Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,200,146	G→T	R274S (CGT→AGT)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,200,146	0	G	T	100.0%	99.9 / NA	29	R274S (CGT→AGT)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (15/14); total (15/14)

CAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTC  >  NC_000913/3200015‑3200278

caaccaTTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACaa                                                                                                                              <  1:12392/140‑1 (MQ=255)

     aTTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATacca                                                                                                                         >  2:534116/1‑140 (MQ=255)

      ttCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATaccac                                                                                                                        <  2:91350/140‑1 (MQ=255)

          gTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGaa                                                                                                                    >  2:573146/1‑140 (MQ=255)

                gCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCAt                                                                                                              <  2:438060/140‑1 (MQ=255)

                gCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCAt                                                                                                              <  1:351676/140‑1 (MQ=255)

                         gCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATg                                                                                                     >  2:357705/1‑140 (MQ=255)

                              tGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGa                                                                                                <  2:50404/140‑1 (MQ=255)

                               gCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGAc                                                                                               <  1:402951/140‑1 (MQ=255)

                                          agagtagaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATgg                                                                                    <  2:128560/140‑1 (MQ=255)

                                              gagaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGGCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGTCTgt                                                                                >  2:113117/2‑139 (MQ=255)

                                               agaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGcc                                                                               <  1:598311/140‑1 (MQ=255)

                                               agaCCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGcc                                                                               <  2:45070/140‑1 (MQ=255)

                                                     ccgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCAcc                                                                         <  1:359190/140‑1 (MQ=255)

                                                      cgTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCt                                                                        >  2:597574/1‑140 (MQ=255)

                                                                      gCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATc                                                        >  1:61004/1‑140 (MQ=255)

                                                                               aTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgct                                               >  2:83669/1‑139 (MQ=255)

                                                                               aTGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCcgcg                                               <  2:57637/140‑1 (MQ=255)

                                                                                 ggTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTa                                             >  2:343391/1‑140 (MQ=255)

                                                                                      ccTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCcac                                        >  1:267386/1‑140 (MQ=255)

                                                                                        tCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAg                                      <  1:470001/140‑1 (MQ=255)

                                                                                          gCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGtt                                    <  1:343391/140‑1 (MQ=255)

                                                                                                      cagATCACGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACt                        >  1:32281/1‑140 (MQ=255)

                                                                                                            aCGTAAGTGAGTGCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCAtt                  <  1:326825/140‑1 (MQ=255)

                                                                                                                   tgagtgCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTaa           >  1:267040/1‑140 (MQ=255)

                                                                                                                     agtgCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACg         >  1:573532/1‑140 (MQ=255)

                                                                                                                     agtgCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACg         >  1:15913/1‑140 (MQ=255)

                                                                                                                       tgCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGcc       >  2:42745/1‑140 (MQ=255)

                                                                                                                       tgCTCGCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGcc       >  1:515519/1‑140 (MQ=255)

                                                                                                                            gCTTTACTCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTc  >  2:274419/1‑140 (MQ=255)

CAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCAGATCACGTAAGTGAGTGCTCGCTTTACGCGGCACAATACCACCGAACAGCATCAGCGTATGACGGACCAGGCTAATGGCTGCCAGCACCTGTTCTTTCGCCGCATCGTTGCCGCGTACCCACAGTTCTTCATGATACTGCCATTGCGCTAACGCCAGCTC  >  NC_000913/3200015‑3200278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A