Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	122	83.8	2844814	99.3%	2824900	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,044,704	A→G	D348G (GAC→GGC)	bglA →	6‑phospho‑beta‑glucosidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,044,704	0	A	G	99.0%	349.9 / ‑4.6	96	D348G (GAC→GGC)	bglA	6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (48/47); total (49/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

TGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAA  >  NC_000913/3044567‑3044839

tgtgCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCt                                                                                                                                       <  2:953072/140‑1 (MQ=255)

   gctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTggg                                                                                                                                    >  2:609715/1‑140 (MQ=255)

        gTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTggctggggctgg                                                                                                                               >  2:346895/1‑140 (MQ=255)

        gTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTggctggggctgg                                                                                                                               <  1:950951/140‑1 (MQ=255)

        gTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTggctggggctgg                                                                                                                               <  2:443305/140‑1 (MQ=255)

         tGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGc                                                                                                                              >  1:647842/1‑140 (MQ=255)

          gAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCa                                                                                                                             <  2:606565/140‑1 (MQ=255)

            aGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGa                                                                                                                           <  1:1138044/140‑1 (MQ=255)

             ggCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGAt                                                                                                                          >  1:1289841/1‑140 (MQ=255)

             ggCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGAt                                                                                                                          <  1:775182/140‑1 (MQ=255)

                aCCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGa                                                                                                                       <  2:68798/140‑1 (MQ=255)

                aCCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGa                                                                                                                       <  1:165046/140‑1 (MQ=255)

                 ccTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGAt                                                                                                                      >  2:471058/1‑140 (MQ=255)

                 ccTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGAt                                                                                                                      >  1:1275883/1‑140 (MQ=255)

                     cGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAg                                                                                                                  <  2:724358/140‑1 (MQ=255)

                          aTCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGt                                                                                                             <  2:1360999/140‑1 (MQ=255)

                            cTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCt                                                                                                           <  1:471058/140‑1 (MQ=255)

                               ggTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTgcg                                                                                                        <  2:844829/140‑1 (MQ=255)

                                  ttCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCt                                                                                                           >  1:1048469/1‑134 (MQ=255)

                                  ttCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCt                                                                                                           <  2:1048469/134‑1 (MQ=255)

                                      gCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTAGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCa                                                                                                 >  2:1269167/1‑140 (MQ=255)

                                       cTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCAc                                                                                                >  1:916306/1‑140 (MQ=255)

                                          ttACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACttt                                                                                             >  2:829060/1‑140 (MQ=255)

                                             cATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGACAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCg                                                                                          <  2:1117617/140‑1 (MQ=255)

                                               tGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                        >  1:692028/1‑140 (MQ=255)

                                               tGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                        <  1:150708/140‑1 (MQ=255)

                                                 aCCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACt                                                                                      >  1:547564/1‑140 (MQ=255)

                                                    aaTGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATTTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTa                                                                                   <  2:1289841/140‑1 (MQ=255)

                                                     aTGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTAt                                                                                  >  1:1330289/1‑140 (MQ=255)

                                                     aTGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTAt                                                                                  >  2:1285298/1‑140 (MQ=255)

                                                     aTGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTAt                                                                                  >  1:992578/1‑140 (MQ=255)

                                                     aTGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTAt                                                                                  >  1:129849/1‑140 (MQ=255)

                                                     aTGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTAt                                                                                  >  1:1164671/1‑140 (MQ=255)

                                                     aTGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTAt                                                                                  >  2:953171/1‑140 (MQ=255)

                                                     aTGAAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTAt                                                                                  <  2:96377/140‑1 (MQ=255)

                                                      tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTg                                                                                            >  1:45783/1‑129 (MQ=255)

                                                      tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTg                                                                                            <  2:45783/129‑1 (MQ=255)

                                                      tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                        <  2:859036/133‑1 (MQ=255)

                                                      tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                        >  1:859036/1‑133 (MQ=255)

                                                      tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg                                                                                 >  2:177290/1‑140 (MQ=255)

                                                      tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg                                                                                 >  1:464878/1‑140 (MQ=255)

                                                      tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg                                                                                 >  1:457345/1‑140 (MQ=255)

                                                      tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg                                                                                 <  2:647842/140‑1 (MQ=255)

                                                      tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTCGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg                                                                                 >  1:214830/1‑140 (MQ=255)

                                                       gCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTa                                                                                   >  1:515930/1‑137 (MQ=255)

                                                       gCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTa                                                                                   <  2:515930/137‑1 (MQ=255)

                                                          gTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                        >  2:847663/1‑129 (MQ=255)

                                                          gTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                        <  1:847663/129‑1 (MQ=255)

                                                          gTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCg                                                                             <  2:1013144/140‑1 (MQ=255)

                                                           tGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGt                                                                            <  1:160314/140‑1 (MQ=255)

                                                           tGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGt                                                                            >  2:212211/1‑140 (MQ=255)

                                                           tGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGt                                                                            >  1:374469/1‑140 (MQ=255)

                                                           tGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGt                                                                            >  2:1378776/1‑140 (MQ=255)

                                                           tGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGt                                                                            >  1:1362310/1‑140 (MQ=255)

                                                                gCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                        <  1:629608/123‑1 (MQ=255)

                                                                gCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGaa                                                                                        >  2:629608/1‑123 (MQ=255)

                                                                gCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCa                                                                       >  1:930101/1‑140 (MQ=255)

                                                                gCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCa                                                                       >  1:776751/1‑140 (MQ=255)

                                                                  cGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCaga                                                                     <  1:1285298/140‑1 (MQ=255)

                                                                   gAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCagag                                                                    <  1:1269167/140‑1 (MQ=255)

                                                                    aaGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAgg                                                                   <  2:646781/140‑1 (MQ=255)

                                                                    aaGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAgg                                                                   <  1:786582/140‑1 (MQ=255)

                                                                     aGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGc                                                                  <  2:1069017/140‑1 (MQ=255)

                                                                     aGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGc                                                                  >  2:661050/1‑140 (MQ=255)

                                                                      ggcggcACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGcc                                                                 <  2:916306/140‑1 (MQ=255)

                                                                       gcggcACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCg                                                                <  1:953171/140‑1 (MQ=255)

                                                                       gcggcACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCg                                                                <  1:829060/140‑1 (MQ=255)

                                                                       gcggcACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCg                                                                <  2:568810/140‑1 (MQ=255)

                                                                               ggCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCg                                                                >  2:991465/1‑132 (MQ=255)

                                                                               ggCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCg                                                                <  1:991465/132‑1 (MQ=255)

                                                                                          ctctGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACg                                             <  2:788822/140‑1 (MQ=255)

                                                                                               gTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGtttt                                        >  1:140826/1‑140 (MQ=255)

                                                                                                   tGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGgcg                                    >  2:18412/1‑140 (MQ=255)

                                                                                                       ggCAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTa                                >  2:284235/1‑140 (MQ=255)

                                                                                                         cAGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTTCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACg                              >  2:1120368/1‑140 (MQ=255)

                                                                                                          aGCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGa                             <  2:464878/140‑1 (MQ=255)

                                                                                                           gCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGAc                            <  1:212211/140‑1 (MQ=255)

                                                                                                           gCGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGAc                            <  2:214830/140‑1 (MQ=255)

                                                                                                            cGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACa                           >  1:1322826/1‑140 (MQ=255)

                                                                                                            cGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACa                           <  2:1362310/140‑1 (MQ=255)

                                                                                                            cGTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACa                           >  2:226767/1‑140 (MQ=255)

                                                                                                             gTACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACaa                          <  2:692028/140‑1 (MQ=255)

                                                                                                              tACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACaaa                         <  1:346895/140‑1 (MQ=255)

                                                                                                              tACCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACaaa                         <  2:547564/140‑1 (MQ=255)

                                                                                                               aCCAAACCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAg                        <  2:880451/140‑1 (MQ=255)

                                                                                                                  aaaCCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTgg                     <  1:284235/140‑1 (MQ=255)

                                                                                                                    aCCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGgaa                   <  2:992578/140‑1 (MQ=255)

                                                                                                                    aCCCGTATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGgaa                   <  1:177290/140‑1 (MQ=255)

                                                                                                                         tATGTTAAAGCATTTGGCTGGGGCTGGCAGATTGNTCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAagaaga              >  1:310839/1‑140 (MQ=255)

                                                                                                                         tATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAagaaga              >  2:1302352/1‑140 (MQ=255)

                                                                                                                         tATGTTAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAagaaga              >  1:1127053/1‑140 (MQ=255)

                                                                                                                         tATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAagaaga              >  1:320451/1‑140 (MQ=255)

                                                                                                                             ttAAAGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGgc          <  1:609715/140‑1 (MQ=255)

                                                                                                                               aaaGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGgcag        >  1:837084/1‑140 (MQ=255)

                                                                                                                                aaGCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGATTTGGCGCTTACGACAAAGTGGAAGAAGATGgcagc       >  1:1349184/1‑140 (MQ=255)

                                                                                                                                  gCATCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCAt     <  2:975163/140‑1 (MQ=255)

                                                                                                                                     tCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGTATCaa  >  1:224475/1‑140 (MQ=255)

                                                                                                                                     tCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCaa  >  1:41331/1‑140 (MQ=255)

                                                                                                                                     tCTGGCTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCaa  >  2:985802/1‑140 (MQ=255)

TGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAA  >  NC_000913/3044567‑3044839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A