Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F48 I1 R1 62 147.6 5031800 99.1% 4986513 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,223,261 G→T A123E (GCG→GAG)  iclR ← DNA‑binding transcriptional repressor IclR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,2610GT98.8% 285.0 / ‑3.1 81A123E (GCG→GAG) iclRDNA‑binding transcriptional repressor IclR
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (30/50);  total (31/50)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

AAAGGCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGATTGCC  >  NC_000913/4223134‑4223383
                                                                                                                               |                                                                                                                          
aagggttttCCCCGCACCGGAAGCGTGCATCGGAAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCg                                                                                                                <  1:933382/134‑1 (MQ=255)
aaaGGCTTTCCCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCg                                                                                                                <  1:220029/140‑1 (MQ=255)
    gCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTc                                                                                                            >  1:1067361/1‑140 (MQ=255)
    gCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTc                                                                                                            >  2:852286/1‑140 (MQ=255)
     ttttCCCCGCCCCGGAAGCGTGCATCGGAATTTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCg                                                                                                           <  2:1619231/139‑1 (MQ=255)
     ttttCCCCGCACGGGAAGCGTGCATCGGCATTTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCg                                                                                                           <  2:1519126/139‑1 (MQ=255)
     cttcccccGCCCCGGAAGCGTGAATCGGAAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCg                                                                                                           <  2:2324527/135‑1 (MQ=255)
     cTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTct                                                                                                           >  2:72152/1‑139 (MQ=255)
      tttACCCGCCCCGGAAGCGTGCATGGGAATTTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGc                                                                                                          <  2:1320681/140‑1 (MQ=255)
         aCCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAg                                                                                                       >  2:2026162/1‑140 (MQ=255)
         aCCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAg                                                                                                       >  1:556/1‑140 (MQ=255)
          cccgcccgGGAGGCGTGCATGGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGa                                                                                                      <  1:1745129/140‑1 (MQ=255)
          cccGCACGGGAGGCGTGCAGCGGAAATTTCCCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGa                                                                                                      <  2:484575/140‑1 (MQ=255)
          cccGCACGGGAAGCGTGCTTCGGAAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGa                                                                                                      <  2:1511786/140‑1 (MQ=255)
          cccGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGa                                                                                                      >  2:1349017/1‑140 (MQ=255)
             ccACCGAAAGCGGGCATCGCCAATTAACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGActc                                                                                                   <  1:2388563/139‑1 (MQ=255)
               aCCGGAAGCGTGCATGGGAATTTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCtt                                                                                                 <  2:82310/140‑1 (MQ=255)
                       ggTGCATCGACATTTTGCCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGa                                                                                         <  1:946725/139‑1 (MQ=255)
                       cGTGCGTCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGa                                                                                         <  1:1369741/140‑1 (MQ=255)
                       cGTGCATCGGCATTTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGa                                                                                         <  1:2352747/140‑1 (MQ=255)
                       cGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGa                                                                                         <  2:556/140‑1 (MQ=255)
                       cGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGa                                                                                         <  1:651874/140‑1 (MQ=255)
                       cGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGa                                                                                         <  1:400272/140‑1 (MQ=255)
                         tGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGAtt                                                                                       >  2:1403562/1‑140 (MQ=255)
                         tGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGAtt                                                                                       >  1:649070/1‑140 (MQ=255)
                         tGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGAtt                                                                                       >  1:1391272/1‑140 (MQ=255)
                         tGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTAGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGAtt                                                                                       >  2:602600/1‑140 (MQ=255)
                           cATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgc                                                                                     <  1:1584400/140‑1 (MQ=255)
                           cATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgc                                                                                     <  2:488821/140‑1 (MQ=255)
                           cATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgc                                                                                     <  2:1212395/140‑1 (MQ=255)
                           cATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgc                                                                                     <  1:1298338/140‑1 (MQ=255)
                           cATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgc                                                                                     <  2:1104634/140‑1 (MQ=255)
                           aatgggaaaTTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgc                                                                                     <  2:335881/133‑1 (MQ=255)
                            aTCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTTGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcg                                                                                    <  2:842229/140‑1 (MQ=255)
                            aTCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcg                                                                                    <  1:2026162/140‑1 (MQ=255)
                            aTCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcg                                                                                    >  2:660297/1‑140 (MQ=255)
                             acGGAAATTAACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTgcgc                                                                                   <  1:933190/139‑1 (MQ=255)
                                gCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAgg                                                                                <  2:1067361/140‑1 (MQ=255)
                                gCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAgg                                                                                >  1:2445473/1‑140 (MQ=255)
                                          gcctATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATAGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGaa                                                                      <  1:2183426/140‑1 (MQ=255)
                                          gccgATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGaa                                                                      <  2:2445473/140‑1 (MQ=255)
                                              aTAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAAt                                                                  >  1:703191/1‑140 (MQ=255)
                                                  gcgcGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCt                                                              <  2:1932649/140‑1 (MQ=255)
                                                           ttCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTa                                                     <  1:2314923/140‑1 (MQ=255)
                                                            tCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTAc                                                    >  1:1145748/1‑140 (MQ=255)
                                                            tCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTAc                                                    >  2:1322064/1‑140 (MQ=255)
                                                             agCATCAGATGCGTACACTGTAACTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACg                                                   <  2:880794/139‑1 (MQ=255)
                                                                aTCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGct                                                <  1:712050/140‑1 (MQ=255)
                                                                  cAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGctct                                              >  2:51097/1‑140 (MQ=255)
                                                                  cAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGctct                                              >  2:464718/1‑140 (MQ=255)
                                                                      tGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGgag                                          <  1:1848766/140‑1 (MQ=255)
                                                                               tGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAgctgct                                 >  1:532056/1‑140 (MQ=255)
                                                                                gTACCTGGTCGATAATAATCGCTTCGTGATAGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGctgctg                                <  2:703191/140‑1 (MQ=255)
                                                                                 tACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCtgctgc                               >  1:479794/1‑140 (MQ=255)
                                                                                 tACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCtgctgc                               >  2:811537/1‑140 (MQ=255)
                                                                                 tACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAAAAAATTACGGCTCTGGAGAAAGCtgctgc                               >  1:1484947/1‑140 (MQ=255)
                                                                                 tACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCtgctgc                               >  1:780127/1‑140 (MQ=255)
                                                                                  aCCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCGCCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGcc                              <  2:1653483/140‑1 (MQ=255)
                                                                                  aCCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCTGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGcc                              <  1:602600/140‑1 (MQ=255)
                                                                                  aCCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGcc                              >  2:94966/1‑140 (MQ=255)
                                                                                  aCCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGcc                              <  1:1349017/140‑1 (MQ=255)
                                                                                  aCCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGcc                              >  1:1168924/1‑140 (MQ=255)
                                                                                  aCCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGcc                              <  2:1393172/140‑1 (MQ=255)
                                                                                  aCCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGcc                              <  1:186943/140‑1 (MQ=255)
                                                                                  aCCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGcc                              <  1:2459566/140‑1 (MQ=255)
                                                                                   ccTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCg                             >  1:958018/1‑140 (MQ=255)
                                                                                    cTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGa                            <  1:852286/140‑1 (MQ=255)
                                                                                    cTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGa                            <  1:185231/140‑1 (MQ=255)
                                                                                    cTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGa                            <  2:894224/140‑1 (MQ=255)
                                                                                    cTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGa                            <  1:811537/140‑1 (MQ=255)
                                                                                    cTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGa                            <  2:1391272/140‑1 (MQ=255)
                                                                                      ggTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGAcc                          >  1:920685/1‑140 (MQ=255)
                                                                                       gTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCa                         >  1:1907151/1‑140 (MQ=255)
                                                                                       gTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCa                         >  2:1278667/1‑140 (MQ=255)
                                                                                                  tCGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCAtgt              >  2:1888456/1‑140 (MQ=255)
                                                                                                   cGCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCAtgtg             <  2:1145748/140‑1 (MQ=255)
                                                                                                    gCTTCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCGTGTgc            <  2:920685/140‑1 (MQ=255)
                                                                                                      ttCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTgcgc          >  2:1757877/1‑140 (MQ=255)
                                                                                                      ttCGTGATCGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTgcgc          >  1:1700388/1‑140 (MQ=255)
                                                                                                       tCGTGATCGCTTTGATCAAGTACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGcc         <  2:1700388/140‑1 (MQ=255)
                                                                                                              cGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGATTGcc  <  1:518380/140‑1 (MQ=255)
                                                                                                              cGCTTTGATCAAGCACCTCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGATTGcc  <  1:660297/140‑1 (MQ=255)
                                                                                                                               |                                                                                                                          
AAAGGCTTTACCCGCACCGGAAGCGTGCATCGGCAATTTACCGCCGATAGGCGCGGACATTCGCATCAGATGCGTACACTGTACCTGGTCGATAATAATCGCTTCGTGATCGCTTTGATCAAGCACCGCCATATTGACCGTTTCGCCAGACTCTTCCATTAGATTGCGCAGGATAGGGTGAACAATCGCTAACAAATTACGGCTCTGGAGAAAGCTGCTGCCGACCATAAAGGCATGTGCGCCGATTGCC  >  NC_000913/4223134‑4223383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A