Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F48 I1 R1	45	111.1	3780378	99.2%	3750134	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,357,514	C→A	L368L (CTG→CTT)	cadA ←	lysine decarboxylase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,357,514	0	C	A	98.5%	480.8 / ‑1.0	135	L368L (CTG→CTT)	cadA	lysine decarboxylase 1
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (83/50); total (84/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 136 total aligned reads displayed.

CCGCAGCGGTTTCAGTGGACGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCAC  >  NC_000913/4357378‑4357648

      cGGTTTCAGTGGACGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGa                                                                                                                               <  1:1804680/140‑1 (MQ=255)

      cGGTTTCAGTGGACGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGa                                                                                                                               <  1:61701/140‑1 (MQ=255)

      cGGTTTCAGTGGACGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGa                                                                                                                               <  2:830455/140‑1 (MQ=255)

        gTTTCAGTGGACGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGt                                                                                                                             <  1:470939/140‑1 (MQ=255)

           tCAGTGGACGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGa                                                                                                                          <  2:676759/140‑1 (MQ=255)

           tCAGTGGACGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGa                                                                                                                          <  1:83941/140‑1 (MQ=255)

                  aCGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGa                                                                                                                               >  2:326799/1‑128 (MQ=255)

                   cGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTAAGTGGACTGGGttt                                                                                                                  >  2:1622894/1‑140 (MQ=255)

                       aCGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTa                                                                                                              >  2:1591883/1‑140 (MQ=255)

                              cGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACt                                                                                                       <  1:1591883/140‑1 (MQ=255)

                                tAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACttt                                                                                                     >  2:1463770/1‑140 (MQ=255)

                                   tGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTccc                                                                                                  >  2:886562/1‑140 (MQ=255)

                                   tGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTccc                                                                                                  >  2:1416654/1‑140 (MQ=255)

                                    gCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCt                                                                                                 <  1:1790083/140‑1 (MQ=255)

                                     cGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCtt                                                                                                >  2:1307209/1‑140 (MQ=255)

                                     cGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCtt                                                                                                >  2:730678/1‑140 (MQ=255)

                                     cGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCtt                                                                                                >  2:1097738/1‑140 (MQ=255)

                                     cGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCtt                                                                                                >  1:1072662/1‑140 (MQ=255)

                                     cGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCtt                                                                                                >  2:1256054/1‑140 (MQ=255)

                                       gagaAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACtt                                                                                                      <  2:901706/132‑1 (MQ=255)

                                       gagaAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACtt                                                                                                      >  1:901706/1‑132 (MQ=255)

                                       gagaAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCt                                                                                              >  2:1814810/1‑140 (MQ=255)

                                                  gtgtGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCAcc                                                                                   >  2:66676/1‑140 (MQ=255)

                                                  gtgtGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCAcc                                                                                   >  2:1335076/1‑140 (MQ=255)

                                                    gtgCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGc                                                                                 >  2:453562/1‑140 (MQ=255)

                                                     tgCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCt                                                                                >  2:324955/1‑140 (MQ=255)

                                                     tgCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACCTTCCCTTCTACACGGCCACCGCt                                                                                >  1:841571/1‑140 (MQ=255)

                                                      gCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTc                                                                               >  1:864829/1‑140 (MQ=255)

                                                         tcatGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATa                                                                            <  1:886562/140‑1 (MQ=255)

                                                         tcatGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATa                                                                            >  1:1030251/1‑140 (MQ=255)

                                                          catGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATAc                                                                           >  2:1880952/1‑140 (MQ=255)

                                                            tGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCg                                                                         >  1:384843/1‑140 (MQ=255)

                                                            tGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCg                                                                         >  2:289039/1‑140 (MQ=255)

                                                               aGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATa                                                                            >  2:154608/1‑134 (MQ=255)

                                                               aGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATa                                                                            <  1:154608/134‑1 (MQ=255)

                                                                 gCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCAttt                                                                    >  1:1473889/1‑140 (MQ=255)

                                                                   ttCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCCTACCGCATTTAc                                                                  <  2:1259843/140‑1 (MQ=255)

                                                                   ttCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTAc                                                                  <  2:1275981/140‑1 (MQ=255)

                                                                    tCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTAcc                                                                 >  2:1639931/1‑140 (MQ=255)

                                                                    tCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTAcc                                                                 >  1:744906/1‑140 (MQ=255)

                                                                    tCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTAcc                                                                 >  2:531867/1‑140 (MQ=255)

                                                                    tCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTAcc                                                                 >  1:1525807/1‑140 (MQ=255)

                                                                     cGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCt                                                                >  1:11840/1‑140 (MQ=255)

                                                                      gTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCAtt                                                                     >  2:1455529/1‑134 (MQ=255)

                                                                      gTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCtt                                                               >  2:615742/1‑140 (MQ=255)

                                                                      gTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCtt                                                               >  1:143477/1‑140 (MQ=255)

                                                                       ttAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTc                                                              >  1:162940/1‑140 (MQ=255)

                                                                           aGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTaa                                                          >  1:900326/1‑140 (MQ=255)

                                                                           aGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTaa                                                          >  2:1661341/1‑140 (MQ=255)

                                                                                tcttcGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCgt                                                     >  2:1577678/1‑139 (MQ=255)

                                                                                tcttcGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCgg                                                     >  1:461449/1‑140 (MQ=255)

                                                                                 cttcGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGt                                                    >  1:1392380/1‑140 (MQ=255)

                                                                                 cttcGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGt                                                    <  2:1320262/140‑1 (MQ=255)

                                                                                        tACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGt                                             <  1:105162/140‑1 (MQ=255)

                                                                                          cGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTg                                           >  1:730510/1‑140 (MQ=255)

                                                                                              aCCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGgtgt                                       >  2:1367183/1‑140 (MQ=255)

                                                                                                cTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTaa                                     >  2:333068/1‑140 (MQ=255)

                                                                                                   tAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAATTTGGTGTAAgtt                                  >  2:213398/1‑138 (MQ=255)

                                                                                                   tAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGc                                  >  2:102223/1‑140 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           <  2:1129804/140‑1 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           <  2:215401/140‑1 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           >  1:413006/1‑140 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           >  1:22692/1‑140 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           <  2:49663/140‑1 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           >  1:1641086/1‑140 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           >  1:705522/1‑140 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           >  1:803319/1‑140 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           <  1:1463770/140‑1 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           <  1:1307209/140‑1 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           >  1:1180745/1‑140 (MQ=255)

                                                                                                          gATCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGGTTGGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAAGCGGTGAGAAGTTGGTGTAAGGCACCCAcg                           >  1:1776159/1‑140 (MQ=255)

                                                                                                           aTCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcgc                          <  1:999567/140‑1 (MQ=255)

                                                                                                            tCATGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCAcgcg                         <  2:864829/140‑1 (MQ=255)

                                                                                                               tGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAg                      >  1:857710/1‑140 (MQ=255)

                                                                                                               tGGAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCATCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAg                      >  1:56723/1‑140 (MQ=255)

                                                                                                                 gAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTc                    <  1:1829605/140‑1 (MQ=255)

                                                                                                                 gAAGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTc                    <  2:1805449/140‑1 (MQ=255)

                                                                                                                  aaGCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCa                   <  1:289039/140‑1 (MQ=255)

                                                                                                                    gCCTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCaaa                 >  2:1879671/1‑140 (MQ=255)

                                                                                                                     ccTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAg                >  1:1576138/1‑140 (MQ=255)

                                                                                                                     ccTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAg                >  1:401233/1‑140 (MQ=255)

                                                                                                                     ccTGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAg                >  2:1400363/1‑140 (MQ=255)

                                                                                                                       tGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtt              >  2:21183/1‑139 (MQ=255)

                                                                                                                       tGAGAGAACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtg              >  2:1661693/1‑140 (MQ=255)

                                                                                                                        gagagaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtgg             <  1:1097738/140‑1 (MQ=255)

                                                                                                                         agagaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtgga            >  2:1758624/1‑140 (MQ=255)

                                                                                                                         agagaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtgga            <  2:1046239/140‑1 (MQ=255)

                                                                                                                         agagaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtgga            >  1:593652/1‑140 (MQ=255)

                                                                                                                         agagaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtgga            <  1:997365/140‑1 (MQ=255)

                                                                                                                          gagaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtggat           <  1:1817144/140‑1 (MQ=255)

                                                                                                                          aagaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtggat           <  2:162940/139‑1 (MQ=255)

                                                                                                                          aagaACGCCCCCAGAAATTTGTGAGTGGAATTGGTTTCGTAAATCACTTTCCCTTCTACGCGGCCACCGCTCATACCCCATTTACCTTCGTAAAGCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtggat           <  1:213398/139‑1 (MQ=255)

                                                                                                                           agaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtggatg          <  1:1400363/140‑1 (MQ=255)

                                                                                                                            gaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtggatgg         <  2:1180745/140‑1 (MQ=255)

                                                                                                                            gaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtggatgg         <  2:669102/140‑1 (MQ=255)

                                                                                                                            gaACGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtggatgg         <  1:1001477/140‑1 (MQ=255)

                                                                                                                            aaaCGCCGCCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtggatgg         <  2:1030251/139‑1 (MQ=255)

                                                                                                                               cgccgcCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGAtt      >  1:442035/1‑140 (MQ=255)

                                                                                                                               cgccgcCAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGAtt      >  1:1014784/1‑140 (MQ=255)

                                                                                                                                   gccAGAAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTcac  >  2:393238/1‑140 (MQ=255)

CCGCAGCGGTTTCAGTGGACGCCACGATACCGTAGTGCGGAGAAGTGGTGGTGTGCATCATGTAGGCTTCGTTAAAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCAC  >  NC_000913/4357378‑4357648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A