Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,261,774	G→T	G215G (GGC→GGA)	tdcE ←	2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,261,774	0	G	T	100.0%	210.1 / NA	60	G215G (GGC→GGA)	tdcE	2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/38); total (22/38)

TGCACCGCTTCCTGCGCATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCGCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGG  >  NC_000913/3261637‑3261907

tGCACCGCTTCCTGCGCATTCTTCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTcc                                                                                                                                     >  2:614273/1‑140 (MQ=255)

 gCACCGCTTCCTGCGCATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCt                                                                                                                                    >  1:828185/1‑140 (MQ=255)

      gCTTCCTGCGCATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTc                                                                                                                               <  2:218326/140‑1 (MQ=255)

        ttCCTGCGCATTCTGGGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCa                                                                                                                             <  1:25185/140‑1 (MQ=255)

          ccTGCGCATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGTCGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGa                                                                                                                           >  2:440033/1‑140 (MQ=255)

          ccTGCGCATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGa                                                                                                                           <  2:615409/140‑1 (MQ=255)

          ccTGCGCATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGa                                                                                                                           >  1:646046/1‑140 (MQ=255)

          ccTGCGCATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATACTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGa                                                                                                                           <  1:693158/140‑1 (MQ=255)

           cTGCGCATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGAc                                                                                                                          <  1:144434/140‑1 (MQ=255)

             gcgcATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACga                                                                                                                        <  2:36564/140‑1 (MQ=255)

             gcgcATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACga                                                                                                                        <  2:37163/140‑1 (MQ=255)

             gcgcATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACga                                                                                                                        >  1:274442/1‑140 (MQ=255)

                   tCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTgg                                                                                                                  >  2:227244/1‑140 (MQ=255)

                            ggCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCa                                                                                                         >  2:587966/1‑140 (MQ=255)

                                    atatCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAg                                                                                                 <  1:614273/140‑1 (MQ=255)

                                      atCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGtt                                                                                               <  2:707365/140‑1 (MQ=255)

                                      atCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGtt                                                                                               <  2:415937/140‑1 (MQ=255)

                                         aaaGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTcgc                                                                                            <  1:285203/140‑1 (MQ=255)

                                           aGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGt                                                                                          >  1:117450/1‑140 (MQ=255)

                                             ccATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTc                                                                                        <  2:548350/140‑1 (MQ=255)

                                              aatatTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCa                                                                                       <  2:646046/139‑1 (MQ=255)

                                               atatTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCAc                                                                                      >  2:479747/1‑140 (MQ=255)

                                                tatTTCGCCGCCATTTCCTGAATCTACAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACg                                                                                     <  2:455861/140‑1 (MQ=255)

                                                tatTTCGCCGCCATTTCCTGAATCTACAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACg                                                                                     >  1:455861/1‑140 (MQ=255)

                                                  tttCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTa                                                                                   <  1:349893/140‑1 (MQ=255)

                                                  tttCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTa                                                                                   <  2:171386/140‑1 (MQ=255)

                                                  tttCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTa                                                                                   <  2:836304/140‑1 (MQ=255)

                                                       ccgccATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGa                                                                              >  2:832717/1‑140 (MQ=255)

                                                        cgccATTTCCTGAATCTGCAACATCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGAt                                                                             <  2:722678/140‑1 (MQ=255)

                                                          ccATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATaa                                                                           >  2:418103/1‑140 (MQ=255)

                                                           cATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAAc                                                                          <  1:414643/140‑1 (MQ=255)

                                                           cATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAAc                                                                          <  1:337859/140‑1 (MQ=255)

                                                           cATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAAc                                                                          <  1:780230/140‑1 (MQ=255)

                                                           aaTTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAAc                                                                          <  2:675452/139‑1 (MQ=255)

                                                             tttCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGTCGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTg                                                                        <  1:440033/140‑1 (MQ=255)

                                                              ttCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGa                                                                       <  2:614209/140‑1 (MQ=255)

                                                                ccTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATg                                                                     <  1:227244/140‑1 (MQ=255)

                                                                   aaaTCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGAGTGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCa                                                                  <  2:665358/139‑1 (MQ=255)

                                                                         gCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAgc                                                            >  1:76099/1‑140 (MQ=255)

                                                                               gcgcATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTAcg                                                      <  1:479747/140‑1 (MQ=255)

                                                                                cgcATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTAcgc                                                     >  2:68515/1‑140 (MQ=255)

                                                                                cgcATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTAcgc                                                     >  2:538228/1‑140 (MQ=255)

                                                                                    tGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACgcggcg                                                 >  2:216693/1‑140 (MQ=255)

                                                                                         aTGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGt                                            <  2:607514/140‑1 (MQ=255)

                                                                                         aTGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGt                                            <  2:549650/140‑1 (MQ=255)

                                                                                         aTGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGt                                            <  1:68515/140‑1 (MQ=255)

                                                                                                    gCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATg                                 <  1:752573/140‑1 (MQ=255)

                                                                                                      tcctcACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATgcg                               >  1:650088/1‑140 (MQ=255)

                                                                                                         tcACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGccc                            <  1:587966/140‑1 (MQ=255)

                                                                                                          cACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCa                           <  2:117450/140‑1 (MQ=255)

                                                                                                          cACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCa                           <  2:12386/140‑1 (MQ=255)

                                                                                                          cACGCAGACGGATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCATTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGTGATAGTCACCGATAATGCGCCCa                           <  2:232962/140‑1 (MQ=255)

                                                                                                                    gATGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGc                 >  1:3465/1‑140 (MQ=255)

                                                                                                                     aTGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGcc                <  2:296326/140‑1 (MQ=255)

                                                                                                                     aTGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGcc                <  2:828185/140‑1 (MQ=255)

                                                                                                                     aTGGTGGCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGcc                >  1:400551/1‑140 (MQ=255)

                                                                                                                           gCTTCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTgg          >  1:671858/1‑140 (MQ=255)

                                                                                                                            tttCCAGATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCATATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGt         <  2:274442/139‑1 (MQ=255)

                                                                                                                                  gATCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCg   >  1:756679/1‑140 (MQ=255)

                                                                                                                                   aTCCTCTCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCgg  >  2:525107/1‑140 (MQ=255)

TGCACCGCTTCCTGCGCATTCTGCGCCGGGCGAGAGATATCAAAGCCATATTTCGCCGCCATTTCCTGAATCTGCAACAGCGCATGACGATGCTCTGCCAGCTCCTCACGCAGACGGATGGTGGCTTCCAGATCCTCGCCTTTTTCCAGACGAGACTGGAGATCGGCAAATTGCAGTTCGCGTTCACGTACCAGATAACTGATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGG  >  NC_000913/3261637‑3261907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A