Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F48 I1 R1	52	61.9	2103308	98.8%	2078068	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,202,877	G→T	W329C (TGG→TGT)	cca →	fused tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase and phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,202,877	0	G	T	100.0%	169.6 / NA	48	W329C (TGG→TGT)	cca	fused tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase and phosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (31/17); total (31/17)

ATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGGCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTGGCGCAGTCAGTGCC  >  NC_000913/3202742‑3203011

aTGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTa                                                                                                                                    >  2:935252/1‑140 (MQ=255)

         tCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGc                                                                                                                           <  1:2753/140‑1 (MQ=255)

           tGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAACgt                                                                                                                         >  1:956208/1‑140 (MQ=255)

             cgcgTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCgtgt                                                                                                                       >  2:1038994/1‑140 (MQ=255)

                       aTGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTg                                                                                                             >  2:708385/1‑140 (MQ=255)

                                  gATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAg                                                                                                  <  1:330683/140‑1 (MQ=255)

                                       aGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAgg                                                                                             <  1:782731/140‑1 (MQ=255)

                                               tggtggCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAACGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTg                                                                                     <  2:956208/140‑1 (MQ=255)

                                                 gtggCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTgcg                                                                                   >  2:139423/1‑140 (MQ=255)

                                                    gCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCgg                                                                                >  2:39088/1‑140 (MQ=255)

                                                     cTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGc                                                                               <  1:1038994/140‑1 (MQ=255)

                                                        aGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGa                                                                            >  1:1029636/1‑140 (MQ=255)

                                                          tttCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAAc                                                                          <  1:935252/140‑1 (MQ=255)

                                                           ttCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAAcc                                                                         <  1:774758/140‑1 (MQ=255)

                                                           ttCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAAcc                                                                         <  2:1009411/140‑1 (MQ=255)

                                                            tCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCg                                                                        >  2:833364/1‑140 (MQ=255)

                                                                    tcATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGaa                                                                >  1:69256/1‑140 (MQ=255)

                                                                       tCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCa                                                             >  1:852761/1‑140 (MQ=255)

                                                                         cgcacCTGCCAAAAGCTGAACCAAAAAACCATCGGCAAATTATTTGATTCCATGGAGGCGTGTGGTAAGCCGCGGCGGGTGGAGCAACGGTCCGTGGCCAGCGAGGCTGACGTGCGCGGCAGAAGAAGTTTTGaaaaagt                                                           <  2:1008347/138‑6 (MQ=255)

                                                                            acCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGa                                                        >  2:507445/1‑140 (MQ=255)

                                                                             ccTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGTGCGGCAGAACCGGTTTTGAATCAGCGGAc                                                       <  1:490805/140‑1 (MQ=255)

                                                                              cTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACt                                                      >  1:800095/1‑140 (MQ=255)

                                                                                tCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTAc                                                    >  2:223294/1‑140 (MQ=255)

                                                                                tCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTAc                                                    >  2:539087/1‑140 (MQ=255)

                                                                                tCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTAc                                                    >  2:342443/1‑140 (MQ=255)

                                                                                tCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTAc                                                    >  1:823043/1‑140 (MQ=255)

                                                                                tCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTT‑‑‑‑TCAGCGGACTACCCGc                                                >  2:943528/1‑140 (MQ=255)

                                                                                 cccAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTAcc                                                   >  1:57494/1‑140 (MQ=255)

                                                                                     aTGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCa                                               <  2:368782/140‑1 (MQ=255)

                                                                                     aTGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCa                                               <  2:642785/140‑1 (MQ=255)

                                                                                          gAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGcc                                          <  2:57494/140‑1 (MQ=255)

                                                                                           aaCCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCg                                         >  1:673624/1‑140 (MQ=255)

                                                                                           aaCCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCg                                         >  1:59997/1‑140 (MQ=255)

                                                                                           aaCCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCg                                         >  2:524781/1‑140 (MQ=255)

                                                                                               cAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTgg                                     <  2:69256/140‑1 (MQ=255)

                                                                                                aaaaaCCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGt                                    >  2:953515/1‑140 (MQ=255)

                                                                                                 aaaaCCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGtt                                   >  1:950510/1‑140 (MQ=255)

                                                                                                    aCCATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTgcg                                <  1:223294/140‑1 (MQ=255)

                                                                                                     ccATCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTgcgc                               <  1:953515/140‑1 (MQ=255)

                                                                                                       aTCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGa                             <  1:539087/140‑1 (MQ=255)

                                                                                                       aTCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGa                             <  1:703528/140‑1 (MQ=255)

                                                                                                        tCGTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGaa                            >  2:560588/1‑140 (MQ=255)

                                                                                                          gTCAAATTATTTGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGc                          >  1:45432/1‑140 (MQ=255)

                                                                                                                    ttGATTCCGTCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTg                >  2:14030/1‑140 (MQ=255)

                                                                                                                     tGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTgg               >  1:850211/1‑140 (MQ=255)

                                                                                                                     tGATTCCATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTgg               >  1:428385/1‑140 (MQ=255)

                                                                                                                           cATCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTGGCGcagt         <  2:852761/140‑1 (MQ=255)

                                                                                                                             tCGATGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTGGCGcagtca       >  2:943490/1‑140 (MQ=255)

                                                                                                                                aTGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTGGCGCAGTCAGTg    <  1:507445/140‑1 (MQ=255)

                                                                                                                                 tGCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTGGCGCAGTCAGTGc   >  2:499147/1‑140 (MQ=255)

                                                                                                                                  gCCTGTCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTAGTTGCGCGAAGCCTGGGAAGTGGCGCAGTCAGTGcc  >  1:19898/1‑140 (MQ=255)

ATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCCAGACTGGTGGCTGAGTTTCACGATCTCATCCACACCTTCCCAATGCTGAACCCAAAAACCATCGTCAAATTATTTGATTCCATCGATGCCTGGCGTAAACCGCAGCGTGTCGAGCAACTGGCGCTGACCAGCGAGGCTGACGTGCGCGGCAGAACCGGTTTTGAATCAGCGGACTACCCGCAAGGCCGCTGGTTGCGCGAAGCCTGGGAAGTGGCGCAGTCAGTGCC  >  NC_000913/3202742‑3203011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A