Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,224,754	C→A	G309G (GGC→GGA)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,224,754	0	C	A	100.0%	106.0 / NA	32	G309G (GGC→GGA)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/15); total (17/15)

CGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGC  >  NC_000913/4224621‑4224880

cGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCtgt                                                                                                                          >  1:466916/1‑140 (MQ=255)

            tCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCca                                                                                                              <  2:72360/140‑1 (MQ=255)

             cACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCcac                                                                                                             >  1:561729/1‑140 (MQ=255)

                  cgcACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACat                                                                                                        >  1:381503/1‑140 (MQ=255)

                                 ggCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGt                                                                                         >  1:657793/1‑140 (MQ=255)

                                 ggCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGt                                                                                         >  2:109538/1‑140 (MQ=255)

                                    tACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGt                                                                                      <  2:381503/140‑1 (MQ=255)

                                     aCCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGTACCACGCCACAACATATTGCAGCGATGAGTCGTg                                                                                     <  2:277867/140‑1 (MQ=255)

                                     aCCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTg                                                                                     <  2:561729/140‑1 (MQ=255)

                                     aCCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTg                                                                                     <  2:466916/140‑1 (MQ=255)

                                            gCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGa                                                                              <  2:819747/140‑1 (MQ=255)

                                              cTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAg                                                                            >  1:142960/1‑140 (MQ=255)

                                                        tACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGcgcc                                                                  >  2:670652/1‑140 (MQ=255)

                                                           gATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCcgcg                                                               >  2:488199/1‑140 (MQ=255)

                                                                  aCGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTg                                                        >  2:344306/1‑140 (MQ=255)

                                                                    gCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGcc                                                      <  2:142960/140‑1 (MQ=255)

                                                                         cacGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGaaa                                                 <  1:488199/140‑1 (MQ=255)

                                                                                     aCAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCt                                     <  1:109538/140‑1 (MQ=255)

                                                                                        gATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGcc                                  >  1:731590/1‑140 (MQ=255)

                                                                                             gTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTg                             >  1:579438/1‑140 (MQ=255)

                                                                                               aaaTGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTc                           <  2:142123/139‑1 (MQ=255)

                                                                                                aaTGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTcc                          >  1:517687/1‑140 (MQ=255)

                                                                                                    ggCGCAAGCGGGTTTTCTCAATATCGTCAGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGcc                      <  2:802876/140‑1 (MQ=255)

                                                                                                       gcAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTgg                   <  2:400472/140‑1 (MQ=255)

                                                                                                        cAAGCGGGTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGa                  >  1:365439/1‑140 (MQ=255)

                                                                                                               gTTTTCTCAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTg           >  2:199163/1‑140 (MQ=255)

                                                                                                                   tctcAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACa       >  2:615229/1‑140 (MQ=255)

                                                                                                                   tctcAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACa       <  2:731590/140‑1 (MQ=255)

                                                                                                                   tctcAATATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACa       <  1:748446/140‑1 (MQ=255)

                                                                                                                       aaTATCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTgg   <  2:365439/140‑1 (MQ=255)

                                                                                                                        atatCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGc  >  2:283137/1‑140 (MQ=255)

                                                                                                                         tatCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACa       >  1:673754/1‑134 (MQ=255)

                                                                                                                         tatCGTCGGCGGATGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACa       <  2:673754/134‑1 (MQ=255)

CGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGC  >  NC_000913/4224621‑4224880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A