Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F48 I1 R1 55 131.7 4456318 99.2% 4420667 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,203,636 G→T L166M (CTG→ATG)  bacA ← undecaprenyl pyrophosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,203,6360GT97.3% 387.1 / 3.7 112L166M (CTG→ATG) bacAundecaprenyl pyrophosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (58/51);  total (59/53)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 113 total aligned reads displayed.

TCGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCAGACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCATACAT  >  NC_000913/3203500‑3203765
                                                                                                                                        |                                                                                                                                 
tCGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATaca                                                                                                                                <  2:898391/140‑1 (MQ=255)
ccGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATaca                                                                                                                                <  1:1497920/139‑1 (MQ=255)
 cGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATacac                                                                                                                               >  2:452481/1‑140 (MQ=255)
 cGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATacac                                                                                                                               >  1:610877/1‑140 (MQ=255)
 cGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATacac                                                                                                                               <  1:1155308/140‑1 (MQ=255)
 cGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATacac                                                                                                                               >  1:1775975/1‑140 (MQ=255)
 cGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATacac                                                                                                                               >  1:1297868/1‑140 (MQ=255)
    gcgcCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGCAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTgg                                                                                                                            >  2:453670/1‑140 (MQ=255)
       ccGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGaaa                                                                                                                         >  2:110299/1‑140 (MQ=255)
         gTTGTGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACa                                                                                                                       >  1:1333178/1‑140 (MQ=255)
          ttGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAg                                                                                                                      >  2:321868/1‑140 (MQ=255)
          ttGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAg                                                                                                                      >  1:1856600/1‑140 (MQ=255)
          ttGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAg                                                                                                                      >  1:1717714/1‑140 (MQ=255)
          ttGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAg                                                                                                                      >  1:932699/1‑140 (MQ=255)
             cgcCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCa                                                                                                                   >  2:1063265/1‑140 (MQ=255)
                  atcatcatcGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCAt                                                                                                              >  1:446770/1‑140 (MQ=255)
                    catcatcGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATaa                                                                                                            >  2:760623/1‑140 (MQ=255)
                     atcatcGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATaaa                                                                                                           <  1:1996714/140‑1 (MQ=255)
                     atcatcGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATaaa                                                                                                           <  1:760623/140‑1 (MQ=255)
                     atcatcGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATaaa                                                                                                           <  1:2050953/140‑1 (MQ=255)
                         tcGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGcc                                                                                                       <  2:1856600/140‑1 (MQ=255)
                          cGGCTCCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCAGCAGCATCCCACCGTAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCAGACACTGGAAACAGCCAATCATAAATGCCt                                                                                                      <  1:2137641/140‑1 (MQ=255)
                          cGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCt                                                                                                      >  1:2141575/1‑140 (MQ=255)
                            gCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGa                                                                                                    >  1:113299/1‑140 (MQ=255)
                             cACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGAc                                                                                                   >  2:1266307/1‑140 (MQ=255)
                              aCCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACg                                                                                                  >  2:1440288/1‑140 (MQ=255)
                              aCCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACg                                                                                                  >  2:1518379/1‑140 (MQ=255)
                              aCCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACg                                                                                                  >  2:1626725/1‑140 (MQ=255)
                              aCCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACg                                                                                                  >  2:212569/1‑140 (MQ=255)
                              aCCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACg                                                                                                  <  2:283609/140‑1 (MQ=255)
                              aCCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACg                                                                                                  <  2:446770/140‑1 (MQ=255)
                              aCCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACg                                                                                                  <  1:1063265/140‑1 (MQ=255)
                               ccGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGa                                                                                                 >  1:151313/1‑140 (MQ=255)
                                cGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGAt                                                                                                <  1:2129172/140‑1 (MQ=255)
                                cGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGAt                                                                                                >  1:2211693/1‑140 (MQ=255)
                                  ccAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGa                                                                                                    <  1:1363450/134‑1 (MQ=255)
                                  ccAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGa                                                                                                    >  2:1363450/1‑134 (MQ=255)
                                   aagcagGAACGAAAACTCGGCAGCAGCGTAACGGCTCACCCACATCAGCATCCCACCTAAAAGGGTCGCAACGAAACGGGAGAAACCCAGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAgg                                                                                             <  2:1498322/139‑1 (MQ=255)
                                    agcagGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGt                                                                                            <  2:2211693/140‑1 (MQ=255)
                                    agcagGAACGAAAACTCGGAAGCAGCATAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGt                                                                                            <  1:224990/140‑1 (MQ=255)
                                        ggAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCata                                                                                        <  2:834937/140‑1 (MQ=255)
                                         gAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCatat                                                                                       <  1:110299/140‑1 (MQ=255)
                                          aaCGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATatc                                                                                      >  2:1438755/1‑140 (MQ=255)
                                                             cGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCAcgc                                                                   >  1:515843/1‑140 (MQ=255)
                                                               tAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCgg                                                                 <  1:450716/140‑1 (MQ=255)
                                                               aaaCTGCTCACCCCCATCAGCATCCCACCTGAAAAGTGCGCCCCGGAACGGGAGAAACCCGGCCACAGCGACATACACTGGAAACAGCCAAACATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCgg                                                                 <  2:608107/139‑1 (MQ=255)
                                                                aaCGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGc                                                                <  1:1438755/140‑1 (MQ=255)
                                                                     cTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCAAATCATCAAGACCCGGCGCACGCGGCTCttt                                                           <  2:113299/140‑1 (MQ=255)
                                                                       cACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCg                                                         >  1:1564204/1‑140 (MQ=255)
                                                                       cACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCg                                                         >  2:1177617/1‑140 (MQ=255)
                                                                       cACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCAGACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCg                                                         >  2:2118068/1‑140 (MQ=255)
                                                                          ccccATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCt                                                      >  1:1028802/1‑140 (MQ=255)
                                                                               tCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAgg                                                 >  2:1862674/1‑140 (MQ=255)
                                                                               tCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAga                                                 >  2:1888367/1‑139 (MQ=255)
                                                                               tCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCAGACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAgg                                                 <  1:2118068/140‑1 (MQ=255)
                                                                                 aGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCa                                               <  1:1266307/140‑1 (MQ=255)
                                                                                    aTCCCACCTGAAATGGTCGCCTCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTc                                            >  1:332574/1‑140 (MQ=255)
                                                                                    aTCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTc                                            >  1:1517300/1‑140 (MQ=255)
                                                                                    aTCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTc                                            >  2:1532929/1‑140 (MQ=255)
                                                                                      cccACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTcgg                                          <  1:321868/140‑1 (MQ=255)
                                                                                         aCCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTcggcgg                                       <  2:1297868/140‑1 (MQ=255)
                                                                                          ccTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCggcggc                                      >  1:1309793/1‑140 (MQ=255)
                                                                                          ccTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCggcggc                                      >  2:2162910/1‑140 (MQ=255)
                                                                                                  ggTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGca                              >  1:1363549/1‑140 (MQ=255)
                                                                                                   gTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGcaa                             >  2:1493812/1‑140 (MQ=255)
                                                                                                   gTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGcaa                             >  1:2208002/1‑140 (MQ=255)
                                                                                                   gTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGcaa                             >  2:810976/1‑140 (MQ=255)
                                                                                                     cGCCTCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGcaaca                           <  2:332574/140‑1 (MQ=255)
                                                                                                     cGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGcaaca                           >  2:1231077/1‑140 (MQ=255)
                                                                                                     cGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGcaaca                           <  2:1028802/140‑1 (MQ=255)
                                                                                                     cGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGcaaca                           >  1:573331/1‑140 (MQ=255)
                                                                                                     cGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGcaaca                           >  2:615052/1‑140 (MQ=255)
                                                                                                     cGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGcaaca                           >  2:1497395/1‑140 (MQ=255)
                                                                                                        cccGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCTTTCGGCGGCAATCAGCAACAAAc                        <  1:1689334/140‑1 (MQ=255)
                                                                                                        cccGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAAc                        >  1:1176912/1‑140 (MQ=255)
                                                                                                        cccGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAAc                        <  2:1969735/140‑1 (MQ=255)
                                                                                                         ccGGAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGc                               <  2:1707320/132‑1 (MQ=255)
                                                                                                            gAACGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGcc                    <  1:975396/140‑1 (MQ=255)
                                                                                                               cGGGAGAAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAAc                 <  1:1130908/140‑1 (MQ=255)
                                                                                                                  gagaAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGAc              <  1:1532929/140‑1 (MQ=255)
                                                                                                                  gagaAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGAc              >  1:1960615/1‑140 (MQ=255)
                                                                                                                  gagaAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGAc              <  2:491790/140‑1 (MQ=255)
                                                                                                                  gagaAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGAc              <  2:1363549/140‑1 (MQ=255)
                                                                                                                  gagaAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGAc              <  2:2208002/140‑1 (MQ=255)
                                                                                                                   agaAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGAcc             <  2:2141575/140‑1 (MQ=255)
                                                                                                                   agaAACCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGAcc             <  1:139500/140‑1 (MQ=255)
                                                                                                                     aaaCCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAg           <  2:1960615/140‑1 (MQ=255)
                                                                                                                      aaCCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAgc          >  2:2211050/1‑140 (MQ=255)
                                                                                                                       aCCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAgc          <  2:433581/139‑1 (MQ=255)
                                                                                                                       aCCCGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAgc          >  1:433581/1‑139 (MQ=255)
                                                                                                                        cccGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAgcgc        <  2:1333178/140‑1 (MQ=255)
                                                                                                                        cccGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAgcgc        <  1:1827474/140‑1 (MQ=255)
                                                                                                                         ccGGCCACAGCGCCATACACTGAAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCa       <  1:1858006/140‑1 (MQ=255)
                                                                                                                          cGGCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCAt      <  2:673866/140‑1 (MQ=255)
                                                                                                                            gCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCATAc    >  2:542743/1‑140 (MQ=255)
                                                                                                                            gCCACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCATAc    >  1:1080285/1‑140 (MQ=255)
                                                                                                                             ccACAGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCATACa   <  1:2162910/140‑1 (MQ=255)
                                                                                                                              cacaGCGCCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCATACAt  <  1:1518379/140‑1 (MQ=255)
                                                                                                                                   cgcCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCATACa   <  2:1490994/134‑1 (MQ=255)
                                                                                                                                   cgcCATACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCATACa   >  1:1490994/1‑134 (MQ=255)
                                                                                                                                        |                                                                                                                                 
TCGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGCGTAACGGCTCACCCCCATCAGCATCCCACCTGAAATGGTCGCCCCGGAACGGGAGAAACCCGGCCACAGCGCCAGACACTGGAAACAGCCAATCATAAATGCCTGACGATAGGTCATATCATCAAGACCCGGCGCACGCGGCTCTTTCGGCTTCAGGCATTCGGCGGCAATCAGCAACAAACCGCCAACGACCAGCGCATACAT  >  NC_000913/3203500‑3203765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A