Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F48 I1 R1	45	111.1	3780378	99.2%	3750134	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,317,324	A→G	V68A (GTC→GCC)	nusA ←	transcription termination/antitermination protein NusA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,317,324	0	A	G	100.0%	333.8 / NA	86	V68A (GTC→GCC)	nusA	transcription termination/antitermination protein NusA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (47/39); total (47/39)

TAACCTGTTTTGCCGTCTGGGTAGTGATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCG  >  NC_000913/3317187‑3317455

aaaCCTGTTTTGCCGTCTGGGTAGTGATACGGTCAAAGGTGACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCt                                                                                                                                   <  2:764952/139‑1 (MQ=255)

       ttttGCCGTCTGGGTAGTGATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATcaa                                                                                                                            <  1:1095525/140‑1 (MQ=255)

        tttGCCGTCTGGGTAGTGATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATcaac                                                                                                                           >  2:1696107/1‑140 (MQ=255)

         ttGCCGTCTGGGTAGTGATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATcaaca                                                                                                                          >  2:1690208/1‑140 (MQ=255)

          tGCCGTCTGGGTAGTGATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATcaacaa                                                                                                                         <  2:715749/140‑1 (MQ=255)

               tCTGGGTAGTGATACGGTCAAAGGTAACAGGCTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAAc                                                                                                                    <  1:1592571/140‑1 (MQ=255)

                 tGGGTAGTGATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCa                                                                                                                  <  2:1193127/140‑1 (MQ=255)

                         gATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGa                                                                                                          <  1:995128/140‑1 (MQ=255)

                          aTACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGaa                                                                                                         <  2:1492370/140‑1 (MQ=255)

                             cGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAgt                                                                                                      >  2:815319/1‑140 (MQ=255)

                             cGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAgt                                                                                                      >  1:44714/1‑140 (MQ=255)

                               gTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAgtgt                                                                                                    >  2:572399/1‑140 (MQ=255)

                               gTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAgtgt                                                                                                    >  2:826879/1‑140 (MQ=255)

                                 cAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCa                                                                                                  >  2:421971/1‑140 (MQ=255)

                                  aaaGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCaa                                                                                                 >  2:924118/1‑140 (MQ=255)

                                          cAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGGGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGTAAAGTGTCAAAATCAcct                                                                                         >  2:1435689/1‑139 (MQ=255)

                                              cTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCttt                                                                                     <  2:1169417/140‑1 (MQ=255)

                                              cTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCttt                                                                                     >  2:1702009/1‑140 (MQ=255)

                                              cTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCttt                                                                                     >  2:716893/1‑140 (MQ=255)

                                              cTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCttt                                                                                     >  2:1466396/1‑140 (MQ=255)

                                               tCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCtttt                                                                                    >  1:377869/1‑140 (MQ=255)

                                                cAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCttttt                                                                                   >  2:509327/1‑139 (MQ=255)

                                                cAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCttttt                                                                                   >  2:21845/1‑139 (MQ=255)

                                                cAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTg                                                                                   <  1:660137/140‑1 (MQ=255)

                                                 aaTCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGAGTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGc                                                                                  >  2:1681291/1‑140 (MQ=255)

                                                   tCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGcga                                                                                <  1:815319/140‑1 (MQ=255)

                                                    cTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGcgat                                                                               <  1:1652515/140‑1 (MQ=255)

                                                    cTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGcgat                                                                               <  1:826879/140‑1 (MQ=255)

                                                        tCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGcgatcgat                                                                           <  1:1506956/140‑1 (MQ=255)

                                                        tCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGcgatcgat                                                                           <  1:421971/140‑1 (MQ=255)

                                                        tCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGcgatcgat                                                                           <  2:204643/140‑1 (MQ=255)

                                                        tCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGcgatcgat                                                                           <  1:572399/140‑1 (MQ=255)

                                                        tCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGcgatcgat                                                                           >  1:816328/1‑140 (MQ=255)

                                                           tCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTg                                                                        >  2:984370/1‑140 (MQ=255)

                                                             aaCGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTa                                                                      >  1:64132/1‑140 (MQ=255)

                                                             aaCGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTa                                                                      >  1:1701015/1‑140 (MQ=255)

                                                               agTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCATTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTAcg                                                                    <  2:1424151/139‑1 (MQ=255)

                                                                 tAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTAcgcg                                                                  >  1:1793835/1‑140 (MQ=255)

                                                                  aaTCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCgg                                                                 <  2:334967/140‑1 (MQ=255)

                                                                  aaTCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCgg                                                                 <  2:461156/140‑1 (MQ=255)

                                                                  aaTCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCgg                                                                 <  1:984370/140‑1 (MQ=255)

                                                                  aaTCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCgg                                                                 <  2:473888/140‑1 (MQ=255)

                                                                   aTCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGa                                                                <  1:810500/140‑1 (MQ=255)

                                                                   aTCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGa                                                                >  1:664326/1‑140 (MQ=255)

                                                                   aTCGCCCAGGTTCAGGCTTTCATCTTCATAACATGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGa                                                                <  1:1455055/140‑1 (MQ=255)

                                                                    tCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGAc                                                               >  1:1751171/1‑140 (MQ=255)

                                                                        ccAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCg                                                           <  1:1702009/140‑1 (MQ=255)

                                                                          aGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGAt                                                         <  2:127526/140‑1 (MQ=255)

                                                                           ggTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGAtc                                                        >  1:1221712/1‑140 (MQ=255)

                                                                           ggTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGAtc                                                        >  1:942676/1‑140 (MQ=255)

                                                                           ggTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGAtc                                                        >  1:273310/1‑140 (MQ=255)

                                                                                aGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTg                                                   >  1:1880446/1‑140 (MQ=255)

                                                                                  gCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGtt                                                 <  2:779456/140‑1 (MQ=255)

                                                                                    tttCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCa                                               <  1:1696107/140‑1 (MQ=255)

                                                                                      tCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCata                                             >  1:1682193/1‑140 (MQ=255)

                                                                                      tCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCata                                             >  1:402288/1‑140 (MQ=255)

                                                                                       cATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCatat                                            >  2:1704298/1‑140 (MQ=255)

                                                                                       cATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCatat                                            <  2:273310/140‑1 (MQ=255)

                                                                                          cTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATAtttt                                         <  1:28070/140‑1 (MQ=255)

                                                                                          cTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATAtttt                                         <  2:1793835/140‑1 (MQ=255)

                                                                                            tCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATAtttttt                                       >  2:1860408/1‑140 (MQ=255)

                                                                                                 aCGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTttg                                  <  2:1068015/140‑1 (MQ=255)

                                                                                                    tGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTttgttt                               >  2:53222/1‑139 (MQ=255)

                                                                                                    tGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTttgttg                               >  1:772420/1‑140 (MQ=255)

                                                                                                    tGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTttgttg                               >  2:250955/1‑140 (MQ=255)

                                                                                                      cGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCt                             >  2:211874/1‑140 (MQ=255)

                                                                                                      cGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCt                             >  2:1888263/1‑140 (MQ=255)

                                                                                                      cGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCt                             >  2:1035433/1‑140 (MQ=255)

                                                                                                       ggCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTg                            >  1:1863702/1‑140 (MQ=255)

                                                                                                       ggCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTg                            >  2:838858/1‑140 (MQ=255)

                                                                                                       ggCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTg                            >  1:472105/1‑140 (MQ=255)

                                                                                                        gCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGt                           <  1:250955/140‑1 (MQ=255)

                                                                                                              aGGGTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAg                     >  1:1657333/1‑140 (MQ=255)

                                                                                                               gggTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAgc                    >  2:1073321/1‑140 (MQ=255)

                                                                                                                ggTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAgcg                   <  2:942676/140‑1 (MQ=255)

                                                                                                                ggTGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCATTGTTGCTGTCGCCAgcg                   <  1:1704298/140‑1 (MQ=255)

                                                                                                                  tGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAgcgcg                 <  2:1701015/140‑1 (MQ=255)

                                                                                                                  tGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAgcgcg                 <  1:1466396/140‑1 (MQ=255)

                                                                                                                  tGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAgcgcg                 <  1:716893/140‑1 (MQ=255)

                                                                                                                  tGATTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGATTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAgcgcg                 <  1:1435689/140‑1 (MQ=255)

                                                                                                                    aTTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCt               >  1:1022778/1‑140 (MQ=255)

                                                                                                                    aTTTCCTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCt               >  1:629952/1‑140 (MQ=255)

                                                                                                                         cTTGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCa          >  1:247285/1‑140 (MQ=255)

                                                                                                                           tGGTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAAt        >  2:949175/1‑140 (MQ=255)

                                                                                                                            ggTCGGCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATg       >  1:809274/1‑140 (MQ=255)

                                                                                                                                 gCTGGGTGGCTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGACGCCAGCGCGCTTTCCAATGCTTCg  <  2:472105/140‑1 (MQ=255)

TAACCTGTTTTGCCGTCTGGGTAGTGATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCG  >  NC_000913/3317187‑3317455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A