Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F48 I1 R1 90 35.3 1199032 98.5% 1181046 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,266,928 C→A intergenic (+67/‑186) alr → / → tyrB alanine racemase 1/tyrosine aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,266,9280CA100.0% 101.4 / NA 30intergenic (+67/‑186)alr/tyrBalanine racemase 1/tyrosine aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/15);  total (15/15)

GAAAGTAAGCGCTTACGAACTTATTACGCGCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCCTAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCACTCTGTTGCTAATTGCCGTTCGCTCCTGAACATCCACTCGA  >  NC_000913/4266795‑4267050
                                                                                                                                     |                                                                                                                          
gAAAGTAAGCGCTTACGAACTTATTACGCGCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATaaaaa                                                                                                                      <  1:302429/140‑1 (MQ=255)
              aCGAACTTATTACGCGCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGAt                                                                                                        >  1:108873/1‑140 (MQ=255)
              aCGAACTTATTACGCGCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGAt                                                                                                        >  1:148243/1‑140 (MQ=255)
                         aCGCGCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATg                                                                                             <  1:216474/140‑1 (MQ=255)
                         aCGCGCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATg                                                                                             <  2:356778/140‑1 (MQ=255)
                          cgcgCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGc                                                                                            <  1:576528/140‑1 (MQ=255)
                          agcgcCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGc                                                                                            <  2:21079/139‑1 (MQ=255)
                              ccTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCtt                                                                                        >  2:143850/1‑140 (MQ=255)
                                            tCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGcc                                                                          <  1:229613/140‑1 (MQ=255)
                                                              aTTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGttt                                                        >  1:349854/1‑140 (MQ=255)
                                                                  aTCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCa                                                    >  2:495861/1‑140 (MQ=255)
                                                                   tCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCAt                                                   <  1:571185/140‑1 (MQ=255)
                                                                   acGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGTCTCTGGCGGATGTACGTTTGTCAt                                                   <  2:517047/139‑1 (MQ=255)
                                                                   acGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTAACCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCAt                                                   <  2:6886/139‑1 (MQ=255)
                                                                    cGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATg                                                  >  1:435859/1‑140 (MQ=255)
                                                                        cTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGtc                                              >  2:120981/1‑140 (MQ=255)
                                                                             atatTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCAct                                         <  1:141582/140‑1 (MQ=255)
                                                                              tatTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCa                                           <  1:361886/137‑1 (MQ=255)
                                                                              tatTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCa                                           >  2:361886/1‑137 (MQ=255)
                                                                                tttGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCActctt                                      >  2:273373/1‑139 (MQ=255)
                                                                                tttGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCActctt                                      >  2:469993/1‑139 (MQ=255)
                                                                                   gATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGGTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGCTTGTCATGAGTCTCACTCTGttt                                   >  1:251835/1‑139 (MQ=255)
                                                                                          tgtgCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTTTCATGAGTCTCACTCTGTTGCTAAttt                            >  2:34668/1‑139 (MQ=255)
                                                                                          tgtgCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCACTCTGTTGCTAATTg                            <  2:215168/140‑1 (MQ=255)
                                                                                               cGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCACTCTGTTGCTAATTGCCGtt                       <  2:108276/140‑1 (MQ=255)
                                                                                               cGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCACTCTGTTGCTAATTGCCGtt                       <  1:143850/140‑1 (MQ=255)
                                                                                                      ggcgTGAATGCCTTATCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCACTCTGTTGCTAATTGCCGTTCGCTCCt                <  1:495861/140‑1 (MQ=255)
                                                                                                                    aTCCGGCCATTAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCACTCTGTTGCTAATTGCCGTTCGCTCCTGAACATCCACTCGa  >  2:386588/1‑140 (MQ=255)
                                                                                                                    aTCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCACTCTGTTGCTAATTGCCGTTCGCTCCTGAACATCCACTCGa  >  2:429890/1‑140 (MQ=255)
                                                                                                                    aTCCGGCCAATAAAATCATAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCACTCTGTTGCTAATTGCCGTTCGCTCCTGAACATCCACTCGa  >  1:104032/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                          
GAAAGTAAGCGCTTACGAACTTATTACGCGCCTGACTTCAAGGGTCGCGATGAAATACGTGGATTAATCGTTCTGTAATATTTGATTGTCTGTGCCGGATGCGGCGTGAATGCCTTATCCGGCCAATAAAATCCTAAAAATTCAATAAGTTGATGTTCTTTCATGCTCTTATAAAGGTCGTGCCTCTGGCGGATGTACGTTTGTCATGAGTCTCACTCTGTTGCTAATTGCCGTTCGCTCCTGAACATCCACTCGA  >  NC_000913/4266795‑4267050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A