Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,259,798	G→T	R105R (CGG→AGG)	tdcF ←	putative enamine/imine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,259,798	0	G	T	100.0%	142.7 / NA	41	R105R (CGG→AGG)	tdcF	putative enamine/imine deaminase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (13/28); total (13/28)

TGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGATATCGCCCACGCTCAGCCCGGCAGCAACCACGA  >  NC_000913/3259666‑3259932

tGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGa                                                                                                                                 <  1:239518/140‑1 (MQ=255)

  ccGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCTGATa                                                                                                                               >  2:586834/1‑140 (MQ=255)

         gTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGcc                                                                                                                        <  2:276501/140‑1 (MQ=255)

             aGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGAt                                                                                                                    <  2:184309/140‑1 (MQ=255)

              gTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATg                                                                                                                   <  1:550937/140‑1 (MQ=255)

                    cGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATc                                                                                                             >  1:464050/1‑140 (MQ=255)

                       aaCGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCgaa                                                                                                          <  1:442515/140‑1 (MQ=255)

                         cGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCgaaga                                                                                                        <  1:561080/140‑1 (MQ=255)

                          gACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCgaagaa                                                                                                       <  2:68964/140‑1 (MQ=255)

                          gACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCgaagaa                                                                                                       <  1:15468/140‑1 (MQ=255)

                          gACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCgaagaa                                                                                                       <  2:257017/140‑1 (MQ=255)

                          gACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCgaagaa                                                                                                       <  1:391086/140‑1 (MQ=255)

                          gACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCgaagaa                                                                                                       <  1:592549/140‑1 (MQ=255)

                           aCCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAAc                                                                                                      <  1:117087/140‑1 (MQ=255)

                           aCCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAAc                                                                                                      <  2:175804/140‑1 (MQ=255)

                           aCCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAAc                                                                                                      <  1:542585/140‑1 (MQ=255)

                                 cGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTta                                                                                                >  1:407334/1‑140 (MQ=255)

                                     cGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGa                                                                                            <  1:138460/140‑1 (MQ=255)

                                      gTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGAc                                                                                           <  2:164602/140‑1 (MQ=255)

                                       tCCCGCTCACGATGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACt                                                                                          >  2:84080/1‑140 (MQ=255)

                                               aCGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGAt                                                                                  <  2:377967/140‑1 (MQ=255)

                                                    gCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGAtggtgg                                                                             <  2:342443/140‑1 (MQ=255)

                                                         aCGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCaaaa                                                                        >  2:163338/1‑140 (MQ=255)

                                                                     cTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCg                                                            <  1:188293/140‑1 (MQ=255)

                                                                         gATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGa                                                        >  2:586649/1‑140 (MQ=255)

                                                                           tGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATa                                                      >  2:91090/1‑140 (MQ=255)

                                                                           tGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATa                                                      >  2:360537/1‑140 (MQ=255)

                                                                             gCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATaaa                                                    >  2:260254/1‑140 (MQ=255)

                                                                                  aaTTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACgg                                               <  1:111091/140‑1 (MQ=255)

                                                                                      tCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCAt                                           <  2:313030/140‑1 (MQ=255)

                                                                                      tCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCAt                                           <  2:441642/140‑1 (MQ=255)

                                                                                      tCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCAt                                           <  1:360537/140‑1 (MQ=255)

                                                                                            ttCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTtgat                                     >  1:155345/1‑140 (MQ=255)

                                                                                                 aTCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGatat                                <  2:594411/140‑1 (MQ=255)

                                                                                                 aTCTTTCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGatat                                <  2:87621/140‑1 (MQ=255)

                                                                                                     ttCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGATATCGcc                            <  2:541450/140‑1 (MQ=255)

                                                                                                      tCGGCAAACGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGATATCGccc                           >  1:585986/1‑140 (MQ=255)

                                                                                                            aaCGCGCGACCTGCACACAGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGATATCGCCCACGCTc                     >  2:403761/1‑140 (MQ=255)

                                                                                                                     acTGCACACAGCTCCTGGTCGGATAGGTCGTCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGATATCGCCCACGCTCAGCCCGgca            <  2:464050/139‑1 (MQ=255)

                                                                                                                              aGCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGATATCGCCCACGCTCAGCCCGGCAGCAACCACg   <  2:123615/140‑1 (MQ=255)

                                                                                                                               gCTCCTGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGATATCGCCCACGCTCAGCCCGGCAGCAACCACGa  >  2:248516/1‑140 (MQ=255)

TGCCGTTCAGTGAAGTGGAGCGGAACGACCTTACGACCGTCCCGCTCACGAGGCTTTACGCACTACGTACTGCGATGGCTTCAATTTCCAGCTTCACATCTTTCGGCAAACGCGCGACCTGCACACAGCTCCGGGTCGGATAGGTCGCCTGATGCTCATCGAAGAACTGCTTATAGACTTCGTTGATGGTGGCAAAATCATTCAGATCGGTGATAAACACGGTCATCTTGATGATATCGCCCACGCTCAGCCCGGCAGCAACCACGA  >  NC_000913/3259666‑3259932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A