Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,000,241	G→T	intergenic (+96/+51)	uvrD → / ← yigE	ssDNA translocase and dsDNA helicase ‑ DNA helicase II/DUF2233 domain‑containing protein YigE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,000,241	0	G	T	100.0%	125.2 / NA	36	intergenic (+96/+51)	uvrD/yigE	ssDNA translocase and dsDNA helicase ‑ DNA helicase II/DUF2233 domain‑containing protein YigE
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/20); total (16/20)

GGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATG  >  NC_000913/4000108‑4000372

ggCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATACATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCAt                                                                                                                               <  1:576315/140‑1 (MQ=255)

ggCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCAt                                                                                                                               >  1:61732/1‑140 (MQ=255)

  cTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATcc                                                                                                                             >  2:410368/1‑140 (MQ=255)

  cTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATcc                                                                                                                             >  1:381647/1‑140 (MQ=255)

  cTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATcc                                                                                                                             >  2:267384/1‑140 (MQ=255)

  cTGGTGGCGGCATACGCCCGGCTGGAGTCGGGGTAACGTTGCCGGATGCTGTGCTGCGCACCTTATTTGGTCGAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGTCCGGATAGGGCGTTTACTCCGCATcc                                                                                                                             >  2:245966/1‑140 (MQ=255)

       ggcggcATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCAt                                                                                                                        <  2:180170/140‑1 (MQ=255)

           gcATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTgc                                                                                                                    <  1:267384/140‑1 (MQ=255)

              tACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGcc                                                                                                                 <  2:179038/140‑1 (MQ=255)

                          gAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATc                                                                                                     >  2:298404/1‑140 (MQ=255)

                                       gTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTa                                                                                        <  1:248295/140‑1 (MQ=255)

                                           ccGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGt                                                                                    >  2:552427/1‑140 (MQ=255)

                                                            cACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCg                                                                   >  2:232178/1‑140 (MQ=255)

                                                               cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACg                                                                >  2:86518/1‑140 (MQ=255)

                                                               cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACg                                                                >  2:141586/1‑140 (MQ=255)

                                                                         cTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATgg                                                      >  1:202727/1‑140 (MQ=255)

                                                                          tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGt                                                     <  2:61732/140‑1 (MQ=255)

                                                                              aaaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACa                                                 <  1:472274/140‑1 (MQ=255)

                                                                                    ttCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGa                                           <  2:202727/140‑1 (MQ=255)

                                                                                       aGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAg                                        <  1:134983/140‑1 (MQ=255)

                                                                                         aTTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCg                                      >  1:365453/1‑140 (MQ=255)

                                                                                         aTTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCg                                      >  2:127110/1‑140 (MQ=255)

                                                                                          atCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGt                                     <  2:44706/139‑1 (MQ=255)

                                                                                               tAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCa                                <  1:127110/140‑1 (MQ=255)

                                                                                                   ttGCAACGTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACggg                            >  2:364484/1‑140 (MQ=255)

                                                                                                          gTCATGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGccc                     <  1:145848/140‑1 (MQ=255)

                                                                                                              tGTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGcc                 <  1:103056/140‑1 (MQ=255)

                                                                                                              agTAAGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGcc                 <  2:59954/139‑1 (MQ=255)

                                                                                                               gTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCt                <  2:10388/140‑1 (MQ=255)

                                                                                                               gTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCt                <  1:403758/140‑1 (MQ=255)

                                                                                                               gTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCt                <  2:381647/140‑1 (MQ=255)

                                                                                                               gTAGGCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCt                <  1:279561/140‑1 (MQ=255)

                                                                                                                   gCCGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCa            >  1:289991/1‑140 (MQ=255)

                                                                                                                    ccGGATAGGGCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCat           <  1:187487/140‑1 (MQ=255)

                                                                                                                        aTAGGGCGTTTACTCCGCATCTGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTATCCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTAGCCACGGGATTGCCCCGCCTTTCATATAc       <  2:559250/140‑1 (MQ=255)

                                                                                                                             gCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtg  >  1:264212/1‑140 (MQ=255)

                                                                                                                             gCGTTTACTCCGCATCCGGCATCTGCGCCATCTTCAGAATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtg  >  1:390085/1‑140 (MQ=255)

GGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATG  >  NC_000913/4000108‑4000372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A