Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F48 I1 R1	75	141.5	4784044	99.1%	4740987	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,447,015	C→A	D142Y (GAC→TAC)	rplE ←	50S ribosomal subunit protein L5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,447,015	0	C	A	98.7%	552.5 / ‑2.2	158	D142Y (GAC→TAC)	rplE	50S ribosomal subunit protein L5
Reads supporting (aligned to +/- strand): ref base C (1/1); major base A (77/77); minor base G (1/1); total (79/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 161 total aligned reads displayed.

CCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCA  >  NC_000913/3446881‑3447150

   tttAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTg                                                                                                                                 <  2:344508/140‑1 (MQ=255)

   tttAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTg                                                                                                                                 <  1:879018/140‑1 (MQ=255)

     tAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTggg                                                                                                                               >  2:618132/1‑140 (MQ=255)

      aGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGa                                                                                                                              <  1:383419/140‑1 (MQ=255)

          aCCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgat                                                                                                                          >  2:1547172/1‑140 (MQ=255)

          aCCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgat                                                                                                                          >  1:64772/1‑140 (MQ=255)

          aCCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgat                                                                                                                          >  2:182497/1‑140 (MQ=255)

          aCCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgat                                                                                                                          >  1:1577552/1‑140 (MQ=255)

             cTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgatgat                                                                                                                       <  2:1700236/140‑1 (MQ=255)

             cTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgatgat                                                                                                                       <  1:1005704/140‑1 (MQ=255)

             cTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgatgat                                                                                                                       <  1:284714/140‑1 (MQ=255)

             cTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgatgat                                                                                                                       <  2:1427905/140‑1 (MQ=255)

             cTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgatgat                                                                                                                       <  2:1972112/140‑1 (MQ=255)

             cTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAgatgat                                                                                                                       <  1:1409585/140‑1 (MQ=255)

              tACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATc                                                                                                                      >  2:1379312/1‑140 (MQ=255)

               aCCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCt                                                                                                                     >  2:428959/1‑140 (MQ=255)

               aCCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCt                                                                                                                     >  1:185738/1‑140 (MQ=255)

                ccTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTg                                                                                                                    >  2:244346/1‑140 (MQ=255)

                ccTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTg                                                                                                                    >  2:1952638/1‑140 (MQ=255)

                  ttACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCt                                                                                                                  >  2:2259433/1‑140 (MQ=255)

                  ttACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCt                                                                                                                  >  2:372898/1‑140 (MQ=255)

                   tACTTGCGGAACGGGAAGTCAAAGGCAGCCAGTAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTc                                                                                                                 >  2:1101760/1‑140 (MQ=255)

                    aCTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCa                                                                                                                <  2:38395/140‑1 (MQ=255)

                     cTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCAc                                                                                                               >  2:2023750/1‑140 (MQ=255)

                     cTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGACCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTGGATTTCTGGGAAGATGATCTGCTCAc                                                                                                               >  2:1932601/1‑140 (MQ=255)

                      ttGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACg                                                                                                              <  1:2378763/140‑1 (MQ=255)

                      ttGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACg                                                                                                              >  2:1338991/1‑140 (MQ=255)

                      ttGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACg                                                                                                              >  1:992843/1‑140 (MQ=255)

                      ttGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACg                                                                                                              >  1:956994/1‑140 (MQ=255)

                        gCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGa                                                                                                            <  2:185738/140‑1 (MQ=255)

                         cGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGac                                                                                                           >  1:1462442/1‑140 (MQ=255)

                          ggAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGaca                                                                                                          >  1:1793555/1‑140 (MQ=255)

                          ggAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGaca                                                                                                          >  1:1628372/1‑140 (MQ=255)

                          ggAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGaca                                                                                                          >  2:893598/1‑140 (MQ=255)

                           gAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGacac                                                                                                         >  2:31961/1‑140 (MQ=255)

                           gAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGAacac                                                                                                         <  1:2259433/140‑1 (MQ=255)

                               gggAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCAt                                                                                                     <  1:24909/140‑1 (MQ=255)

                                ggAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATg                                                                                                    <  1:2023750/140‑1 (MQ=255)

                                   aGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTg                                                                                                 >  1:554665/1‑140 (MQ=255)

                                   aGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTg                                                                                                 <  1:243724/140‑1 (MQ=255)

                                     tCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTa                                                                                               <  1:1011347/140‑1 (MQ=255)

                                     tCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTa                                                                                               <  1:428959/140‑1 (MQ=255)

                                             agccagcAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCAc                                                                                       <  2:675565/140‑1 (MQ=255)

                                              gccagcAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACg                                                                                      >  2:111749/1‑140 (MQ=255)

                                                cagcagAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGAc                                                                                    <  1:356108/140‑1 (MQ=255)

                                                cagcagAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGAc                                                                                    <  1:1767868/140‑1 (MQ=255)

                                                       gcgcGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGaa                                                                             >  1:367411/1‑140 (MQ=255)

                                                        cgcgGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGaaa                                                                            >  1:530172/1‑140 (MQ=255)

                                                                   tcGTCCGATTCCGCAGAAGGCGTAATAGAAATATCCAAACAAAGAACGCGGTCGACTTTATCGTAATAGATTTCTGGGAAGATGATCAGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACATAGCGGa                                                                 <  2:695591/140‑1 (MQ=255)

                                                                   tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGa                                                                 <  2:1536807/140‑1 (MQ=255)

                                                                   tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGa                                                                 <  1:1952638/140‑1 (MQ=255)

                                                                   tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGa                                                                 <  2:713322/140‑1 (MQ=255)

                                                                   tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGa                                                                 <  1:480479/140‑1 (MQ=255)

                                                                   tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGa                                                                 <  2:956994/140‑1 (MQ=255)

                                                                   tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGa                                                                 <  1:1109322/140‑1 (MQ=255)

                                                                   tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGa                                                                 <  1:618132/140‑1 (MQ=255)

                                                                   tcGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGa                                                                 <  1:893598/140‑1 (MQ=255)

                                                                                    agtGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCAc                                                <  1:1338991/140‑1 (MQ=255)

                                                                                        gtaatggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGAt                                            >  2:2134536/1‑140 (MQ=255)

                                                                                         taatggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATa                                           <  1:2258371/140‑1 (MQ=255)

                                                                                          aatggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATAc                                          >  1:421352/1‑140 (MQ=255)

                                                                                           atggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACg                                         <  1:1547172/140‑1 (MQ=255)

                                                                                            tggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCCCGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGa                                        <  1:2134536/140‑1 (MQ=255)

                                                                                             ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGat                                       >  2:1925508/1‑139 (MQ=255)

                                                                                             ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAg                                       >  2:877030/1‑140 (MQ=255)

                                                                                             ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAg                                       >  2:476823/1‑140 (MQ=255)

                                                                                             ggtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACAACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAg                                       >  1:167565/1‑140 (MQ=255)

                                                                                              gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACg                                         <  2:870857/137‑1 (MQ=255)

                                                                                              gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAgg                                      <  1:773260/140‑1 (MQ=255)

                                                                                              gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAgg                                      <  2:553355/140‑1 (MQ=255)

                                                                                              gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAgg                                      <  2:1175490/140‑1 (MQ=255)

                                                                                              gtaatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAgg                                      <  2:421352/140‑1 (MQ=255)

                                                                                               taatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGt                                     <  2:1577552/140‑1 (MQ=255)

                                                                                                aatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAggaa                                    >  2:223136/1‑138 (MQ=255)

                                                                                                aatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAgg                                      >  1:1508231/1‑138 (MQ=255)

                                                                                                aatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAgg                                      <  2:1508231/138‑1 (MQ=255)

                                                                                                aatATCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTa                                    >  2:1849033/1‑140 (MQ=255)

                                                                                                 atatCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTa                                    >  2:2068921/1‑139 (MQ=255)

                                                                                                  tatCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACa                                  >  1:270486/1‑140 (MQ=255)

                                                                                                  tatCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACa                                  >  2:597121/1‑140 (MQ=255)

                                                                                                   atCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAg                                 <  2:530172/140‑1 (MQ=255)

                                                                                                   atCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAg                                 <  2:986955/140‑1 (MQ=255)

                                                                                                   atCCAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAg                                 <  1:1101760/140‑1 (MQ=255)

                                                                                                      cAAACCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCaa                              <  2:1462442/140‑1 (MQ=255)

                                                                                                       aaaCCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAAt                             >  1:979289/1‑140 (MQ=255)

                                                                                                        aaCCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACg                                         >  2:930992/1‑127 (MQ=255)

                                                                                                        aaCCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACg                                         <  1:930992/127‑1 (MQ=255)

                                                                                                        aaCCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATa                            >  1:1225471/1‑140 (MQ=255)

                                                                                                        aaCCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATa                            >  1:157196/1‑140 (MQ=255)

                                                                                                        aaCCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATa                            >  1:1296066/1‑140 (MQ=255)

                                                                                                         aCCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGCAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAATTCACGGATACGAGGTACAGCAATAg                           >  1:680559/1‑140 (MQ=255)

                                                                                                         aCCACGAACGCGGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAg                           >  1:987716/1‑140 (MQ=255)

                                                                                                                   cgGTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGgcg                 >  1:1181583/1‑140 (MQ=255)

                                                                                                                     gTCGACTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCt               >  2:1042075/1‑140 (MQ=255)

                                                                                                                         aCTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCaaa           <  2:1745804/140‑1 (MQ=255)

                                                                                                                         aCTTTATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCaaa           <  2:277998/140‑1 (MQ=255)

                                                                                                                            ttATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACAACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGac        <  2:167565/140‑2 (MQ=255)

                                                                                                                             tATCGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAAc       >  1:1239307/1‑140 (MQ=255)

                                                                                                                                cGTAGTAGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTcc    <  2:1239307/140‑1 (MQ=255)

                                                                                                                                  tagtagATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCca  <  1:858996/140‑1 (MQ=255)

CCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTCGATTTCTGGGAAGATGATCTGCTCACGGACACCCATGCTGTAGTTACCACGACCGTCGAAAGACTTAGCGGACAGGCCACGGAAGTCACGGATACGAGGTACAGCAATAGTGATCAGGCGCTCAAAGAACTCCCA  >  NC_000913/3446881‑3447150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A