Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,088,390	C→A	R489L (CGC→CTC)	frvR ←	putative transcriptional regulator FrvR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,088,390	0	C	A	100.0%	169.7 / NA	49	R489L (CGC→CTC)	frvR	putative transcriptional regulator FrvR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (26/23); total (26/23)

CATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGCGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCGGGTAATATGTTGCCAGCTTTCACCGCGTACATTCGAATAATGAAAACTTCCGGGGGCAGAGAAAA  >  NC_000913/4088253‑4088525

cATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAgg                                                                                                                                       >  1:323444/1‑140 (MQ=255)

   ggTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCg                                                                                                                                    >  2:55084/1‑140 (MQ=255)

    gTTAATGACTTCGTTATTCACCTCAACTGTGTGGGCGAGGGTAATTAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCTAGCTATGTGGGATGGCGAGGCGATTAACAATCAGTGTTTCACCTTCGCCTTCGAGTGCgt                                                                                                                                   >  2:846/1‑139 (MQ=255)

    gTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCga                                                                                                                                   >  2:1094/1‑140 (MQ=255)

         tGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAATCGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGatgatg                                                                                                                              >  2:86970/1‑140 (MQ=255)

         tGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGatgatg                                                                                                                              >  2:78967/1‑140 (MQ=255)

         tGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGatgatg                                                                                                                              >  2:144528/1‑140 (MQ=255)

             ttCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGtt                                                                                                                          >  2:585226/1‑140 (MQ=255)

             ttCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGtt                                                                                                                          >  2:250505/1‑140 (MQ=255)

             ttCGTTATTCACCTCAAATGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGGTCCTGTTCGCTCCAGAAATGTGGGATGGCGTGGCGATTAACAATCAGGTTTTCTCCTTCGCCTTCGAGGGCGATGATGCGtt                                                                                                                          >  2:563890/1‑140 (MQ=255)

               cGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTtgt                                                                                                                        >  2:98757/1‑140 (MQ=255)

               cGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTtgt                                                                                                                        >  2:548653/1‑140 (MQ=255)

               cGTTATTCACCTCAACTGGTTGAGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTtgt                                                                                                                        >  2:125248/1‑140 (MQ=255)

                      cACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCAt                                                                                                                 <  2:509177/140‑1 (MQ=255)

                      cACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCAt                                                                                                                 <  1:349571/140‑1 (MQ=255)

                       aCCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATc                                                                                                                <  2:809/140‑1 (MQ=255)

                         cTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATcgg                                                                                                              <  1:123776/140‑1 (MQ=255)

                           cAACTGGTTGAGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATcggcg                                                                                                            <  1:125248/140‑1 (MQ=255)

                            aaCTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATcggcgg                                                                                                           <  1:178993/140‑1 (MQ=255)

                            aaCTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATcggcgg                                                                                                           <  1:224879/140‑1 (MQ=255)

                            aaCTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATcggcgg                                                                                                           <  1:250505/140‑1 (MQ=255)

                            aaCTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATcggcgg                                                                                                           <  1:846/140‑1 (MQ=255)

                              cTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTa                                                                                                         <  2:323444/140‑1 (MQ=255)

                              cTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTa                                                                                                         <  1:98757/140‑1 (MQ=255)

                                 gTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATcggcggcctta                                                                                                      >  1:434307/1‑135 (MQ=255)

                                 gTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCATTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAata                                                                                                      <  1:86970/140‑1 (MQ=255)

                                  ttGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAatat                                                                                                     <  1:78967/140‑1 (MQ=255)

                                   tGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATg                                                                                                    >  1:347955/1‑140 (MQ=255)

                                           ggTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTTATATTGggttttt                                                                                            >  2:196768/1‑137 (MQ=255)

                                                taaAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGTGACt                                                                                       >  2:477299/1‑140 (MQ=255)

                                                taaAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGGCt                                                                                       >  2:148380/1‑140 (MQ=255)

                                                      aTCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATTGTGTTGTGCGGCTAATTgt                                                                                 >  2:352148/1‑139 (MQ=255)

                                                      aTCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAAAATGGGGGTGTGGGGCTAAATgc                                                                                 >  1:299048/1‑140 (MQ=255)

                                                         cACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGca                                                                              <  1:144528/140‑1 (MQ=255)

                                                           cGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTGATATGGGGGTGGGGGACTAATTGCCcaca                                                                            >  1:130957/1‑140 (MQ=255)

                                                                   tCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGcc                                                                    <  1:399397/140‑1 (MQ=255)

                                                                   tCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGcc                                                                    <  1:548653/140‑1 (MQ=255)

                                                                      cTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCggg                                                                 <  1:196768/140‑1 (MQ=255)

                                                                         cTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCGGGTaa                                                              <  1:1094/140‑1 (MQ=255)

                                                                                 tCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCGTGTAATATGTTGc                                                      <  1:55084/140‑1 (MQ=255)

                                                                                   cAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGGTATATGGTGTTGTGCGGCTAATTTGGCTCAAATTTTTCGGGGAATATGttttct                                                    >  2:44764/1‑136 (MQ=255)

                                                                                               aTGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCGGGTAATATGTTGCCAGCTTTCACcgcg                                        <  1:75564/140‑1 (MQ=255)

                                                                                               aTGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCGGGTAATATGTTGCCAGCTTTCACcgcg                                        <  1:381168/140‑1 (MQ=255)

                                                                                               aTGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGGGGTGTGGGGCTTATTTCGCCCAAAATTTCCGGGGAATATGTTTTCCGCTTTTACcgcc                                        >  2:438030/1‑139 (MQ=255)

                                                                                                tGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATTGGGTTGTGCGGCTAAATGCGCCCAAAATTTCCGGGGGATATTTTTCCAGCTTTCACCGCGt                                       >  2:75564/1‑140 (MQ=255)

                                                                                                 ggcgaggcgaTTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCGGGTAATATGTTGCCAGCTTTCACCGCGTa                                      <  2:512177/140‑1 (MQ=255)

                                                                                                  gcgaggcgaTTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTTTGCGACTAATTTTGCACAAAATTTTCGGGGAATATGTTGCCAGCTTTTACCTCGttc                                     >  2:253358/1‑138 (MQ=255)

                                                                                                   cgaggcgaTTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATTGTGTTGTGCGGCTAATTGCGCACAAATTTTCCGGGGAATATGTTGCCAGCTTTCTCCGCGTACa                                    >  2:405955/1‑140 (MQ=255)

                                                                                                      ggcgaTTAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCGGGTAATATGTTGCCAGCTTTCACCGCGTACATTc                                 <  1:352148/140‑1 (MQ=255)

                                                                                                            tAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGGAATATGGTGTTGTGCGGCTAATTTCGCACAAAATTTCCGGGTAATATGTTTCCCGCTTTTACCCCGTACATTCGaataa                           >  2:412252/1‑140 (MQ=255)

                                                                                                            tAACAATCAGGTTTTCACCTTCGCCTTCGAGGGCGATGATGCGTTGTGCTTCATCGGCGGCTGTTAGGTGGAGTGAGACTTTTTGGCCACGAATTTGTCTGGCAAATTGATGACAGCTTTGACCACGTTCATTCGtataa                           >  2:339253/1‑140 (MQ=255)

                                                                                                                                     ttcgaGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCGGGTAATATGTTGCCAGCTTTCACCGCGTACATTCGAATAATGAAAACTTCCGGGGGCAGAGaaaa  <  1:293098/140‑1 (MQ=255)

CATGGTTAATGACTTCGTTATTCACCTCAACTGGTTGGGCGAGGGTAATAAAAAATCCACGAAAACGTCGCTCCTGTTCGCTCCAGCAATGTGGGATGGCGAGGCGATTAACAATCAGGTTTTCACCTTCGCCTTCGCGGGCGATGATGCGTTGTGCTTCATCGGCGGTAATATGGTGTTGTGCGACTAATTGCGCACAAATTTGCCGGGTAATATGTTGCCAGCTTTCACCGCGTACATTCGAATAATGAAAACTTCCGGGGGCAGAGAAAA  >  NC_000913/4088253‑4088525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A