Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,954,431	G→T	Q525K (CAA→AAA)	recB ←	exodeoxyribonuclease V subunit RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,954,431	0	G	T	100.0%	106.3 / NA	31	Q525K (CAA→AAA)	recB	exodeoxyribonuclease V subunit RecB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/14); total (17/14)

GGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTGCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGC  >  NC_000913/2954294‑2954563

ggCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTtcg                                                                                                                                    <  1:801119/140‑1 (MQ=255)

     cgcgCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCgcagca                                                                                                                               >  1:268666/1‑140 (MQ=255)

     cgcgCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCgcagca                                                                                                                               >  1:689741/1‑140 (MQ=255)

      gcgcACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGcac                                                                                                                              >  2:516066/1‑140 (MQ=255)

               ggcggcCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTgcg                                                                                                                     <  2:482260/140‑1 (MQ=255)

               ggcggcCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTgcg                                                                                                                     <  2:125655/140‑1 (MQ=255)

                  ggcCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCa                                                                                                                  <  2:48608/140‑1 (MQ=255)

                  ggcCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCa                                                                                                                  <  1:517874/140‑1 (MQ=255)

                  ggcCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCa                                                                                                                  <  1:67966/140‑1 (MQ=255)

                    cctcctGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATg                                                                                                                <  1:365942/140‑1 (MQ=255)

                      tcctGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGt                                                                                                              >  1:296655/1‑140 (MQ=255)

                                  cgcACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATc                                                                                                  >  2:189037/1‑140 (MQ=255)

                                          cacaCTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAAcgc                                                                                          >  1:430609/1‑140 (MQ=255)

                                                 aTGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCt                                                                                   <  1:31291/140‑1 (MQ=255)

                                                   gTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCttt                                                                                 <  2:485063/140‑1 (MQ=255)

                                                    tCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTc                                                                                >  2:699558/1‑140 (MQ=255)

                                                        aaGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCt                                                                            >  1:342948/1‑140 (MQ=255)

                                                           cacgcacCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTcc                                                                         >  1:728638/1‑140 (MQ=255)

                                                              gcacCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATc                                                                      >  2:457935/1‑140 (MQ=255)

                                                               cacCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCa                                                                     >  1:443217/1‑140 (MQ=255)

                                                                            tcgtcgCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCAt                                                        >  2:55050/1‑140 (MQ=255)

                                                                              gtcgCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCg                                                      <  1:578819/140‑1 (MQ=255)

                                                                                   cgagcatcaGCATCGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTTCGCCATGATCCTTTGATAATCTCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCCGGc                                                 <  2:397019/136‑1 (MQ=255)

                                                                                   cGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACACCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGc                                                 <  1:806731/140‑1 (MQ=255)

                                                                                       catcaGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCtgtt                                             >  2:410325/1‑139 (MQ=255)

                                                                                            gCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCa                                        <  2:342948/140‑1 (MQ=255)

                                                                                                  cTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTa                                  >  2:313407/1‑140 (MQ=255)

                                                                                                        cccGCTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTAAATACa                            <  2:73926/140‑1 (MQ=255)

                                                                                                            cTGTCCGGCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGAACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTAAATACAAAAc                        >  2:840548/1‑140 (MQ=255)

                                                                                                                   gCTTGTAGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTAAATACAAAACGTAACGc                 <  1:313407/140‑1 (MQ=255)

                                                                                                                         aGCCAGTCGCGGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTAAATACAAAACGTAACGCCTGAtt           >  2:392831/1‑140 (MQ=255)

                                                                                                                                 gcgGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCgg   <  2:383625/140‑1 (MQ=255)

                                                                                                                                 gcgGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGAAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCgg   <  2:728638/140‑1 (MQ=255)

                                                                                                                                  cgGATTTTCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGc  >  2:414864/1‑140 (MQ=255)

GGCATCGCGCACCTGGGCGGCCTCCTGGCGGCTGCGCACCAGCACACTGATGTCCGAAGCACGCACCGGACGCGCGTCGTCGCCGTTCATCAGCAACGCTTCGCCCCGCTGTCCGGCTTGTAGCCAGTCGCGGATTTGCGCAGCACATACCTGCGCCATGGTACTTTGATAATCGCCAACGCCGCAGCTTTCGCCTTCCATCAGCCACATTTTCATCGCAGGCTGTGTTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGC  >  NC_000913/2954294‑2954563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A