Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F48 I1 R1	75	141.5	4784044	99.1%	4740987	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,853	A→G	K5R (AAA→AGA)	ubiK →	ubiquinone biosynthesis accessory factor UbiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,853	0	A	G	100.0%	620.4 / NA	155	K5R (AAA→AGA)	ubiK	ubiquinone biosynthesis accessory factor UbiK
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (97/58); total (97/58)

Only 100 of 158 total aligned reads displayed.

AGCGGATTCACTATAACGCTAATGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGA  >  NC_000913/3184716‑3184988

    gATTCACTATAACGCTAATGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAAt                                                                                                                                   >  2:1073742/1‑140 (MQ=255)

      ttCACTATAACGCTAATGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTg                                                                                                                                 >  1:1217659/1‑140 (MQ=255)

                    aaTGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACATGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAAtcgctcgc                                                                                                                   <  2:528100/140‑1 (MQ=255)

                    aaTGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAAtcgctcgc                                                                                                                   >  1:2224610/1‑140 (MQ=255)

                     aTGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGcc                                                                                                                  >  2:92550/1‑140 (MQ=255)

                     aTGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGcc                                                                                                                  <  1:212606/140‑1 (MQ=255)

                     aTGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGcc                                                                                                                  >  1:2125369/1‑140 (MQ=255)

                     aTGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGcc                                                                                                                  >  1:1838756/1‑140 (MQ=255)

                       gATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAg                                                                                                                >  1:1084929/1‑140 (MQ=255)

                          tAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGtt                                                                                                             >  1:1958026/1‑140 (MQ=255)

                           aGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTc                                                                                                            >  2:567492/1‑140 (MQ=255)

                            gCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCa                                                                                                           <  2:1217659/140‑1 (MQ=255)

                            gCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCa                                                                                                           <  2:1838756/140‑1 (MQ=255)

                            gCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCa                                                                                                           >  1:287146/1‑140 (MQ=255)

                            gCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCa                                                                                                           <  2:1988897/140‑1 (MQ=255)

                             cGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCAc                                                                                                          >  2:597586/1‑140 (MQ=255)

                                 agcaACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAACGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAAt                                                                                                      <  1:182083/140‑1 (MQ=255)

                                  gcaACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATc                                                                                                     >  2:157154/1‑140 (MQ=255)

                                   caACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCa                                                                                                    <  2:1285742/140‑1 (MQ=255)

                                   caACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCa                                                                                                    <  2:194169/140‑1 (MQ=255)

                                   aaaCGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCa                                                                                                    <  2:255836/139‑1 (MQ=255)

                                          tAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTa                                                                                             <  2:1776086/140‑1 (MQ=255)

                                           aGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCTGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTaa                                                                                            <  1:947234/140‑1 (MQ=255)

                                           aGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTaa                                                                                            <  2:287146/140‑1 (MQ=255)

                                           aGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTaa                                                                                            <  1:597586/140‑1 (MQ=255)

                                             cTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAg                                                                                          >  1:1256135/1‑140 (MQ=255)

                                               tCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGa                                                                                        <  1:852745/140‑1 (MQ=255)

                                                cacaTAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGaa                                                                                       >  2:1345059/1‑140 (MQ=255)

                                                cacaTAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGaa                                                                                       >  1:1491255/1‑140 (MQ=255)

                                                  caTAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATc                                                                                     >  2:810801/1‑140 (MQ=255)

                                                     aaTTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCCCTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAgg                                                                                  <  2:2140792/140‑1 (MQ=255)

                                                     aaTTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAGACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAgg                                                                                  <  1:282987/140‑1 (MQ=255)

                                                     aaTTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAgg                                                                                  <  2:2296870/140‑1 (MQ=255)

                                                     aaTTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAgg                                                                                  <  2:876518/140‑1 (MQ=255)

                                                      aTTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAggg                                                                                 <  2:340315/140‑1 (MQ=255)

                                                       ttCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAgggg                                                                                >  1:1745365/1‑139 (MQ=255)

                                                       ttCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGa                                                                                >  2:108064/1‑140 (MQ=255)

                                                       ttCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGa                                                                                >  1:235670/1‑140 (MQ=255)

                                                          tGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGtt                                                                             <  2:1335407/140‑1 (MQ=255)

                                                                 tGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGaa                                                                      >  1:1600723/1‑140 (MQ=255)

                                                                 tGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGaa                                                                      >  2:146520/1‑140 (MQ=255)

                                                                 tGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGaa                                                                      >  1:1420673/1‑140 (MQ=255)

                                                                      tACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGAtgt                                                                 >  2:561694/1‑140 (MQ=255)

                                                                       aCCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGAtgtg                                                                >  2:444927/1‑140 (MQ=255)

                                                                            tATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGaa                                                           >  2:380146/1‑140 (MQ=255)

                                                                             aTCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGCGTTCGGGGAAGATGTGGAGaaa                                                          >  1:1477575/1‑140 (MQ=255)

                                                                               cGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGaaaaa                                                        >  2:1490575/1‑140 (MQ=255)

                                                                                  aCTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAAt                                                     >  1:2169702/1‑140 (MQ=255)

                                                                                   cTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGTAAAAAATc                                                    >  1:1354806/1‑140 (MQ=255)

                                                                                   cTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATc                                                    >  2:772141/1‑140 (MQ=255)

                                                                                   cTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATc                                                    >  1:941081/1‑140 (MQ=255)

                                                                                   cTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATc                                                    >  2:1753178/1‑140 (MQ=255)

                                                                                        aTGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCGCGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCa                                               <  2:2169702/140‑1 (MQ=255)

                                                                                        aTGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGGGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCa                                               <  2:1745365/140‑1 (MQ=255)

                                                                                        aTGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCa                                               <  2:941081/140‑1 (MQ=255)

                                                                                        aTGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCa                                               <  2:492951/140‑1 (MQ=255)

                                                                                          ggCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCaaa                                             <  2:1705954/140‑1 (MQ=255)

                                                                                           gCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAAc                                            >  1:1146753/1‑140 (MQ=255)

                                                                                              cTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCt                                         >  1:1541372/1‑140 (MQ=255)

                                                                                              cTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCt                                         >  2:1129485/1‑140 (MQ=255)

                                                                                              cTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCt                                         >  1:1912696/1‑140 (MQ=255)

                                                                                              cTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGCTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCt                                         >  1:2068999/1‑140 (MQ=255)

                                                                                                cAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCa                                               <  2:1167550/132‑1 (MQ=255)

                                                                                                cAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCa                                               >  1:1167550/1‑132 (MQ=255)

                                                                                                cAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTAc                                       >  1:1049840/1‑140 (MQ=255)

                                                                                                 aaCACCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACa                                      >  1:201251/1‑140 (MQ=255)

                                                                                                   cacCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAg                                    <  1:50577/140‑1 (MQ=255)

                                                                                                   cacCTATCAGTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAg                                    >  1:340962/1‑140 (MQ=255)

                                                                                                            gTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGa                           <  1:108064/140‑1 (MQ=255)

                                                                                                            gTACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGa                           <  2:1108146/140‑1 (MQ=255)

                                                                                                             tACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAc                          >  1:80085/1‑140 (MQ=255)

                                                                                                             tACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAc                          <  1:2012957/140‑1 (MQ=255)

                                                                                                             tACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAc                          >  2:1328682/1‑140 (MQ=255)

                                                                                                             tACAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAc                          >  1:2117901/1‑140 (MQ=255)

                                                                                                              aCAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAcg                         <  2:1974361/140‑1 (MQ=255)

                                                                                                               cAGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCACGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAcgc                        >  2:1594230/1‑140 (MQ=255)

                                                                                                                aGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAcgcg                       >  1:1578363/1‑140 (MQ=255)

                                                                                                                aGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGAcgcg                       >  1:1264817/1‑140 (MQ=255)

                                                                                                                aGGGAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGAGCAGCTGAcgcg                       >  1:274863/1‑140 (MQ=255)

                                                                                                                  ggAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGcc                     >  1:1058207/1‑140 (MQ=255)

                                                                                                                  ggAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGcc                     >  1:1895420/1‑140 (MQ=255)

                                                                                                                  ggAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGcc                     >  1:769194/1‑140 (MQ=255)

                                                                                                                   gAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCt                    >  1:292119/1‑140 (MQ=255)

                                                                                                                   gAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCt                    <  2:1869596/140‑1 (MQ=255)

                                                                                                                   gAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCt                    <  2:1250650/140‑1 (MQ=255)

                                                                                                                   gAAACTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCCGACGCGCCt                    <  2:1541372/140‑1 (MQ=255)

                                                                                                                     aaCTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCg                  >  1:1215743/1‑140 (MQ=255)

                                                                                                                     aaCTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCg                  <  2:1215743/140‑1 (MQ=255)

                                                                                                                     aaCTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCg                  <  1:174548/140‑1 (MQ=255)

                                                                                                                     aaCTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCg                  <  1:380146/140‑1 (MQ=255)

                                                                                                                       cTACAATGATTGACCCGAGAAAACTTGACCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAACATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                >  2:517871/1‑140 (MQ=255)

                                                                                                                       cTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                >  2:2056712/1‑140 (MQ=255)

                                                                                                                       cTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                <  1:391040/140‑1 (MQ=255)

                                                                                                                       cTACAATGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAc                >  1:897163/1‑140 (MQ=255)

                                                                                                                             tGATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTa          >  1:469170/1‑140 (MQ=255)

                                                                                                                              gATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTaa         >  1:1657562/1‑140 (MQ=255)

                                                                                                                              gATTGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACG‑ATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAg        >  1:912368/1‑140 (MQ=255)

                                                                                                                                 tGACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGcc      <  2:291953/140‑1 (MQ=255)

                                                                                                                                  gACCCGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCg     >  2:2090393/1‑140 (MQ=255)

                                                                                                                                    cccGAGAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGTGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTg   >  1:1131223/1‑140 (MQ=255)

AGCGGATTCACTATAACGCTAATGATTAGCGGCAGCAACGCATAGCTTCACATAATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGA  >  NC_000913/3184716‑3184988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A