Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,520,495	G→T	A70E (GCA→GAA)	hofQ ←	DNA utilization protein HofQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,520,495	0	G	T	100.0%	121.9 / NA	36	A70E (GCA→GAA)	hofQ	DNA utilization protein HofQ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (21/15); total (21/15)

GCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGC  >  NC_000913/3520358‑3520629

gCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTcc                                                                                                                                      <  1:373245/140‑1 (MQ=255)

 cAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCt                                                                                                                                     <  2:134003/140‑1 (MQ=255)

           tGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAggg                                                                                                                           <  1:465973/140‑1 (MQ=255)

              gcCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAAc                                                                                                                        <  1:514429/140‑1 (MQ=255)

               cccGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACa                                                                                                                       <  2:597479/140‑1 (MQ=255)

                      cTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTa                                                                                                                <  2:185749/140‑1 (MQ=255)

                        gCTCCGCCTCCTGGCGGGCGATACTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGa                                                                                                              <  2:377623/140‑1 (MQ=255)

                                tcctGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGa                                                                                                      >  2:509884/1‑140 (MQ=255)

                                tcctGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGa                                                                                                      <  1:509884/140‑1 (MQ=255)

                                                     ttCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGTCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTgg                                                                                 >  1:97069/1‑140 (MQ=255)

                                                         gCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGTCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGac                                                                             >  2:52876/1‑140 (MQ=255)

                                                          ccAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGaca                                                                            >  1:459730/1‑140 (MQ=255)

                                                          ccAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGaca                                                                            >  1:39399/1‑140 (MQ=255)

                                                                  tGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAgg                                                                    >  1:436541/1‑140 (MQ=255)

                                                                       tGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCaa                                                               >  2:490092/1‑140 (MQ=255)

                                                                         cACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACt                                                             >  2:127641/1‑140 (MQ=255)

                                                                         cACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACt                                                             >  2:431644/1‑140 (MQ=255)

                                                                           cTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTtc                                                           <  1:139195/140‑1 (MQ=255)

                                                                              agagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                                        >  1:355990/1‑140 (MQ=255)

                                                                              agagaATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTcc                                                        >  2:23329/1‑140 (MQ=255)

                                                                                         ccTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagccag                                             >  1:467524/1‑140 (MQ=255)

                                                                                          cTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGGTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTcagccagc                                            >  1:466155/1‑140 (MQ=255)

                                                                                                 ccGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCa                                     <  1:519974/140‑1 (MQ=255)

                                                                                                  cgcgTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCaa                                    <  1:507077/140‑1 (MQ=255)

                                                                                                      ttATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACAcc                                <  2:405959/140‑1 (MQ=255)

                                                                                                          cAGTCCGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAg                            <  2:114034/140‑1 (MQ=255)

                                                                                                               cGGCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTAcc                       >  2:343107/1‑140 (MQ=255)

                                                                                                                ggCGCTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACGCCTGAGCTACCg                      <  2:466155/140‑1 (MQ=255)

                                                                                                                 gcgcTTTTCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCgg                     >  2:461025/1‑140 (MQ=255)

                                                                                                                      tttCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGt                <  2:377797/140‑1 (MQ=255)

                                                                                                                      tttCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGt                <  1:375618/140‑1 (MQ=255)

                                                                                                                       ttCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTc               >  2:329582/1‑140 (MQ=255)

                                                                                                                       ttCACTACAGTTTGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTc               >  2:459172/1‑140 (MQ=255)

                                                                                                                                   tGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAg   >  2:243128/1‑140 (MQ=255)

                                                                                                                                   tGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAg   >  1:405765/1‑140 (MQ=255)

                                                                                                                                   tGTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAg   >  1:1723/1‑140 (MQ=255)

                                                                                                                                    gTAGTTCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGc  >  1:531647/1‑140 (MQ=255)

GCAGATTTGCCTGCGCCCGCGCCTGCTCCGCCTCCTGGCGGGCGATATTGTTATTCTGCCAGGCAATGGAATGCACTGAGAGAATGTTGCCTTCCTGCCGCGTTATCAGTCCGGCGCTTTTCACTACAGTTTGTAGTGCCTGCTTCCAGGGAACATCTGTTAGATGTAACGACACCGTACCGCTGACGTCTGGCGACACGACCAGGTTCAACTTCTCCTGTTCAGCCAGCGCCTGCAACACCTGAGCTACCGGAACGTCATCCACCATCAGC  >  NC_000913/3520358‑3520629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A