Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F48 I1 R1	115	87.5	3003888	99.1%	2976853	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,001,946	C→A	A174E (GCG→GAG)	corA →	Ni(2(+))/Co(2(+))/Mg(2(+)) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,001,946	0	C	A	98.7%	268.5 / ‑3.1	77	A174E (GCG→GAG)	corA	Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (35/41); total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

ACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAGGCGCGTGAAATC  >  NC_000913/4001808‑4002082

aCGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGgcg                                                                                                                                         >  2:828877/1‑140 (MQ=255)

   ccTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGctc                                                                                                                                      >  1:803014/1‑140 (MQ=255)

   ccTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGctc                                                                                                                                      >  2:772150/1‑140 (MQ=255)

    cTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGctct                                                                                                                                     <  2:805819/140‑1 (MQ=255)

             gctgctGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTgg                                                                                                                            <  1:1021370/140‑1 (MQ=255)

             gctgctGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTgg                                                                                                                            <  2:1166318/140‑1 (MQ=255)

              ctgctgGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGc                                                                                                                           >  2:1075463/1‑140 (MQ=255)

               tgctgGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGAGCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCg                                                                                                                          <  2:571565/140‑1 (MQ=255)

               tgctgGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCg                                                                                                                          <  1:146953/140‑1 (MQ=255)

                gctgGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCgg                                                                                                                         <  2:232425/140‑1 (MQ=255)

                 ctgGATCTGTTCGAAACCAAAATCGAACAGTTGGTAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGa                                                                                                                        >  2:1083293/1‑140 (MQ=255)

                  tgGATCTGTTCGTAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGaa                                                                                                                       <  1:1482483/140‑1 (MQ=255)

                  tgGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGaa                                                                                                                       <  2:747588/140‑1 (MQ=255)

                  tgGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGaa                                                                                                                       <  2:742366/140‑1 (MQ=255)

                   ggATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAAc                                                                                                                      <  2:1276674/140‑1 (MQ=255)

                         gTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAg                                                                                                                >  1:1202000/1‑140 (MQ=255)

                            cGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGata                                                                                                             >  1:818605/1‑140 (MQ=255)

                             gAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGatat                                                                                                            <  2:405232/140‑1 (MQ=255)

                               aaCCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCg                                                                                                          >  1:177452/1‑140 (MQ=255)

                                    aaaTCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGATCTCCACTCTGGCGGAACTGGAAGATATCGGCTgg                                                                                                     >  2:1375311/1‑140 (MQ=255)

                                             aGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGc                                                                                            <  1:828877/140‑1 (MQ=255)

                                             aGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGc                                                                                            <  2:878914/140‑1 (MQ=255)

                                             aGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGc                                                                                            <  1:605589/140‑1 (MQ=255)

                                                 ggCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCtgt                                                                                        >  2:163946/1‑140 (MQ=255)

                                                 ggCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCtgt                                                                                        <  1:1419786/140‑1 (MQ=255)

                                                 ggCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCtgt                                                                                        >  1:723666/1‑140 (MQ=255)

                                                   cAGATGAAATTGGAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTgtgt                                                                                      <  1:336852/140‑1 (MQ=255)

                                                     gATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCt                                                                                    <  1:1346351/140‑1 (MQ=255)

                                                     gATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCt                                                                                    <  2:1131836/140‑1 (MQ=255)

                                                            ttGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGAt                                                                             >  1:1370722/1‑140 (MQ=255)

                                                                aaaTATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATAccc                                                                         <  2:1148331/140‑1 (MQ=255)

                                                                   tatCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCt                                                                                    >  2:635703/1‑126 (MQ=255)

                                                                   tatCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCt                                                                                    <  1:635703/126‑1 (MQ=255)

                                                                        ataGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAgcgcgcg                                                                 <  2:688491/140‑1 (MQ=255)

                                                                        ataGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAgcgcgcg                                                                 <  2:551412/140‑1 (MQ=255)

                                                                          aGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCt                                                               <  1:866524/140‑1 (MQ=255)

                                                                          aGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCt                                                               <  1:235669/140‑1 (MQ=255)

                                                                          aGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCt                                                               <  2:1202000/140‑1 (MQ=255)

                                                                            cGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCa                                                             <  1:163946/140‑1 (MQ=255)

                                                                            cGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCa                                                             <  2:586478/140‑1 (MQ=255)

                                                                            cGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCa                                                             <  2:177452/140‑1 (MQ=255)

                                                                              aCCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGTTGGATACCCAGCGCGCGCTCAAc                                                           >  2:844305/1‑140 (MQ=255)

                                                                              aCCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAAc                                                           >  2:235792/1‑140 (MQ=255)

                                                                              aCCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAAc                                                           >  1:703066/1‑140 (MQ=255)

                                                                              aCCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGGCTGATGGATACCCAGCGCGCGCTCAAc                                                           >  2:951103/1‑140 (MQ=255)

                                                                               ccTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACt                                                          >  1:902000/1‑140 (MQ=255)

                                                                               ccTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACt                                                          <  1:607815/140‑1 (MQ=255)

                                                                               ccTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACt                                                          >  2:1106837/1‑140 (MQ=255)

                                                                                 tGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTc                                                        <  1:23317/140‑1 (MQ=255)

                                                                                         ttGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTTGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCg                                                >  1:1176583/1‑140 (MQ=255)

                                                                                         ttGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCg                                                >  2:240669/1‑140 (MQ=255)

                                                                                         ttGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCg                                                >  1:997191/1‑140 (MQ=255)

                                                                                         ttGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCg                                                >  2:506866/1‑140 (MQ=255)

                                                                                         ttGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCg                                                >  1:130578/1‑140 (MQ=255)

                                                                                         ttGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAACGCGCGCTCAACTTCCTTGTGct                                                >  2:460745/1‑139 (MQ=255)

                                                                                            aGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTaa                                             <  2:902000/140‑1 (MQ=255)

                                                                                             gCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTaaa                                            <  1:610607/140‑1 (MQ=255)

                                                                                              ccGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAg                                           >  1:1160191/1‑140 (MQ=255)

                                                                                                gggTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAgcg                                         <  2:997191/140‑1 (MQ=255)

                                                                                                gggTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAgcg                                         <  1:1083293/140‑1 (MQ=255)

                                                                                                gggTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAgcg                                         <  1:240669/140‑1 (MQ=255)

                                                                                                gggTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAgcg                                         >  1:720575/1‑140 (MQ=255)

                                                                                                           gAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGTGCGTTTACCgcg                              >  2:621560/1‑138 (MQ=255)

                                                                                                           gAAGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCggg                              <  1:772150/140‑1 (MQ=255)

                                                                                                             aGGGCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTg                            <  1:1106837/140‑1 (MQ=255)

                                                                                                              gggCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAATGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTgg                           <  2:723666/140‑1 (MQ=255)

                                                                                                               ggCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTggg                          >  1:482460/1‑140 (MQ=255)

                                                                                                               ggCATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTggg                          >  2:970776/1‑140 (MQ=255)

                                                                                                                 cATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCa                        >  2:441285/1‑140 (MQ=255)

                                                                                                                 cATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCa                        >  1:325634/1‑140 (MQ=255)

                                                                                                                 cATCAGGGCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCa                        >  2:42831/1‑140 (MQ=255)

                                                                                                                        gCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAg                 <  2:1370722/140‑1 (MQ=255)

                                                                                                                        gCGATGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAg                 <  2:803014/140‑1 (MQ=255)

                                                                                                                           aTGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAg              >  2:430262/1‑140 (MQ=255)

                                                                                                                            tGAGTACGACGAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAgg             <  1:234243/140‑1 (MQ=255)

                                                                                                                                 acgacgAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAGGCGCGt        >  2:1425697/1‑140 (MQ=255)

                                                                                                                                 acgacgAGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAGGCGCGt        >  1:889709/1‑140 (MQ=255)

                                                                                                                                       aGGAGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAGGCGCGTGAAATc  <  2:671499/140‑1 (MQ=255)

ACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAGGCGCGTGAAATC  >  NC_000913/4001808‑4002082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A