Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	103	67.9	2307646	99.4%	2293800	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,650	G→A	A434T (GCT→ACT)	xanQ →	xanthine:H(+) symporter XanQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,650	0	G	A	100.0%	137.0 / NA	39	A434T (GCT→ACT)	xanQ	xanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/20); total (19/20)

ACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACA  >  NC_000913/3025519‑3025782

aCGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCggg                                                                                                                               >  1:851692/1‑140 (MQ=255)

 cGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGt                                                                                                                              >  1:324988/1‑140 (MQ=255)

 cGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGt                                                                                                                              >  1:389056/1‑140 (MQ=255)

 cGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTAGTAGTTGAAAACCCTATTTGTACTGGCGGGt                                                                                                                              >  2:705810/1‑140 (MQ=255)

      tAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑t                                                                                                                        >  2:829369/1‑140 (MQ=255)

      tAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑t                                                                                                                        <  2:581794/140‑1 (MQ=255)

          gcgcCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGa                                                                                                                    <  2:324988/140‑1 (MQ=255)

           cgcCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGAt                                                                                                                   <  2:324186/140‑1 (MQ=255)

                          aTTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTAAC‑TTCGCTTTTTTTAAAtattat                                                                                                    >  2:1064432/1‑140 (MQ=255)

                          aTTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGCCCCAC‑CCCGCTTTTTTTTAAtattat                                                                                                    >  2:793366/1‑140 (MQ=255)

                                  cACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCtg                                                                                            <  1:818844/140‑1 (MQ=255)

                                            ggtttaggGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCg                                                                                  <  2:310372/140‑1 (MQ=255)

                                                taggGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTTAT‑TTCGTTTTTTTTTTAAATTTTTCTCCCCGGTGGGTACCGACaa                                                                              >  2:644298/1‑139 (MQ=255)

                                                       tGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTTAA‑TTCGATTTTTTTTAATATTATTCTCCCCCGTGGCTACCCACAGGaaaaaa                                                                       >  2:222224/1‑138 (MQ=255)

                                                         gCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGtt                                                                     <  2:125635/140‑1 (MQ=255)

                                                            tctcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTg                                                                  <  1:974774/140‑1 (MQ=255)

                                                             ctcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGc                                                                 <  1:1064432/140‑1 (MQ=255)

                                                             ctcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGc                                                                 <  1:705810/140‑1 (MQ=255)

                                                              tcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTTACTTGGGATTTT‑TTTAATATTATTCTCCCTCGGGGGTACCCACCGGAAAACAATCTGcc                                                                >  2:176812/1‑140 (MQ=255)

                                                              tcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGcc                                                                <  1:176812/140‑1 (MQ=255)

                                                                        ccGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTTAA‑TTCGGTTTTTTTTAATATTTTTCTCCCCCGTGGGTACCCACAGGAAAAAATTCTGCCTGGGAttttc                                                      >  2:559156/1‑137 (MQ=255)

                                                                         cGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCt                                                     <  1:222224/140‑1 (MQ=255)

                                                                             aTTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGc                                                 <  1:967189/140‑1 (MQ=255)

                                                                                     aaTATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAgaga                                         <  1:1050885/140‑1 (MQ=255)

                                                                                          tGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTAAA‑TTCGATTTTATTTAATATTATTCTCCCCTGTGGCTACCCACAGGAAAAAGTTCTGCCCTGTATTTTCTCAGCGGGAGAGATgggt                                    >  2:854120/1‑138 (MQ=255)

                                                                                           gCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGAtt                                   <  1:990122/140‑1 (MQ=255)

                                                                                           gCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGAtt                                   <  2:851692/140‑1 (MQ=255)

                                                                                                ccTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTTAA‑TGCGAGTTTTTTTAATATTATTCTCCCCTCTGGCTACCGACCAGAAAACGTTTTTCCTGGTATTACCTCCGCGGGAGAGAAGGTTTaaaaa                              >  2:990122/1‑137 (MQ=255)

                                                                                                ccTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTAAA‑TGCGGTTTTTTTTAATATTATTCTCCCCGGTGGGTACCGCCAGGAAAAAGTTCTGCCTGGTATTTCCTCCACGGGAGAGAAGGATTaaaaa                              >  2:1050885/1‑137 (MQ=255)

                                                                                                   ctATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAA‑TGCGGTTTTTTTTAATATTATTCTCCCTCGTGGGTACCCGCCGGAAAAAGGTTTTCCTGGTATTTCCTCAGCGGGAGAGAAGGGTTaaaaaaaa                           >  2:837825/1‑134 (MQ=255)

                                                                                                    tATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTaaa                          <  1:793366/140‑1 (MQ=255)

                                                                                                       ttATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGa                       <  2:389056/140‑1 (MQ=255)

                                                                                                          tGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTAAA‑TGCGATTTTTTTTAATATTATTCTCCCTTGTGGGTACCGACAAGAAAAAGGTCTGCCTGGTATTACCTCCGCGGGAGAGATGGGTTAAAAGTAAAGGAggc                    >  2:978120/1‑138 (MQ=255)

                                                                                                            tATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCaa                  <  1:829369/140‑1 (MQ=255)

                                                                                                             aTTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAat                 <  1:644298/140‑1 (MQ=255)

                                                                                                              ttAGTTGAAAACCCTATTTGTACTGGCGGGGTAAA‑TTCCGTTTTTTTTAATATTATTCTCCCTCGGGGCTACCGCCAGGAAAACGTTCTGCCTGGTATTTCCTCCCCGGAAGAGAGGGGGTAACAGAAAAAGAGGCaaag                >  2:1126395/1‑138 (MQ=255)

                                                                                                                aGTTGAAAACCCTATTTGTACTGGCGGCTTTAA‑TGCGATTTTTTTTAAAATTATCCTCCCCGGTGGGTGCCGACAGGGAAACCTTTCTCCTTGTTTTTCCTCCGCGGAAGAGAGGGATTTAAAATAAAAGAGTCCatgag              >  2:508334/1‑139 (MQ=255)

                                                                                                                 gTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGGTTTTTTTTAATATTATCCTCCCTTGTGGCTACCGACAGGAAAAAAGTTTTCCTGGTATTATCTCAGCGGGAGAGATGGATTAACAGTAAAAGAGGCAataatg             >  2:737085/1‑140 (MQ=255)

                                                                                                                            tATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAaca  <  1:634983/140‑1 (MQ=255)

ACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACA  >  NC_000913/3025519‑3025782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A