Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	150	55.2	2087638	89.3%	1864260	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,022,559	T→C	V415A (GTA→GCA)	xdhD →	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,022,559	0	T	C	100.0%	190.1 / NA	53	V415A (GTA→GCA)	xdhD	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/29); total (24/29)

TTGCCGATGCCGATGTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCA  >  NC_000913/3022430‑3022695

ttGCCGATGCCGATGTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCAc                                                                                                                                >  2:379662/1‑140 (MQ=255)

   ccgatgccgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTa                                                                                                                             <  2:462186/140‑1 (MQ=255)

   ccgatgccgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTa                                                                                                                             <  1:379662/140‑1 (MQ=255)

   ccgatgccgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTa                                                                                                                             <  1:270293/140‑1 (MQ=255)

          cgatgTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACgccgcc                                                                                                                      <  2:427640/140‑1 (MQ=255)

                  tCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTcgcg                                                                                                              <  1:330095/140‑1 (MQ=255)

                    aTTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTcgcgcg                                                                                                            <  1:548658/140‑1 (MQ=255)

                         gCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCg                                                                                                       >  2:950496/1‑140 (MQ=255)

                            aaCCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    >  1:242030/1‑140 (MQ=255)

                            aaCCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    >  2:75887/1‑140 (MQ=255)

                            aaCCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    >  2:535085/1‑140 (MQ=255)

                            aaCCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    <  1:532602/140‑1 (MQ=255)

                               cTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCa                                                                                                 <  1:295533/140‑1 (MQ=255)

                                    aCTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGaaa                                                                                            <  2:662549/140‑1 (MQ=255)

                                         aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                                       >  2:963351/1‑140 (MQ=255)

                                         aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                                       <  1:1008949/140‑1 (MQ=255)

                                         aCGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCa                                                                                       >  1:805743/1‑140 (MQ=255)

                                          cGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    <  1:224102/126‑1 (MQ=255)

                                          cGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTgg                                                                                                    >  2:224102/1‑126 (MQ=255)

                                          cGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCAt                                                                                      >  1:1017193/1‑140 (MQ=255)

                                          cGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCAt                                                                                      >  2:451614/1‑140 (MQ=255)

                                                cgcAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGtt                                                                                <  1:497804/140‑1 (MQ=255)

                                                cgcAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGtt                                                                                <  1:535085/140‑1 (MQ=255)

                                                    gcagTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATg                                                                            <  1:937045/140‑1 (MQ=255)

                                                        tGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCAt                                                                        >  1:239204/1‑140 (MQ=255)

                                                            cGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTaaa                                                                    <  1:950496/140‑1 (MQ=255)

                                                             gACTGAACCACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAag                                                                   >  1:545957/1‑140 (MQ=255)

                                                               cTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAagag                                                                 >  1:913568/1‑140 (MQ=255)

                                                                  aaaCACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGa                                                              >  2:481766/1‑140 (MQ=255)

                                                                         tatCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTggcg                                                       >  2:1017579/1‑140 (MQ=255)

                                                                         tatCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTggcg                                                       >  1:279662/1‑140 (MQ=255)

                                                                               cTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGtt                                                 >  2:881878/1‑140 (MQ=255)

                                                                                tttACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGttt                                                >  2:119615/1‑140 (MQ=255)

                                                                                         aTGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCaa                                       >  2:663064/1‑140 (MQ=255)

                                                                                            gACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACa                                    >  1:807391/1‑140 (MQ=255)

                                                                                              cGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAgg                                  <  2:913568/140‑1 (MQ=255)

                                                                                              cGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAgg                                  >  1:841884/1‑140 (MQ=255)

                                                                                                 cGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACa                               <  2:279662/140‑1 (MQ=255)

                                                                                                 cGATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACa                               <  1:75887/140‑1 (MQ=255)

                                                                                                  gATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACAt                              >  2:977338/1‑140 (MQ=255)

                                                                                                  gATCGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACAt                              <  1:481766/140‑1 (MQ=255)

                                                                                                     cGTCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCct                           <  2:203925/140‑1 (MQ=255)

                                                                                                       tCTGGTTATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCctgc                         <  1:977338/140‑1 (MQ=255)

                                                                                                            ttATCCACGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGgaa                    <  2:545957/140‑1 (MQ=255)

                                                                                                                  ccGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAgtg              <  2:805743/139‑1 (MQ=255)

                                                                                                                  aCGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAgtg              <  2:239204/140‑1 (MQ=255)

                                                                                                                  aCGCCTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAgtg              <  2:807391/140‑1 (MQ=255)

                                                                                                                     ccTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgc           >  2:353174/1‑140 (MQ=255)

                                                                                                                     ccTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgc           <  2:1017193/140‑1 (MQ=255)

                                                                                                                      cTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgcg          >  1:6946/1‑140 (MQ=255)

                                                                                                                      cTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgcg          <  1:451614/140‑1 (MQ=255)

                                                                                                                      cTCCACCCAGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTgcg          <  1:119615/140‑1 (MQ=255)

                                                                                                                              aGGCACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCa  <  2:6946/140‑1 (MQ=255)

TTGCCGATGCCGATGTGATCATTGAGCGAACCTATAACTCAACGCAAGCGCAGCAGTGCCCGACTGAAACACATATCTGCTTTACTCGTATGGACGGCGATCGTCTGGTTATCCACGCCTCCACCCAGGTACCATGGCACTTACGCCGCCAGGTCGCGCGCCTCGTGGGCATGAAACAGCATAAAGTTCATGTCATTAAAGAGCGAGTTGGCGGCGGTTTTGGTTCCAAACAGGACATCCTGCTGGAAGAAGTGTGCGCCTGGGCA  >  NC_000913/3022430‑3022695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A