Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F48 I1 R1 75 141.5 4784044 99.1% 4740987 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,451,751 C→A S179I (AGC→ATC)  rplD ← 50S ribosomal subunit protein L4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,451,7510CA99.1% 394.2 / ‑3.0 110S179I (AGC→ATC) rplD50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (54/55);  total (55/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 110 total aligned reads displayed.

CGCAGTAGACGCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGCTAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAG  >  NC_000913/3451616‑3451887
                                                                                                                                       |                                                                                                                                        
cGCAGTAGACGCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAAc                                                                                                                                      <  2:1222309/140‑1 (MQ=255)
cGCAGTAGACGCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAAc                                                                                                                                      <  2:92234/140‑1 (MQ=255)
cGCAGTAGACGCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTACTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAAc                                                                                                                                      <  2:524768/140‑1 (MQ=255)
 gCAGTAGACGCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAAcc                                                                                                                                     <  1:977492/140‑1 (MQ=255)
   aGTAGACGCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCgg                                                                                                                                   <  1:1027786/140‑1 (MQ=255)
    gTAGACGCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTACTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCggg                                                                                                                                  <  1:885897/140‑1 (MQ=255)
         agCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGAt                                                                                                                             <  2:83613/139‑1 (MQ=255)
          gCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATa                                                                                                                            >  2:1952367/1‑140 (MQ=255)
            tttttCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATAcc                                                                                                                          >  1:760942/1‑140 (MQ=255)
             ttttCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCa                                                                                                                         <  2:760942/140‑1 (MQ=255)
             ttttCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCa                                                                                                                         >  1:1720056/1‑140 (MQ=255)
                   gAATCGTGCGGTGCACGCAGCGCCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTATCAGCATCCGTAGTCATTACGTTTTTGTCGAAGGCGATCAGTATAACCGGGTCTATACCAGTTGCa                                                                                                                   <  1:1689987/140‑1 (MQ=255)
                     aaCGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATc                                                                                                                 >  1:1057130/1‑140 (MQ=255)
                      aCGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATcg                                                                                                                >  1:1175414/1‑140 (MQ=255)
                      aCGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATcg                                                                                                                >  2:1956549/1‑140 (MQ=255)
                      aCGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATcg                                                                                                                >  2:182423/1‑140 (MQ=255)
                      aCGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATcg                                                                                                                >  2:1276794/1‑140 (MQ=255)
                        gTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATcgcg                                                                                                              <  2:322157/140‑1 (MQ=255)
                        gTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATcgcg                                                                                                              <  1:365197/140‑1 (MQ=255)
                        gTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAACAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATcgcg                                                                                                              <  1:1060134/140‑1 (MQ=255)
                             gTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGt                                                                                                         <  2:274478/140‑1 (MQ=255)
                             gTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGt                                                                                                         <  2:1654809/140‑1 (MQ=255)
                             gTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGt                                                                                                         <  2:1761792/140‑1 (MQ=255)
                              tGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTc                                                                                                        >  2:177543/1‑140 (MQ=255)
                                cACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCaa                                                                                                      >  1:1909361/1‑140 (MQ=255)
                                 aCGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAAc                                                                                                     <  2:2217336/140‑1 (MQ=255)
                                   gcagcaCCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCt                                                                                                   <  2:2095053/140‑1 (MQ=255)
                                       caCCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTtgtg                                                                                               <  1:182423/140‑1 (MQ=255)
                                        aCCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGc                                                                                              >  2:664465/1‑140 (MQ=255)
                                            tCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGt                                                                                          >  1:1818042/1‑140 (MQ=255)
                                            tCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGt                                                                                          >  1:834087/1‑140 (MQ=255)
                                               gcagACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAGCAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTgc                                                                                       >  2:1668487/1‑140 (MQ=255)
                                               gcagACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTgc                                                                                       >  2:2319789/1‑140 (MQ=255)
                                                  gACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTgcgcg                                                                                    <  1:1621185/140‑1 (MQ=255)
                                                  gACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTgcgcg                                                                                    <  1:1108324/140‑1 (MQ=255)
                                                     gTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAg                                                                                 >  1:2364950/1‑140 (MQ=255)
                                                      ttcttcACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGc                                                                                >  2:1364825/1‑140 (MQ=255)
                                                              gAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACa                                                                        <  1:913366/140‑1 (MQ=255)
                                                                aTCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAgg                                                                      >  2:1237248/1‑140 (MQ=255)
                                                                   aTGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGttt                                                                   >  1:472580/1‑140 (MQ=255)
                                                                     gCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTc                                                                 >  2:355270/1‑140 (MQ=255)
                                                                        aGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTc                                                              <  2:794100/140‑1 (MQ=255)
                                                                        aGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTc                                                              <  2:64815/140‑1 (MQ=255)
                                                                        aGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTc                                                              >  2:943687/1‑140 (MQ=255)
                                                                        aGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTc                                                              <  1:1364825/140‑1 (MQ=255)
                                                                        aGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTc                                                              <  1:1895870/140‑1 (MQ=255)
                                                                          cATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCa                                                            >  2:1201402/1‑140 (MQ=255)
                                                                          cATCTCCTCAACTAGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGTATAACCAGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCa                                                            >  2:717184/1‑140 (MQ=255)
                                                                           aTCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAg                                                           <  1:1035281/140‑1 (MQ=255)
                                                                           aTCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAg                                                           >  2:1926737/1‑140 (MQ=255)
                                                                            tctcCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGc                                                          >  2:471286/1‑140 (MQ=255)
                                                                             ctcctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCt                                                         <  1:1926737/140‑1 (MQ=255)
                                                                             ctcctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCt                                                         <  1:2170003/140‑1 (MQ=255)
                                                                             ctcctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCt                                                         <  2:1094444/140‑1 (MQ=255)
                                                                             ctcctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCt                                                         <  2:1383825/140‑1 (MQ=255)
                                                                               cctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCa                                                       <  2:834087/140‑1 (MQ=255)
                                                                               cctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCa                                                       >  2:12380/1‑140 (MQ=255)
                                                                                ctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCCGCTCAc                                                      <  2:2364950/140‑1 (MQ=255)
                                                                                ctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCAc                                                      <  2:22435/140‑1 (MQ=255)
                                                                                ctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCAc                                                      <  1:2025096/140‑1 (MQ=255)
                                                                                ctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCAc                                                      <  1:471286/140‑1 (MQ=255)
                                                                                ctcAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCAc                                                      <  2:1057130/140‑1 (MQ=255)
                                                                                  cAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCg                                                    <  1:1729242/140‑1 (MQ=255)
                                                                                   aaCTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGCTAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCgg                                                   >  1:458118/1‑140 (MQ=255)
                                                                                     attGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGtg                                                 <  2:1818042/139‑1 (MQ=255)
                                                                                       tGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGtgat                                               >  1:1459154/1‑140 (MQ=255)
                                                                                        gCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGtgatg                                              <  1:2319942/140‑1 (MQ=255)
                                                                                        gCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGtgatg                                              >  1:1195180/1‑140 (MQ=255)
                                                                                         cTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGtgatga                                             >  1:2332647/1‑140 (MQ=255)
                                                                                          ttAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTgatgat                                            >  1:612619/1‑140 (MQ=255)
                                                                                            aaCAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCa                                          <  1:355270/140‑1 (MQ=255)
                                                                                               aGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGca                                       <  1:1956549/140‑1 (MQ=255)
                                                                                                gCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGcac                                      >  1:1356997/1‑140 (MQ=255)
                                                                                                gCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGcac                                      >  1:264616/1‑140 (MQ=255)
                                                                                                 cATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGcaca                                     >  1:458981/1‑140 (MQ=255)
                                                                                                 cATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGcaca                                     >  1:1841224/1‑140 (MQ=255)
                                                                                                  aTCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACAt                                    >  2:1446741/1‑140 (MQ=255)
                                                                                                   tCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATc                                   >  1:1670101/1‑140 (MQ=255)
                                                                                                   tCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATc                                   >  1:2333654/1‑140 (MQ=255)
                                                                                                   tCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATc                                   >  1:2319134/1‑140 (MQ=255)
                                                                                                      acagTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTc                                <  2:1909361/139‑1 (MQ=255)
                                                                                                         gTCATTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCag                             >  2:1179004/1‑140 (MQ=255)
                                                                                                            aTTACGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGc                          >  2:485042/1‑140 (MQ=255)
                                                                                                               aCGACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCAt                       >  1:88539/1‑140 (MQ=255)
                                                                                                                 gACTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGt                     <  1:2263593/140‑1 (MQ=255)
                                                                                                                  aCTTTGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTc                    >  1:2154972/1‑140 (MQ=255)
                                                                                                                      tGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTc                >  1:299826/1‑140 (MQ=255)
                                                                                                                      tGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTc                >  2:232159/1‑140 (MQ=255)
                                                                                                                      tGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTc                >  1:294098/1‑140 (MQ=255)
                                                                                                                      tGTCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTc                >  2:871589/1‑140 (MQ=255)
                                                                                                                      tGTCGAAGGCGATCAGGATAACCGGATCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTc                >  2:1263733/1‑140 (MQ=255)
                                                                                                                        tCGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAg              >  1:1116659/1‑140 (MQ=255)
                                                                                                                         cGAAGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCC‑TGTCtcttatac            <  1:1668487/140‑8 (MQ=255)
                                                                                                                            aGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTc          <  1:445244/140‑1 (MQ=255)
                                                                                                                            aGGCGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTc          <  1:871589/140‑1 (MQ=255)
                                                                                                                               cGATCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCtgt       <  1:2319789/140‑1 (MQ=255)
                                                                                                                                  tCAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGcc    <  1:1230705/140‑1 (MQ=255)
                                                                                                                                   cAGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCca   <  1:1201402/140‑1 (MQ=255)
                                                                                                                                    aGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCcag  <  1:485042/140‑1 (MQ=255)
                                                                                                                                    aGGATAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCcag  <  1:1179004/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
CGCAGTAGACGCTTTTTCAGAAACGTGCGGTGCACGCAGCACCTTCAGCAGACGTTCTTCACGAATCATGCCAGCATCTCCTCAACTTGCTTAACAGCATCAGCAGTCATTACGACTTTGTCGAAGGCGATCAGGCTAACCGGGTCGATACCAGTTGCATCGCGTACGTCAACCTTGTGCAGGTTGCGCGCAGCCAGGAACAGGTTTTCGTCCAGCTCACCGGTGATGATCAGCACATCTTCCAGAGCCATGTCTTTCAGTTTCTGTGCCAG  >  NC_000913/3451616‑3451887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A