Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,029,849	C→A	A375S (GCT→TCT)	fadB ←	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,029,849	0	C	A	100.0%	110.1 / NA	32	A375S (GCT→TCT)	fadB	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/15); total (17/15)

TGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGTTGATATCTTTCATGACAACCGGCACGCCTTTCCACGCA  >  NC_000913/4029713‑4029976

tGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACTACAATATCCACGCGGTCACATCCGGCGTAGTCGAGCGTTGGGTGGATTCTGGAGATCACgccagacat                                                                                                                              >  2:16010/1‑139 (MQ=255)

tGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGccagacag                                                                                                                              >  1:537408/1‑140 (MQ=255)

     tACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCa                                                                                                                         >  1:210069/1‑140 (MQ=255)

     tACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCa                                                                                                                         >  2:417189/1‑140 (MQ=255)

       cTTTTTGTTCGGTTTCTGCCAGTACTGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGa                                                                                                                       >  2:467936/1‑140 (MQ=255)

       cTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGa                                                                                                                       >  1:382839/1‑140 (MQ=255)

       cTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGa                                                                                                                       >  1:461020/1‑140 (MQ=255)

       cTTTTTGTTCGGTTTCTGCCAGTACGGCTTATTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTTAGa                                                                                                                       >  1:138825/1‑140 (MQ=255)

          tttGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCa                                                                                                                    >  1:461051/1‑140 (MQ=255)

                cGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATc                                                                                                              >  2:580703/1‑140 (MQ=255)

                             tACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCaa                                                                                                 >  1:335309/1‑140 (MQ=255)

                                ggCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAgct                                                                                              <  1:249450/140‑1 (MQ=255)

                                  cTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAgctgc                                                                                            <  2:179171/140‑1 (MQ=255)

                                                 ggTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTc                                                                             >  2:131103/1‑140 (MQ=255)

                                                  gTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTcg                                                                            <  1:467936/140‑1 (MQ=255)

                                                                cTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATg                                                              >  1:361561/1‑140 (MQ=255)

                                                                  tCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGcc                                                            >  1:169147/1‑140 (MQ=255)

                                                                    tACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGa                                                          <  1:580703/140‑1 (MQ=255)

                                                                        acaATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGt                                                      >  2:76321/1‑140 (MQ=255)

                                                                         caATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGtt                                                     <  1:390596/140‑1 (MQ=255)

                                                                         caATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGtt                                                     <  1:383108/140‑1 (MQ=255)

                                                                                 aCGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACtt                                             <  2:210069/140‑1 (MQ=255)

                                                                                   gcgGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGt                                           <  2:169147/140‑1 (MQ=255)

                                                                                    cgGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTc                                          >  2:477727/1‑140 (MQ=255)

                                                                                      gTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTg                                            <  1:420780/136‑1 (MQ=255)

                                                                                      gTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTg                                            >  2:420780/1‑136 (MQ=255)

                                                                                       tCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGtt                                       <  2:335309/140‑1 (MQ=255)

                                                                                       tCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGtt                                       >  1:230127/1‑140 (MQ=255)

                                                                                               ggCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGTTGATATCtt                               >  1:459159/1‑140 (MQ=255)

                                                                                                       cGAGCGTTGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGTTGATATCTTTCATGACa                       <  1:76321/140‑1 (MQ=255)

                                                                                                             ttGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGTTGATATCTTTCATGACAACCGGc                 <  1:539832/140‑1 (MQ=255)

                                                                                                              tGGGTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGTTGATATCTTTCATGACAACCGGCa                <  2:382839/140‑1 (MQ=255)

                                                                                                                ggTGGATTGTGGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGTTGATATCTTTCATGACAACCGGCACg              >  1:323488/1‑140 (MQ=255)

                                                                                                                       tgtgGAGATCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGTTGATATCTTTCATGACAACCGGCACGCCTTTcc       <  2:537408/140‑1 (MQ=255)

                                                                                                                            agaTCACGCCAGACAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGTTGATATCTTTCATGACAACCGGCACGCCTTTCCACGCa  <  2:243386/140‑1 (MQ=255)

TGGCGTACTTTTTGTTCGGTTTCTGCCAGTACGGCTTTTTTCACTTTCGGGTTTTCAACAACCGCTTCTACCACAATATCCACGCGGTCAAATCCGGCGTAGTCGAGCGTTGGGTGGATTGTGGAGATCACGCCAGCCAGTTTCAGACCATCGATCTTGCCGCGCTCAAGCTGCTTGTTCAGCAGTTTCGCGGCTTCGGTCATGCCGAGGGTTAACGACTTGTCGTTGATATCTTTCATGACAACCGGCACGCCTTTCCACGCA  >  NC_000913/4029713‑4029976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A