Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F48 I1 R1	45	111.1	3780378	99.2%	3750134	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,224,158	G→T	A111S (GCG→TCG)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,224,158	0	G	T	98.6%	247.0 / ‑3.6	73	A111S (GCG→TCG)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (30/42); total (31/42)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

CCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGGCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGA  >  NC_000913/4224021‑4224297

ccgcTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGtcg                                                                                                                                           <  1:785202/140‑1 (MQ=255)

 cgcTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGtcgc                                                                                                                                          >  1:412311/1‑140 (MQ=255)

   cTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGTCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGa                                                                                                                                        >  1:1287078/1‑140 (MQ=255)

     aTCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGc                                                                                                                                      <  1:1031080/140‑1 (MQ=255)

          caACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTtgtg                                                                                                                                 <  1:72423/140‑1 (MQ=255)

            aCGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCt                                                                                                                               <  2:1725644/140‑1 (MQ=255)

            aCGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCt                                                                                                                               <  2:1611913/140‑1 (MQ=255)

            aCGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCt                                                                                                                               <  2:1269681/140‑1 (MQ=255)

            aCGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCt                                                                                                                               <  1:502164/140‑1 (MQ=255)

             cGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTg                                                                                                                              >  2:633579/1‑140 (MQ=255)

             cGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTg                                                                                                                              >  1:838345/1‑140 (MQ=255)

                cTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCt                                                                                                                               <  2:317269/136‑1 (MQ=255)

                cTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCt                                                                                                                               >  1:317269/1‑136 (MQ=255)

                cTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGAct                                                                                                                           >  2:1158545/1‑139 (MQ=255)

                cTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACg                                                                                                                           >  1:81417/1‑140 (MQ=255)

                cTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACg                                                                                                                           >  2:731391/1‑140 (MQ=255)

                cTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACg                                                                                                                           >  2:1013163/1‑140 (MQ=255)

                cTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACg                                                                                                                           >  1:1139241/1‑140 (MQ=255)

                  aCTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAg                                                                                                                         >  2:812078/1‑140 (MQ=255)

                        aaGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAGCTGTCGCGAGCTTGTGCTGACGAGTGGAcc                                                                                                                   <  1:1050493/140‑1 (MQ=255)

                             ggCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACcgcgcg                                                                                                              >  2:804779/1‑140 (MQ=255)

                             ggCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACcgcgcg                                                                                                              <  2:223661/140‑1 (MQ=255)

                             ggCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACcgcgcg                                                                                                              <  1:1152381/140‑1 (MQ=255)

                             ggCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACcgcgcg                                                                                                              >  2:1334868/1‑140 (MQ=255)

                             ggCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACcgcgcg                                                                                                              <  2:1422772/140‑1 (MQ=255)

                                        catcGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAgaga                                                                                                   <  1:1318112/140‑1 (MQ=255)

                                        aatcGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAgaga                                                                                                   <  2:1462600/139‑1 (MQ=255)

                                              aaCCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACcgc                                                                                             >  1:62598/1‑140 (MQ=255)

                                                ccAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCgcgc                                                                                           <  1:883112/140‑1 (MQ=255)

                                                     acCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGt                                                                                      <  1:73396/140‑1 (MQ=255)

                                                      ccTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGtt                                                                                     <  2:1661262/140‑1 (MQ=255)

                                                         tCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGcc                                                                                  >  2:1073706/1‑140 (MQ=255)

                                                          cAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCg                                                                                 >  2:211453/1‑140 (MQ=255)

                                                          cAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCg                                                                                 >  2:980860/1‑140 (MQ=255)

                                                           aaCTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCgg                                                                                <  1:812078/140‑1 (MQ=255)

                                                           aaCTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCgg                                                                                >  2:1305387/1‑140 (MQ=255)

                                                           aaCTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCgg                                                                                <  2:412311/140‑1 (MQ=255)

                                                               ccACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGtt                                                                            <  1:804779/140‑1 (MQ=255)

                                                               ccACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGtt                                                                            >  1:1153733/1‑140 (MQ=255)

                                                                cACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGGTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGttt                                                                           >  1:392019/1‑139 (MQ=255)

                                                                cACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTtc                                                                           <  2:81417/140‑1 (MQ=255)

                                                                 aCGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGCGCTTGTGCTGACGAGTGGTCCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGGGtttt                                                                          >  2:95349/1‑138 (MQ=255)

                                                                 aCGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTtct                                                                          >  1:1870154/1‑140 (MQ=255)

                                                                 aCGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTtct                                                                          >  2:210214/1‑140 (MQ=255)

                                                                 aCGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTtct                                                                          >  2:1824495/1‑140 (MQ=255)

                                                                   gACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCg                                                                        >  2:1345460/1‑140 (MQ=255)

                                                                    aCCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCgg                                                                       <  1:1824495/140‑1 (MQ=255)

                                                                        ttGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCg                                                                   >  2:37057/1‑140 (MQ=255)

                                                                                gCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCg                                                           <  1:1748281/140‑1 (MQ=255)

                                                                                 cGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGc                                                          >  1:165480/1‑140 (MQ=255)

                                                                                 cGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGc                                                          <  2:1395507/140‑1 (MQ=255)

                                                                                  ggATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCa                                                         <  2:22681/140‑1 (MQ=255)

                                                                                  ggATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCa                                                         <  1:37057/140‑1 (MQ=255)

                                                                                  ggATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCa                                                         <  1:206120/140‑1 (MQ=255)

                                                                                  ggATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCa                                                         <  2:838345/140‑1 (MQ=255)

                                                                                  ggATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCa                                                         <  1:892462/140‑1 (MQ=255)

                                                                                    aTTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACg                                                       <  2:1595867/140‑1 (MQ=255)

                                                                                    aTTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACg                                                       <  1:1013163/140‑1 (MQ=255)

                                                                                    aTTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACg                                                       <  1:2650/140‑1 (MQ=255)

                                                                                       aCCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCg                                                    <  2:1153733/140‑1 (MQ=255)

                                                                                         cAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTc                                                  <  2:1448552/140‑1 (MQ=255)

                                                                                          aGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCt                                                 >  1:328753/1‑140 (MQ=255)

                                                                                          aGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGGCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCt                                                 >  2:875206/1‑140 (MQ=255)

                                                                                            aTGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTAt                                               <  1:1334868/140‑1 (MQ=255)

                                                                                                aaTCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTtct                                           >  1:628429/1‑140 (MQ=255)

                                                                                                 aTCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTtctc                                          <  2:1287078/140‑1 (MQ=255)

                                                                                                   cccTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCg                                        >  1:405805/1‑140 (MQ=255)

                                                                                                   cccTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCg                                        <  1:1073706/140‑1 (MQ=255)

                                                                                                      tGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTTGCGGGCTTTTGCTGATGAGTGGACCGCGTGCACTCCAGAGAAACCGTGTTTTGTTGCCTGTGTTTTCTTTCCGACCAACCGCACTGGGTCTATTTCTCCGGat                                     >  2:1111025/1‑139 (MQ=255)

                                                                                                      tGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGAc                                     <  1:1158545/140‑1 (MQ=255)

                                                                                                       gTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACg                                    >  1:668682/1‑140 (MQ=255)

                                                                                                           gcggAAATCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCaa                                <  1:474171/140‑1 (MQ=255)

                                                                                                                  tCAACTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCg                         <  2:1148449/140‑1 (MQ=255)

                                                                                                                     aCTTTGCGGCGGCGAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCa                      <  2:611965/140‑1 (MQ=255)

                                                                                                                              cggcgAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAat             >  2:773217/1‑140 (MQ=255)

                                                                                                                              cggcgAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAat             <  1:1345460/140‑1 (MQ=255)

                                                                                                                                gcgAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAatat           >  2:262284/1‑140 (MQ=255)

                                                                                                                                gcgAAACTGTCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGACATTTCGTAatat           <  2:165480/140‑1 (MQ=255)

                                                                                                                                         gcgcGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGa  <  1:875206/140‑1 (MQ=255)

CCGCTATCCACAACGCCTACTTTGAAGCGGGCGCGGATATCATCGAAACCAACACCTTCAACTCCACGACCATTGCGATGGCGGATTACCAGATGGAATCCCTGTCGGCGGAAATCAACTTTGCGGCGGCGAAACTGGCGCGAGCTTGTGCTGACGAGTGGACCGCGCGCACGCCAGAGAAACCGCGCTACGTTGCCGGTGTTCTCGGCCCGACCAACCGCACGGCGTCTATTTCTCCGGACGTCAACGATCCGGCATTTCGTAATATCACTTTTGA  >  NC_000913/4224021‑4224297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A