Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F48 I1 R1	75	141.5	4784044	99.1%	4740987	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,145,058	G→T	A28D (GCT→GAT)	hybA ←	hydrogenase 2 iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,145,058	0	G	T	100.0%	306.5 / NA	92	A28D (GCT→GAT)	hybA	hydrogenase 2 iron‑sulfur protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (44/48); total (44/48)

Only 100 of 102 total aligned reads displayed.

ACCAGGTCTGTTCCCCTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTT  >  NC_000913/3144921‑3145192

aCCAGGTCTGTTCCCCTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTtcatca                                                                                                                                      >  1:1504213/1‑140 (MQ=255)

     gTCTGTTCCCCTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGc                                                                                                                                 <  1:1427274/140‑1 (MQ=255)

      tCTGTTCCCCTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCa                                                                                                                                <  2:643977/140‑1 (MQ=255)

              ccTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCAtgactgac                                                                                                                        <  2:1105359/140‑1 (MQ=255)

               cTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACa                                                                                                                       >  1:503538/1‑140 (MQ=255)

               cTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACa                                                                                                                       >  2:1513658/1‑140 (MQ=255)

               cTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACa                                                                                                                       >  1:1802201/1‑140 (MQ=255)

               cTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACa                                                                                                                       >  2:1243825/1‑140 (MQ=255)

               cTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCATCAGCCGCATGACTGACa                                                                                                                       >  1:521373/1‑140 (MQ=255)

               cTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACACAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCATCAGCCGCATGACTGACa                                                                                                                       >  1:222935/1‑140 (MQ=255)

                    gggTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACgg                                                                                                                  >  1:1670228/1‑140 (MQ=255)

                    gggTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACgg                                                                                                                  >  2:1442312/1‑140 (MQ=255)

                    gggTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCATCAGCCGCATGACTGACAGACgg                                                                                                                  >  2:6291/1‑140 (MQ=255)

                         aCGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAgcg                                                                                                             <  2:2305394/140‑1 (MQ=255)

                           gTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAgcgcg                                                                                                           <  2:2165708/140‑1 (MQ=255)

                                gggAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGt                                                                                                      >  1:71467/1‑140 (MQ=255)

                                gggAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGt                                                                                                      >  1:1770298/1‑140 (MQ=255)

                                gggAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGt                                                                                                      <  2:1504213/140‑1 (MQ=255)

                                 ggAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTc                                                                                                     >  2:1973385/1‑140 (MQ=255)

                                          tatCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGcc                                                                                            <  2:555525/140‑1 (MQ=255)

                                             ccTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGccccc                                                                                         >  2:1829926/1‑139 (MQ=255)

                                             ccTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCg                                                                                         >  2:713055/1‑140 (MQ=255)

                                               tGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCaa                                                                                                <  2:1231839/131‑1 (MQ=255)

                                               tGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCaa                                                                                                >  1:1231839/1‑131 (MQ=255)

                                               tGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCaa                                                                                                >  1:68950/1‑131 (MQ=255)

                                               tGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCaa                                                                                                <  2:68950/131‑1 (MQ=255)

                                               tGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCa                                                                                       >  2:239950/1‑140 (MQ=255)

                                               tGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCCCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATTccccccc                                                                                       >  2:681054/1‑137 (MQ=255)

                                                gACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCATCTGCCGCATGACTGACAGACGGCAGCGGGCCCGTTATCAATGCCCCCCat                                                                                      >  2:1103991/1‑139 (MQ=255)

                                                 acacTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCagg                                                                                     >  1:1096452/1‑140 (MQ=255)

                                                   acTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCaggag                                                                                   <  1:314868/140‑1 (MQ=255)

                                                   acTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCaggag                                                                                   <  2:71467/140‑1 (MQ=255)

                                                     ttGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCCTCCGCCGCCTGACTGACAGACGGCAGCGCGCCCCTCAGCAAAGCCCCGCAGGAGgc                                                                                 >  2:2364023/1‑140 (MQ=255)

                                                       ggTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATg                                                                                              <  2:1652956/125‑1 (MQ=255)

                                                       ggTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATg                                                                                              >  1:1652956/1‑125 (MQ=255)

                                                       ggTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGgctg                                                                               >  1:1213686/1‑140 (MQ=255)

                                                       ggTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGgctg                                                                               >  1:2102269/1‑140 (MQ=255)

                                                       ggTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGgctg                                                                               >  1:1719769/1‑140 (MQ=255)

                                                        gTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTTATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGgctgc                                                                              >  1:1381313/1‑140 (MQ=255)

                                                             gCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTtaa                                                                         >  1:2301744/1‑140 (MQ=255)

                                                                        aGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGt                                                              <  2:1802201/140‑1 (MQ=255)

                                                                        aGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGt                                                              <  2:215652/140‑1 (MQ=255)

                                                                        aGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGt                                                              <  2:1435226/140‑1 (MQ=255)

                                                                        aGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGt                                                              <  2:1427901/140‑1 (MQ=255)

                                                                        aGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGt                                                              <  1:49288/140‑1 (MQ=255)

                                                                          ccTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCt                                                            >  1:2336292/1‑140 (MQ=255)

                                                                           cTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTg                                                           >  1:1968358/1‑140 (MQ=255)

                                                                             aCGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGtt                                                         <  2:454896/140‑1 (MQ=255)

                                                                             aCGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGtt                                                         <  1:252817/140‑1 (MQ=255)

                                                                               gCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCa                                                       <  2:1670228/140‑1 (MQ=255)

                                                                               gCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCa                                                       <  2:1244894/140‑1 (MQ=255)

                                                                               gCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCa                                                       <  2:503538/140‑1 (MQ=255)

                                                                                    aGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGtt                                                  >  1:2068261/1‑140 (MQ=255)

                                                                                     ggTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTTTTCACGGttt                                                 >  2:1572072/1‑139 (MQ=255)

                                                                                       tCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTAtt                                               <  2:2301744/140‑1 (MQ=255)

                                                                                       tCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTAtt                                               <  1:704388/140‑1 (MQ=255)

                                                                                       tCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTAtt                                               <  1:239950/140‑1 (MQ=255)

                                                                                         gAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATtct                                             >  2:1733566/1‑140 (MQ=255)

                                                                                              gTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCg                                        <  2:521373/140‑1 (MQ=255)

                                                                                                 caacaTCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCATCAGCCGCATGACTGACAGACGGCAGCTCGCCCGTCAGCAAAGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTTTTCACGGGTATTCTCCCCGttc                                     >  2:2039696/1‑138 (MQ=255)

                                                                                                 aaacaTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGa                                     <  2:1096452/139‑1 (MQ=255)

                                                                                                  aacaTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAg                                    <  2:1736744/140‑1 (MQ=255)

                                                                                                   acaTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGTATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGt                                   <  2:1968358/140‑1 (MQ=255)

                                                                                                      tCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAg                                >  1:145433/1‑140 (MQ=255)

                                                                                                      tCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAg                                <  2:1719769/140‑1 (MQ=255)

                                                                                                      tCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAg                                <  1:1243825/140‑1 (MQ=255)

                                                                                                       ccccAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGc                               >  1:1826653/1‑140 (MQ=255)

                                                                                                       ccccAGCGATCCCGGAATTGGCGGGCGGGTTTCATCAGCCGCATGACTGACAGATGGCAGGGCCCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGATATTCTCCCCGTGAGTCAgt                               >  2:823437/1‑139 (MQ=255)

                                                                                                        cccAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTTTTCACGGTTATTCTCCCCGTGAGTCAGCg                              >  1:6615/1‑140 (MQ=255)

                                                                                                        cccAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCg                              <  1:1103991/140‑1 (MQ=255)

                                                                                                        cccAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCg                              <  1:1513658/140‑1 (MQ=255)

                                                                                                         ccAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGt                             >  1:899890/1‑140 (MQ=255)

                                                                                                         ccAGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGt                             >  2:2332706/1‑140 (MQ=255)

                                                                                                           aGCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTa                           <  2:771310/140‑1 (MQ=255)

                                                                                                            gCGATCCCGGAATTGGCGGGCGGTTTTTATCAGCCGCATGACTCACAGACGGCATCGCGCCCGTCAGAAATGCCCCGCAGGAGGCTGCTTTTATAAAATTACGTTTGTTTACTGTTTTTCTCCCCGTGAGTCTTCGTTAt                          >  2:786164/1‑140 (MQ=255)

                                                                                                            gCGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTAt                          <  1:6291/140‑1 (MQ=255)

                                                                                                             cGATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTAtc                         <  2:211404/140‑1 (MQ=255)

                                                                                                              gATCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTAtct                        >  2:1096282/1‑140 (MQ=255)

                                                                                                               aTCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTAtctt                       >  2:2300056/1‑140 (MQ=255)

                                                                                                               aTCCCGGAATTGGCGGGCGGGTTTCATCAGCCGCATGACTGACCGACAGCAATGCCCCCCTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTCTTCACGGTTATTCTCCCCGTTAGACAGAGTTAtttt                       >  2:163845/1‑140 (MQ=255)

                                                                                                                tCCCGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTAtcttt                      <  2:1770298/140‑1 (MQ=255)

                                                                                                                 cccGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTAtctttc                     >  1:1922570/1‑140 (MQ=255)

                                                                                                                   cGGAATTGGCGGGCGGTTTTCATCCGCCGCATGACTGACAGACGGCCGCGCGCCCGGCAGCAATGCCCCGCAGGAGGCTGCTTTAATTAAATTACGACTGTTCACGGTTATTCTTCCCGCGAGGCTGTGTTTtctttctt                   >  2:2021728/1‑140 (MQ=255)

                                                                                                                   cGGAATTGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTAtctttctt                   <  1:681054/140‑1 (MQ=255)

                                                                                                                       aTTGGCGGGCGGGTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGc               >  1:1827972/1‑140 (MQ=255)

                                                                                                                        ttGGCGGGCGGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCt              <  1:2021728/140‑1 (MQ=255)

                                                                                                                          ggcgggcgGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTgt            >  2:286578/1‑139 (MQ=255)

                                                                                                                          ggcgggcgGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTga            >  1:1386844/1‑140 (MQ=255)

                                                                                                                          ggcgggcgGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTga            >  2:1515627/1‑140 (MQ=255)

                                                                                                                          ggcgggcgGTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTga            >  2:798231/1‑140 (MQ=255)

                                                                                                                           gcgggcggTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTgac           >  1:1375033/1‑140 (MQ=255)

                                                                                                                           gcgggcggTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTgac           >  2:1277200/1‑140 (MQ=255)

                                                                                                                              ggcggTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTgacgac        <  1:1277200/140‑1 (MQ=255)

                                                                                                                              ggcggTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCACCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTACCCGTGAGTCAGCGTTATCTTACTTTTGCTgacgac        <  1:786164/140‑1 (MQ=255)

                                                                                                                               gcggTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGAcc       <  2:540287/140‑1 (MQ=255)

                                                                                                                                cggTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGAccc      <  1:713055/140‑1 (MQ=255)

                                                                                                                                cggTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGAccc      <  1:798231/140‑1 (MQ=255)

                                                                                                                                cggTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGAccc      <  2:851282/140‑1 (MQ=255)

                                                                                                                                  gTTTTCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTC‑TTTGCTGACGACCCAGt   <  1:1557772/140‑1 (MQ=255)

                                                                                                                                    tttCATCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGtt  <  2:1827972/140‑1 (MQ=255)

ACCAGGTCTGTTCCCCTTGCGGGTTACGTTCAGGGAAATTGATATCCTGACACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTT  >  NC_000913/3144921‑3145192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A