Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F48 I1 R1	52	61.9	2103308	98.8%	2078068	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,382,176	G→T	A48D (GCT→GAT)	frdA ←	fumarate reductase flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,382,176	0	G	T	100.0%	157.6 / NA	46	A48D (GCT→GAT)	frdA	fumarate reductase flavoprotein subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (27/19); total (27/19)

GTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAG  >  NC_000913/4382044‑4382310

gTTGGGCAGTGGGGGACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGt                                                                                                                                 <  1:208434/140‑1 (MQ=255)

 ttGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTa                                                                                                                                <  2:38906/140‑1 (MQ=255)

           gtggACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCa                                                                                                                      <  2:938084/140‑1 (MQ=255)

           gtggACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCa                                                                                                                      <  2:477856/140‑1 (MQ=255)

           gtggACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGAGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCa                                                                                                                      <  2:722375/140‑1 (MQ=255)

              gACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCg                                                                                                                   >  1:264669/1‑140 (MQ=255)

              gACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCg                                                                                                                   >  1:713359/1‑140 (MQ=255)

                 aaaaTAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGt                                                                                                                <  2:10065/139‑1 (MQ=255)

                  aaaTAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGta                                                                                                               <  2:907144/140‑1 (MQ=255)

                  aaaTAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGta                                                                                                               <  2:883340/140‑1 (MQ=255)

                    ataatCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGtata                                                                                                             <  1:969197/140‑1 (MQ=255)

                    ataatCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGtata                                                                                                             <  2:726874/140‑1 (MQ=255)

                    ataatCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGCtata                                                                                                             <  1:697781/140‑1 (MQ=255)

                             gACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTgaga                                                                                                    >  1:303744/1‑140 (MQ=255)

                                   cTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTg                                                                                              <  1:389746/140‑1 (MQ=255)

                                                  gTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCg                                                                               >  1:508371/1‑140 (MQ=255)

                                                   tCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCgg                                                                              >  1:811454/1‑140 (MQ=255)

                                                       cACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCTTGCGCGACAGCGGCGGAGACCCCTTCTGCATCAACGGTATTGCTACGCATCGGGTATACTTTTGAGATTAGAGCGATTTTTGCATTCGGAttt                                                                          >  1:437742/1‑140 (MQ=255)

                                                       cACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGAttt                                                                          >  1:584423/1‑140 (MQ=255)

                                                             cTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTgc                                                                    >  2:860643/1‑140 (MQ=255)

                                                                   tATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGgca                                                              >  1:174040/1‑140 (MQ=255)

                                                                      cGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGgcagca                                                           >  1:916911/1‑140 (MQ=255)

                                                                        tGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAAt                                                         >  2:118205/1‑140 (MQ=255)

                                                                        tGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAAt                                                         >  1:289282/1‑140 (MQ=255)

                                                                           aaGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTgc                                                      <  2:811454/140‑1 (MQ=255)

                                                                            aGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTgca                                                     <  2:1046378/140‑1 (MQ=255)

                                                                            aGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTgca                                                     >  1:148473/1‑140 (MQ=255)

                                                                                 tatTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACg                                                <  2:174040/140‑1 (MQ=255)

                                                                                  atTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCTTCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGt                                               >  2:38857/1‑140 (MQ=255)

                                                                                           tGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCcgcg                                      >  1:594430/1‑140 (MQ=255)

                                                                                             tCATGATCCTGCGCGACAGCGGCGTATCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGcc                                    >  2:64234/1‑140 (MQ=255)

                                                                                               aTGATCCTGCGCGACAGCGGCGGATCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCAc                                  >  1:936627/1‑140 (MQ=255)

                                                                                               aTGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCAc                                  <  2:916911/140‑1 (MQ=255)

                                                                                                    ccTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGTCACCGgcg                             <  1:863776/140‑1 (MQ=255)

                                                                                                    ccTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGgcg                             >  2:536727/1‑140 (MQ=255)

                                                                                                    ccTGCGCGACAGCGGCGGAGCCCCCCTCTGCATCAACGGTATGGCTACGCATCGGGGATACTTTTGAGGTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGgcc                             >  2:795906/1‑139 (MQ=255)

                                                                                                     cTGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGgcgc                            >  2:45525/1‑140 (MQ=255)

                                                                                                      tGCGCGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGcc                           >  2:845412/1‑140 (MQ=255)

                                                                                                       gcgcGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCt                          >  2:285302/1‑140 (MQ=255)

                                                                                                       gcgcGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCt                          >  1:345733/1‑140 (MQ=255)

                                                                                                       gcgcGACAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCt                          >  2:566190/1‑140 (MQ=255)

                                                                                                             cAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATGGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATg                    >  2:620615/1‑140 (MQ=255)

                                                                                                             cAGCGGCGGAGCCCCCTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATg                    >  2:791807/1‑140 (MQ=255)

                                                                                                                          cccTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCtt       >  2:800551/1‑140 (MQ=255)

                                                                                                                            cTTCTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGa     <  1:860643/140‑1 (MQ=255)

                                                                                                                               cTGCATCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAg  <  1:800551/140‑1 (MQ=255)

GTTGGGCAGTGGTGGACGAAATAATCCACGACATCCTGCTCACACAACCAGTCGCCACCCGCTACTGTATCGTGAAAGTGATATTCGAAGCTGTCATGATCCTGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAG  >  NC_000913/4382044‑4382310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A