Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F48 I1 R1 115 87.5 3003888 99.1% 2976853 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,315,121 G→A G298G (GGC→GGT)  infB ← translation initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,315,1210GA100.0% 199.5 / NA 58G298G (GGC→GGT) infBtranslation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/23);  total (35/23)

GTTGCCATTGCGCCCAGTTTCATCATCGCTTTGATGACCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACC  >  NC_000913/3314984‑3315255
                                                                                                                                         |                                                                                                                                      
gTTGCCATTGCGCCCAGTTTCATCATCGCTTTGATGACCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAAcc                                                                                                                                      >  2:392479/1‑140 (MQ=255)
            cccAGTTTCATCATCGCTTTGATGACCTGAGAGCCTTTAACCGCTATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCga                                                                                                                          >  1:412562/1‑140 (MQ=255)
             ccAGTTTCATCATCGCTTTGATGACCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCgaa                                                                                                                         <  1:514385/140‑1 (MQ=255)
                         tcGCTTTGATGACCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGt                                                                                                             >  2:835425/1‑140 (MQ=255)
                           gCTTTGATGACCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGttt                                                                                                           >  2:725356/1‑140 (MQ=255)
                           gCTTTGATGACCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGttt                                                                                                           >  1:1312650/1‑140 (MQ=255)
                           gCTTTGATGACCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGttt                                                                                                           >  2:174435/1‑140 (MQ=255)
                             tttGATGACCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTc                                                                                                         >  2:379462/1‑140 (MQ=255)
                                   gaCCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCtt                                                                                                   <  1:1106255/140‑1 (MQ=255)
                                     ccTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTa                                                                                                 <  1:1077436/140‑1 (MQ=255)
                                     ccTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTa                                                                                                 <  1:1480824/140‑1 (MQ=255)
                                        gagaGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCg                                                                                              <  2:830731/140‑1 (MQ=255)
                                        gagaGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCg                                                                                              <  1:835425/140‑1 (MQ=255)
                                         agagCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGc                                                                                             >  1:856976/1‑140 (MQ=255)
                                         agagCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGc                                                                                             >  2:167607/1‑140 (MQ=255)
                                         agagCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGc                                                                                             >  1:251181/1‑140 (MQ=255)
                                          gagCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGcc                                                                                            <  2:1373161/140‑1 (MQ=255)
                                           agCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCa                                                                                           >  1:1039737/1‑140 (MQ=255)
                                           agCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCa                                                                                           >  2:1499452/1‑140 (MQ=255)
                                              cTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGt                                                                                        >  2:343958/1‑140 (MQ=255)
                                              cTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGt                                                                                        >  2:208641/1‑140 (MQ=255)
                                                  aaCCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTActg                                                                                    <  1:710741/140‑1 (MQ=255)
                                                       ccATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTgcg                                                                               <  1:1126542/140‑1 (MQ=255)
                                                        cATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTgcgc                                                                              <  2:877415/140‑1 (MQ=255)
                                                         aTCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGcgcg                                                                             <  1:208641/140‑1 (MQ=255)
                                                             tGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGct                                                                         <  1:167607/140‑1 (MQ=255)
                                                             tGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGct                                                                         <  1:725356/140‑1 (MQ=255)
                                                               ttcgccagttcgccaACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGcttt                                                                       >  1:449169/1‑139 (MQ=255)
                                                                 cgccagttcgccaACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGcttctt                                                                     >  1:1379428/1‑140 (MQ=255)
                                                                     agttcgccaACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACg                                                                 <  1:533518/140‑1 (MQ=255)
                                                                         cgccaACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCa                                                             >  2:669423/1‑140 (MQ=255)
                                                                                   gATATTTTCGCCGATCACAACTTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCAAGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGAtt                                                   <  1:994993/140‑1 (MQ=255)
                                                                                          tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCgtg                                            >  1:630177/1‑140 (MQ=255)
                                                                                          tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCgtg                                            >  1:154278/1‑140 (MQ=255)
                                                                                          tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCgtg                                            >  2:331867/1‑140 (MQ=255)
                                                                                                 tCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTttgttg                                     >  1:664686/1‑140 (MQ=255)
                                                                                                       cGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCtttt                               >  1:1342112/1‑139 (MQ=255)
                                                                                                          cACGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTttcttc                            >  2:266746/1‑140 (MQ=255)
                                                                                                           aCGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCg                           >  1:1088744/1‑140 (MQ=255)
                                                                                                            cGGTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCgg                          >  1:1075585/1‑140 (MQ=255)
                                                                                                              gTTAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGAc                        <  1:251755/140‑1 (MQ=255)
                                                                                                               ttAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGAcg                       <  2:1312650/140‑1 (MQ=255)
                                                                                                               ttAACGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGAcg                       >  1:1228201/1‑140 (MQ=255)
                                                                                                                 aaCGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGAcgcg                     >  2:304253/1‑140 (MQ=255)
                                                                                                                 aaCGGCCTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGAcgcg                     <  1:392479/140‑1 (MQ=255)
                                                                                                                       cTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCtt               >  1:448894/1‑140 (MQ=255)
                                                                                                                       cTGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCtt               >  1:119834/1‑140 (MQ=255)
                                                                                                                        tGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCttt              <  2:1379428/140‑1 (MQ=255)
                                                                                                                        tGAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCttt              >  2:1111736/1‑140 (MQ=255)
                                                                                                                         gAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTc             >  1:853996/1‑140 (MQ=255)
                                                                                                                         gAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTc             >  1:655250/1‑140 (MQ=255)
                                                                                                                         gAGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTc             <  1:174435/140‑1 (MQ=255)
                                                                                                                          aGCAGGCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTcg            <  1:331867/140‑1 (MQ=255)
                                                                                                                               gCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTa       >  2:898575/1‑140 (MQ=255)
                                                                                                                               gCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTa       >  2:1347088/1‑140 (MQ=255)
                                                                                                                               gCTTCTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTa       >  1:402959/1‑140 (MQ=255)
                                                                                                                                   cTGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAc   <  2:853996/140‑1 (MQ=255)
                                                                                                                                    tGGAAACCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAcc  <  1:669423/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GTTGCCATTGCGCCCAGTTTCATCATCGCTTTGATGACCTGAGAGCCTTTAACCGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACC  >  NC_000913/3314984‑3315255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A