Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	150	55.2	2087638	89.3%	1864260	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,026,663	A→G	T300A (ACC→GCC)	guaD →	guanine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,026,663	0	A	G	100.0%	96.6 / NA	27	T300A (ACC→GCC)	guaD	guanine deaminase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/10); total (17/10)

CATGATGGTTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTA  >  NC_000913/3026528‑3026796

cATGATGGTTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTc                                                                                                                                   <  1:614925/140‑1 (MQ=255)

                     tACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCgg                                                                                                              >  1:154269/1‑140 (MQ=255)

                      aCCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGc                                                                                                             >  1:736909/1‑140 (MQ=255)

                                       aCCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGaaaaa                                                                                            >  1:207144/1‑140 (MQ=255)

                                            tAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCAt                                                                                       >  1:283780/1‑140 (MQ=255)

                                            tAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCAt                                                                                       >  1:651617/1‑140 (MQ=255)

                                            tAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCAt                                                                                       >  2:543267/1‑140 (MQ=255)

                                                   tgtgTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCagaa                                                                                <  2:736909/140‑1 (MQ=255)

                                                            gCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTaa                                                                       >  1:173825/1‑140 (MQ=255)

                                                                 cTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTgg                                                                  >  1:719520/1‑140 (MQ=255)

                                                                   gCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGc                                                                >  1:289638/1‑140 (MQ=255)

                                                                            tcGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACg                                                       >  1:1023118/1‑140 (MQ=255)

                                                                                   aaaaGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCg                                                <  2:30122/140‑1 (MQ=255)

                                                                                      agagTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTg                                             >  2:132808/1‑140 (MQ=255)

                                                                                            ggATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAAGGGATATCGGTGCCGGaa                                       >  2:928601/1‑140 (MQ=255)

                                                                                            ggATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGaa                                       <  2:651617/140‑1 (MQ=255)

                                                                                             gATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAAc                                      >  2:859707/1‑140 (MQ=255)

                                                                                                  tctcAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACtt                                 >  1:275000/1‑140 (MQ=255)

                                                                                                      aGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCaa                             <  1:928601/140‑1 (MQ=255)

                                                                                                      aGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCaa                             <  2:207144/140‑1 (MQ=255)

                                                                                                      aGCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCaa                             >  1:502748/1‑140 (MQ=255)

                                                                                                       gCGAAACCAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAAc                            >  1:800484/1‑140 (MQ=255)

                                                                                                         gAAACTAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACAt                          <  2:195399/140‑1 (MQ=255)

                                                                                                             ccAAATCCAGCATTGCTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACAtgctg                      >  2:843893/1‑140 (MQ=255)

                                                                                                                            cTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGaa       <  2:275000/140‑1 (MQ=255)

                                                                                                                            cTTTCTGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGaa       <  1:843893/140‑1 (MQ=255)

                                                                                                                                 tGTCCGGCCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTa  <  1:859707/140‑1 (MQ=255)

CATGATGGTTATCTGGATGTTTACCATCAGTACGGCCTGACCGGTAAAAACTGTGTCTTTGCTCACTGCGTCCATCTCGAAGAAAAAGAGTGGGATCGTCTCAGCGAAACCAAATCCAGCATTGCTTTCTGTCCGACCTCCAACCTTTACCTCGGCAGCGGCTTATTCAACTTGAAAAAAGCATGGCAGAAGAAAGTTAAAGTGGGCATGGGAACGGATATCGGTGCCGGAACCACTTTCAACATGCTGCAAACGCTGAACGAAGCCTA  >  NC_000913/3026528‑3026796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A