Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F48 I1 R1	62	93.4	3176452	99.2%	3151040	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,269,433	C→A	A7E (GCA→GAA)	aphA →	acid phosphatase/phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,269,433	0	C	A	99.0%	344.5 / ‑2.8	96	A7E (GCA→GAA)	aphA	acid phosphatase/phosphotransferase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (32/63); total (33/63)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

CACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGT  >  NC_000913/4269299‑4269565

cacaAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCa                                                                                                                                 <  1:814445/140‑1 (MQ=255)

cacaAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGCTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCa                                                                                                                                 <  2:617770/140‑1 (MQ=255)

  caAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGt                                                                                                                               >  1:1265169/1‑140 (MQ=255)

   aaaaaCCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTg                                                                                                                              <  1:1194614/140‑1 (MQ=255)

      aaCCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCg                                                                                                                           <  1:1563284/140‑1 (MQ=255)

      aaCCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCg                                                                                                                           <  1:570940/140‑1 (MQ=255)

       aCCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGCCTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGt                                                                                                                          <  1:1444418/140‑1 (MQ=255)

       aCCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGt                                                                                                                          <  1:41766/140‑1 (MQ=255)

             gATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCtt                                                                                                                    <  2:847435/140‑1 (MQ=255)

                 aTCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGG‑AAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGt                                                                                                               <  2:657567/140‑1 (MQ=255)

                  tccttcctTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGt                                                                                                               <  2:414900/140‑1 (MQ=255)

                  tccttcctTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGt                                                                                                               <  1:442474/140‑1 (MQ=255)

                  tccttcctTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGt                                                                                                               <  1:756861/140‑1 (MQ=255)

                  tccttcctTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGt                                                                                                               <  1:208736/140‑1 (MQ=255)

                    cttccttTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTc                                                                                                             <  2:1242356/140‑1 (MQ=255)

                     ttccttTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCg                                                                                                            <  2:290009/140‑1 (MQ=255)

                       ccttTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGct                                                                                                          >  1:862302/1‑140 (MQ=255)

                        cttTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGctc                                                                                                         >  2:781843/1‑140 (MQ=255)

                        cttTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTAACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAGTCAGTGCCGTTTGCTTATTGTTCGctc                                                                                                         >  1:530926/1‑140 (MQ=255)

                          ttCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTAACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAGTCAGTGCCGTTTGCTTATTGTTCGCTCTa                                                                                                       <  2:530926/140‑1 (MQ=255)

                           tCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTaa                                                                                                      <  1:758839/140‑1 (MQ=255)

                           tCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTaa                                                                                                      >  1:452844/1‑140 (MQ=255)

                           tCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTaa                                                                                                      >  1:1141398/1‑140 (MQ=255)

                            ccccGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTaaa                                                                                                     <  1:781843/140‑1 (MQ=255)

                             cccGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAAc                                                                                                    >  1:1212620/1‑140 (MQ=255)

                              ccGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACa                                                                                                   >  2:1567274/1‑140 (MQ=255)

                               cGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAg                                                                                                  >  1:339161/1‑140 (MQ=255)

                                 gCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGtt                                                                                                >  2:1269068/1‑140 (MQ=255)

                                    gCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATGCTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCg                                                                                             <  1:1186865/140‑1 (MQ=255)

                                    gCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCg                                                                                             <  1:1346207/140‑1 (MQ=255)

                                    gCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCg                                                                                             <  2:1212620/140‑1 (MQ=255)

                                       ggCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTg                                                                                          <  1:393363/140‑1 (MQ=255)

                                       ggCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTg                                                                                          <  2:1036443/140‑1 (MQ=255)

                                       ggCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTg                                                                                          <  2:272916/140‑1 (MQ=255)

                                       ggCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTg                                                                                          >  2:90485/1‑140 (MQ=255)

                                       ggCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTg                                                                                          <  1:567417/140‑1 (MQ=255)

                                       ggCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTg                                                                                          <  2:1295779/140‑1 (MQ=255)

                                            tATGGTCAGATGGTTTTTGGAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGTTTATTATTCGCTCTAGACAGTTCCGCTGTTGcc                                                                                     <  2:1040338/140‑1 (MQ=255)

                                            tATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGcc                                                                                     <  2:339161/140‑1 (MQ=255)

                                               ggTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTg                                                                                  <  1:1052627/140‑1 (MQ=255)

                                                  cAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGcc                                                                               <  2:1265169/140‑1 (MQ=255)

                                                    gATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTc                                                                             <  1:1567274/140‑1 (MQ=255)

                                                        gTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCAtct                                                                         >  2:1050704/1‑140 (MQ=255)

                                                          ttttGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTcc                                                                       >  1:1108226/1‑140 (MQ=255)

                                                          ttttGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTcc                                                                       >  1:1566813/1‑140 (MQ=255)

                                                            ttGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCtt                                                                     <  1:359892/140‑1 (MQ=255)

                                                            ttGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCtt                                                                     <  1:886571/140‑1 (MQ=255)

                                                             tGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTc                                                                    >  2:1345593/1‑140 (MQ=255)

                                                             tGCAACAAATCTCACAATAAAAAGGTTTAACATACGGGCTGTATAGGGAAAAATATCCGCAAGATCACACAGGAAATCAGTTCCGTTTGCTTATTGTTCGCTCTATACAGTTCCGCTGTTGCACTGGCCTCATCTCCTTc                                                                    <  2:274402/140‑1 (MQ=255)

                                                              gCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCa                                                                   <  1:665562/140‑1 (MQ=255)

                                                              gCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCa                                                                   <  2:862302/140‑1 (MQ=255)

                                                                aacaaATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCAcc                                                                 <  2:173452/140‑1 (MQ=255)

                                                                   aaaTCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCt                                                              <  2:200965/140‑1 (MQ=255)

                                                                   aaaTCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCt                                                              >  1:537744/1‑140 (MQ=255)

                                                                   aaaTCTCACAATAAAAAGTTTAAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTACTGTACGATCTAAGCAGTTGCGATGTTGCCCTGGCCTCATCTCTTTCACCGCt                                                              <  2:1385009/140‑1 (MQ=255)

                                                                    aaTCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCtt                                                             <  2:70163/140‑1 (MQ=255)

                                                                    aaTCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCtt                                                             <  1:90485/140‑1 (MQ=255)

                                                                     aTCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTa                                                            <  1:1269068/140‑1 (MQ=255)

                                                                         cacaATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAAccc                                                        <  2:1108226/140‑1 (MQ=255)

                                                                         cacaATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAAccc                                                        <  2:1566813/140‑1 (MQ=255)

                                                                            aaTAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTgg                                                     <  2:353801/140‑1 (MQ=255)

                                                                              tAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGa                                                   >  2:1168417/1‑140 (MQ=255)

                                                                                  aaGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTaa                                               <  2:537744/140‑1 (MQ=255)

                                                                                   aGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAAc                                              <  1:1279438/140‑1 (MQ=255)

                                                                                    gTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACg                                             <  1:1097618/140‑1 (MQ=255)

                                                                                      ttCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGtt                                           >  2:999484/1‑140 (MQ=255)

                                                                                      ttCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGtt                                           >  1:195382/1‑140 (MQ=255)

                                                                                      ttCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGtt                                           >  2:508064/1‑140 (MQ=255)

                                                                                        cAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGc                                         >  2:1321200/1‑140 (MQ=255)

                                                                                           cATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAg                                      <  1:403631/140‑1 (MQ=255)

                                                                                           aaTACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAg                                      <  2:144193/139‑1 (MQ=255)

                                                                                              aCTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCt                                   >  2:376989/1‑140 (MQ=255)

                                                                                                tGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTg                                 <  1:863960/140‑1 (MQ=255)

                                                                                                tGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTg                                 <  1:303724/140‑1 (MQ=255)

                                                                                                 gACTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGc                                <  2:1160229/140‑1 (MQ=255)

                                                                                                  aCTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCt                               >  1:1534323/1‑140 (MQ=255)

                                                                                                  aCTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCt                               >  2:1339531/1‑140 (MQ=255)

                                                                                                   cTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTg                              >  2:294422/1‑140 (MQ=255)

                                                                                                   cTATTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTg                              <  1:965781/140‑1 (MQ=255)

                                                                                                     aTTTAGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGaa                            >  1:494845/1‑140 (MQ=255)

                                                                                                         aGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAgg                        <  2:700373/140‑1 (MQ=255)

                                                                                                         aGGGAAAAATATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAgg                        <  2:662613/140‑1 (MQ=255)

                                                                                                             aaaaaTATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCAcc                    <  2:1534323/140‑1 (MQ=255)

                                                                                                              aaaaTATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCt                               <  2:804366/128‑1 (MQ=255)

                                                                                                              aaaaTATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCt                               >  1:804366/1‑128 (MQ=255)

                                                                                                              aaaaTATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCAccc                   >  2:530207/1‑140 (MQ=255)

                                                                                                              aaaaTATGCGCAAGATCACACAGGAAATCAGTGCCATTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCAccc                   >  2:799420/1‑140 (MQ=255)

                                                                                                                aaTATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCcat                 >  2:699485/1‑140 (MQ=255)

                                                                                                                aaTATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCcat                 <  1:508064/140‑1 (MQ=255)

                                                                                                                aaTATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCcat                 >  1:691373/1‑140 (MQ=255)

                                                                                                                aaTATGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCcat                 <  1:1302768/140‑1 (MQ=255)

                                                                                                                   atGCGCAAGATCACACAGGAAATGAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCcattca              <  2:944449/140‑1 (MQ=255)

                                                                                                                   atGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACAcattca              <  2:1262264/140‑1 (MQ=255)

                                                                                                                    tGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCcattcat             <  1:238637/140‑1 (MQ=255)

                                                                                                                    tGCGCAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCcattcat             <  1:1332943/140‑1 (MQ=255)

                                                                                                                     gcgcAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCcattcatt            >  2:364945/1‑140 (MQ=255)

                                                                                                                      cgcAAGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTg           <  2:906285/140‑1 (MQ=255)

                                                                                                                          aGATCACACAGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGtt       >  1:525859/1‑140 (MQ=255)

                                                                                                                               acacaGGAAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGt  <  2:510213/140‑1 (MQ=255)

CACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGT  >  NC_000913/4269299‑4269565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A