Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F48 I1 R1	52	61.9	2103308	98.8%	2078068	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,961,072	C→A	A453S (GCG→TCG)	recC ←	exodeoxyribonuclease V subunit RecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,961,072	0	C	A	100.0%	184.0 / NA	54	A453S (GCG→TCG)	recC	exodeoxyribonuclease V subunit RecC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (28/26); total (28/26)

TTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCACGAT  >  NC_000913/2960936‑2961204

ttGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTc                                                                                                                                   >  2:72580/1‑140 (MQ=255)

 tGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTcc                                                                                                                                  <  1:232025/140‑1 (MQ=255)

         tGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCgg                                                                                                                          >  1:639758/1‑140 (MQ=255)

                     cGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCcg                                                                                                              >  1:35034/1‑140 (MQ=255)

                     cGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCcg                                                                                                              >  1:781923/1‑140 (MQ=255)

                     cGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCcg                                                                                                              >  1:741280/1‑140 (MQ=255)

                         gCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCa                                                                                                          >  1:55363/1‑140 (MQ=255)

                             ttcttcGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCacgac                                                                                                      >  1:272041/1‑140 (MQ=255)

                             ttcttcGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCacgac                                                                                                      >  1:422479/1‑140 (MQ=255)

                               cttcGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACgg                                                                                                    >  2:548131/1‑140 (MQ=255)

                               cttcGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACgg                                                                                                    >  2:16747/1‑140 (MQ=255)

                                    gTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGa                                                                                               >  2:554243/1‑140 (MQ=255)

                                    gTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGa                                                                                               >  1:792503/1‑140 (MQ=255)

                                      gATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGaaa                                                                                             <  2:53443/140‑1 (MQ=255)

                                           cAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCg                                                                                        <  1:587223/140‑1 (MQ=255)

                                            aaaCCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGt                                                                                       >  2:975019/1‑140 (MQ=255)

                                             aaCCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGAAGGTCGGAAATGGCGTa                                                                                      <  2:55363/140‑1 (MQ=255)

                                              aCCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTaa                                                                                     <  2:931368/140‑1 (MQ=255)

                                               ccGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAag                                                                                    <  1:554243/140‑1 (MQ=255)

                                               ccGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAag                                                                                    <  1:975019/140‑1 (MQ=255)

                                               ccGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCCCGCACGACGGTCGGAAATGGCGTAag                                                                                    <  2:741280/140‑1 (MQ=255)

                                                 gcgcCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAaggt                                                                                  <  1:1036927/140‑1 (MQ=255)

                                                 gcgcCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAaggt                                                                                  <  2:781923/140‑1 (MQ=255)

                                                   gccgccAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAaggtag                                                                                >  1:68887/1‑140 (MQ=255)

                                                      gccAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAggtaggta                                                                             <  1:644917/140‑1 (MQ=255)

                                                       ccAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTaa                                                                            <  1:72580/140‑1 (MQ=255)

                                                       ccAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTaa                                                                            <  2:272041/140‑1 (MQ=255)

                                                          gCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGa                                                                         <  2:68887/140‑1 (MQ=255)

                                                                  acaTCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAgg                                                                 >  1:437860/1‑140 (MQ=255)

                                                                    aTCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCGGATAAACGATTCCAGTACCGGATGTGACTGCGGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTg                                                               <  2:35034/140‑1 (MQ=255)

                                                                     tCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTgc                                                              >  1:19925/1‑140 (MQ=255)

                                                                     tCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTgc                                                              >  1:484647/1‑140 (MQ=255)

                                                                      ccAGCAACGTCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTgcg                                                             <  1:930997/140‑1 (MQ=255)

                                                                      ccAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTgcg                                                             <  2:333995/140‑1 (MQ=255)

                                                                      ccAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTgcg                                                             <  1:354689/140‑1 (MQ=255)

                                                                      ccAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTgcg                                                             <  2:380437/140‑1 (MQ=255)

                                                                      ccAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTgcg                                                             <  2:639758/140‑1 (MQ=255)

                                                                          cAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTAc                                                         <  1:548131/140‑1 (MQ=255)

                                                                          aaaCGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTAc                                                         <  2:422479/139‑1 (MQ=255)

                                                                                cAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAaca                                                   <  2:484647/140‑1 (MQ=255)

                                                                                   cacaTCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAg                                                >  2:54994/1‑140 (MQ=255)

                                                                                        ccTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGa                                           >  2:203895/1‑140 (MQ=255)

                                                                                             gACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATaaa                                      >  1:917994/1‑140 (MQ=255)

                                                                                             aacacAAAACGACTGTCAAGCAGCGATAACAGGCTGATAAACGATTCAAGTATCGGATGTGACTGCCGCGCGCGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATaaa                                      <  2:626523/139‑1 (MQ=255)

                                                                                              acacAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCTGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAc                                     >  1:242061/1‑140 (MQ=255)

                                                                                              acacAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAAc                                     <  2:437860/140‑1 (MQ=255)

                                                                                                 caAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGa                                  <  1:16747/140‑1 (MQ=255)

                                                                                                              aGGCAGTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGa                     >  1:397919/1‑140 (MQ=255)

                                                                                                                   gTGATAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCa                >  2:566665/1‑140 (MQ=255)

                                                                                                                      aTAACAGGCAGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGcc             >  2:679370/1‑140 (MQ=255)

                                                                                                                       tAACAGGCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGcca            <  1:1040254/140‑1 (MQ=255)

                                                                                                                            ggCTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATc       >  2:252929/1‑140 (MQ=255)

                                                                                                                              cTGATAAACGATTCTAGATCCGGATGTGACTGCCGCGTACGTCGGTCGGAAATGGCGTAAGGAAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCAc     >  2:660525/1‑140 (MQ=255)

                                                                                                                              cTGATAAACGATTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCAc     >  2:673826/1‑140 (MQ=255)

                                                                                                                                 aTAAACGATTCCAGTACCGGATGCGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCACgat  >  2:685087/1‑140 (MQ=255)

TTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTAAGGTAGGTAACGATCCGCAGGTGCGCTACCAAACACAGCCTGAATAAACGGACTGTAGCTGTCGATATCAGCCACCATCACGAT  >  NC_000913/2960936‑2961204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A