (MQ=255)

                                                                                                       ccTCAACGTTCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAAcc                              >  2:895007/1‑140 (MQ=255)

                                                                                                               tttaTGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCTTTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATctg                      <  2:496231/137‑1 (MQ=255)

                                                                                                               ttCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATctg                      <  2:707090/140‑1 (MQ=255)

                                                                                                               ttCATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATctg                      <  1:170937/140‑1 (MQ=255)

                                                                                                                 cATGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGCCAGGTACACGGCGGAATGGGCATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATctgct                    >  1:845305/1‑140 (MQ=255)

                                                                                                                   tGATTCAGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTgg                  >  2:31446/1‑140 (MQ=255)

                                                                                                                         aGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATctg                      >  2:360805/1‑130 (MQ=255)

                                                                                                                         aGGGCATATTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATctg                      <  1:360805/130‑1 (MQ=255)

                                                                                                                                atTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGcc     >  1:795238/1‑140 (MQ=255)

                                                                                                                                atTCTTAACCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGcc     <  2:143546/140‑1 (MQ=255)

                                                                                                                                   cTTAACCCACTGCGGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGAc  <  2:903872/140‑1 (MQ=255)

CTGAAAGCTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCGGAATGGGAATGGGCATTGGCTGGGCGCTATTTGAAGAGATGATCATCGATGCTAAAAGCGGCGTGGTCCGTAACCCCAATCTGCTGGATTACAAAATGCCGAC  >  NC_000913/3002119‑3002389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A