Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,165	C→A	G98W (GGG→TGG)	yggP ←	putative zinc‑binding dehydrogenase YggP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,165	0	C	A	100.0%	116.8 / NA	34	G98W (GGG→TGG)	yggP	putative zinc‑binding dehydrogenase YggP
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (18/16); total (18/16)

CCGCAGCAAAATAAGAGCCGTGATACGGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTT  >  NC_000913/3077033‑3077300

ccgcAGCAAAATAAGAGCCGTGATACGGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGcc                                                                                                                                  <  1:154477/140‑1 (MQ=255)

     gcaAAATAAGAGCCGTGATACGGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTg                                                                                                                             <  2:327854/140‑1 (MQ=255)

      caAAATAAGAGCCGTGATACGGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGc                                                                                                                            >  1:248901/1‑140 (MQ=255)

                   gTGATACGGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCt                                                                                                               >  2:415297/1‑140 (MQ=255)

                    tGATACGGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTg                                                                                                              <  1:294986/140‑1 (MQ=255)

                      aTACGGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGc                                                                                                            <  1:209978/140‑1 (MQ=255)

                        aCGGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCt                                                                                                          <  2:381402/140‑1 (MQ=255)

                                    aGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTg                                                                                              >  2:381597/1‑140 (MQ=255)

                                     gCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGc                                                                                             >  2:446500/1‑140 (MQ=255)

                                      cccAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGcc                                                                                            >  1:354036/1‑140 (MQ=255)

                                           aTTAATGGAGATTTCAGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTga                                                                                       <  2:230858/140‑1 (MQ=255)

                                                tGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGAttt                                                                                  >  2:486507/1‑140 (MQ=255)

                                                tGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGAttt                                                                                  >  1:7316/1‑140 (MQ=255)

                                                     aTTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTAcc                                                                             <  2:450454/140‑1 (MQ=255)

                                                     aTTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTAcc                                                                             <  1:500556/140‑1 (MQ=255)

                                                           ggAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTc                                                                       <  2:174154/140‑1 (MQ=255)

                                                                     tataAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAcc                                                             <  1:381597/140‑1 (MQ=255)

                                                                     tataAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAcc                                                             <  2:505802/140‑1 (MQ=255)

                                                                     aataAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGTTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTCCCCACTTCGACAATAAcc                                                             <  2:231858/139‑1 (MQ=255)

                                                                      ataAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAccc                                                            <  2:7316/140‑1 (MQ=255)

                                                                      ataAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAAccc                                                            >  1:158253/1‑140 (MQ=255)

                                                                           tGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGca                                                       >  1:275779/1‑140 (MQ=255)

                                                                            ggCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGATTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGcac                                                      >  2:7047/1‑140 (MQ=255)

                                                                                  gccgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCAt                                                >  1:340769/1‑140 (MQ=255)

                                                                                    cgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGc                                              >  1:283285/1‑140 (MQ=255)

                                                                                     gccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGcc                                             <  2:323274/140‑1 (MQ=255)

                                                                                     gccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGcc                                             <  2:67882/140‑1 (MQ=255)

                                                                                                        tAGCCCGCTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGAtt                          >  1:168221/1‑140 (MQ=255)

                                                                                                               cTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTaaa                   >  1:386542/1‑140 (MQ=255)

                                                                                                               cTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTaaa                   >  2:549762/1‑140 (MQ=255)

                                                                                                               cTGAATATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTaaa                   >  2:591869/1‑140 (MQ=255)

                                                                                                                  aaTATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAAtcg                <  1:572440/140‑1 (MQ=255)

                                                                                                                  aaTATCCGCTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAAtcg                >  2:577098/1‑140 (MQ=255)

                                                                                                                          cTTGGTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTAc        >  2:460240/1‑140 (MQ=255)

                                                                                                                             ggTAACCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTAcgcg     <  2:158253/140‑1 (MQ=255)

                                                                                                                                aaCCACATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGttt  <  1:591869/140‑1 (MQ=255)

CCGCAGCAAAATAAGAGCCGTGATACGGTAATACGCAGCCCAAATTAATGGCGATTTCGGGAATAATCATATAAGTGGCATTGCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGACCGGATGATTTTCTAAATCGTCGGGTACGCGTTT  >  NC_000913/3077033‑3077300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A