Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F48 I1 R1 90 35.3 1199032 98.5% 1181046 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,952,872 G→C D1044E (GAC→GAG)  recB ← exodeoxyribonuclease V subunit RecB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,952,8720GC100.0% 36.8 / NA 11D1044E (GAC→GAG) recBexodeoxyribonuclease V subunit RecB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (1/10);  total (1/10)

ACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAGGCTTACGCCGGTTTCATTGAGAGGTGCC  >  NC_000913/2952748‑2953007
                                                                                                                            |                                                                                                                                       
aCCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATTGCGGCTCAAACTGGCGGAtt                                                                                                                          >  1:152286/1‑140 (MQ=255)
aCCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGAtt                                                                                                                          >  2:507575/1‑140 (MQ=255)
      tGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGATTAACGTa                                                                                                                    <  2:157781/140‑1 (MQ=255)
            tataGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGGTTAACGTATCaaac                                                                                                              >  2:28596/1‑138 (MQ=255)
                   gAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCCACCTGCCGATAGCGGCTCAAACTTGCCGGTTTACGTACCAAGCTGACtcg                                                                                                       >  2:393299/1‑138 (MQ=255)
                        ggTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATa                                                                                                  >  1:285577/1‑140 (MQ=255)
                          taataaCGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCGTGCGGATAGCGGCTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAg                                                                                                <  1:57352/140‑1 (MQ=255)
                          taataaCGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAg                                                                                                <  1:347934/140‑1 (MQ=255)
                                   ccTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGGTTAACGTATCAAACTGACTGGCGATAAGCGGGTCACt                                                                                       >  2:308046/1‑140 (MQ=255)
                                    cTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGGTTAACGTATCAAGCTGACTGGGGGTAAACGGTTTACtt                                                                                      >  2:216756/1‑139 (MQ=255)
                                      tCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAAt                                                                                    <  1:311922/140‑1 (MQ=255)
                                          ggCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCTGCTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGc                                                                                <  2:285577/140‑1 (MQ=255)
                                                           aTAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCAtctc                                                               <  1:550301/140‑1 (MQ=255)
                                                                                        cATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGCTCAAACTGGCGGATTAACGTATCAAGCTGGCTGGCGATAAGCGGTTCACTAATCGGGAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGAc                                  <  1:387999/140‑1 (MQ=255)
                                                                                                      gcggGCAGCCTGCGGATAGCGGCTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAGGCTTACGCCg                    <  1:507575/140‑1 (MQ=255)
                                                                                                       cggGCAGCCTTCGGATAGCGGCTCAAACTGGCGGATTAAAGTATCAAGCTGACTGGCGATTAGCGGTTCACCAATCGGCAGATAAAAATCCATCTTCACCTGTTTATTTCGGGCGGGAAGTTGAATCAGGCTTACGCCgg                   >  2:313801/1‑140 (MQ=255)
                                                                                                                   ggATAGCGGCTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAGGCTTACGCCGGTTTCATTGagag       <  2:84044/140‑1 (MQ=255)
                                                                                                                        gCGGCTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAGGCTTACGCCGGTTTCATTGAGAGGTGcc  <  1:313801/140‑1 (MQ=255)
                                                                                                                            |                                                                                                                                       
ACCAGTTGGATTTATAGTCGAGCAGGTAATAACGCCCTTCGTGGCGGAACACCAGGTCGATAAAGCCTTTTAACATGCCACGTACCTGCATGAACTCCAGCGGCGGGCAGCCTGCGGATAGCGGGTCAAACTGGCGGATTAACGTATCAAGCTGACTGGCGATAAGCGGTTCACTAATCGGCAGATAAAACTCCATCTCCACCTGTTTATTGCGGGCGGAAAGTTGACTCAGGCTTACGCCGGTTTCATTGAGAGGTGCC  >  NC_000913/2952748‑2953007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A