Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F48 I1 R1
|
90 |
35.3 |
1199032 |
98.5% |
1181046 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,445,101 |
G→T |
N43K (AAC→AAA) |
rpsE ← |
30S ribosomal subunit protein S5 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,445,101 | 0 | G | T | 96.7%
| 99.0
/ ‑3.2
| 30 | N43K (AAC→AAA) | rpsE | 30S ribosomal subunit protein S5 |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (16/13); total (17/13) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 |
AACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTT > NC_000913/3444966‑3445232
|
aaCACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTAc < 2:558079/140‑1 (MQ=255)
cacCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCa > 1:525492/1‑140 (MQ=255)
tttAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGc < 1:219549/140‑1 (MQ=255)
gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACat > 2:164043/1‑139 (MQ=255)
gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAg > 1:578974/1‑140 (MQ=255)
gtgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAg > 2:437404/1‑140 (MQ=255)
tgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGt < 1:472640/140‑1 (MQ=255)
ttGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCa > 1:485993/1‑140 (MQ=255)
gCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCaga < 1:347123/140‑1 (MQ=255)
tGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCtgtg > 1:312383/1‑140 (MQ=255)
ggCCGGTATTCAGCCCGCGGTTAGTCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAGAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCtgtg > 2:430957/2‑140 (MQ=255)
gCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGa < 2:464345/140‑1 (MQ=255)
caggTTATTCAGCTCGACGTTCATCATATTGCCACGGGCTTTTTCCATCGCTTTCTCGATCGCTGCTGGAACTTCACCCGCTTTACCGTAACCAAAACCAACGCAACCTTTACCATCGCCAACTCCAGTCAGAGCTGTGa < 2:207282/137‑1 (MQ=255)
ccGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGaa > 1:471407/1‑140 (MQ=255)
cgcgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATAc > 2:79798/1‑140 (MQ=255)
cgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATActg > 1:207282/1‑138 (MQ=255)
aTCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGGGAAGGAGAAAATACGACCACCttt > 1:328671/1‑140 (MQ=255)
tCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTa < 1:299321/140‑1 (MQ=255)
cATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCGCCTTTaa < 1:430957/140‑1 (MQ=255)
cATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCACCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTaa < 2:54724/140‑1 (MQ=255)
atTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACgg > 2:523632/1‑140 (MQ=255)
tGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGtt > 1:249158/1‑140 (MQ=255)
cGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTAGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGtttt > 1:394220/1‑140 (MQ=255)
aCGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAg > 2:320355/1‑140 (MQ=255)
cTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAcg < 1:79798/140‑1 (MQ=255)
cTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCGCCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCa < 2:525492/140‑1 (MQ=255)
ctgctgGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTcc < 1:149919/140‑1 (MQ=255)
tgctgGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCt > 1:166174/1‑140 (MQ=255)
attCACGCGCTTTACCGTAACCAAAACCAACGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCg < 2:191506/139‑1 (MQ=255)
ccaaaaccaaCGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAtgtg > 1:527659/1‑140 (MQ=255)
aaaaccaaCGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAg < 2:578974/140‑1 (MQ=255)
aaccaaCGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGcc > 2:472751/1‑140 (MQ=255)
aCGCGACCTTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCtt < 1:471690/140‑1 (MQ=255)
|
AACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCATCTT > NC_000913/3444966‑3445232
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
