Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F48 I1 R1	115	87.5	3003888	99.1%	2976853	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,567,589	C→A	A149S (GCG→TCG)	glgA ←	glycogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,567,589	0	C	A	98.0%	340.7 / ‑1.4	98	A149S (GCG→TCG)	glgA	glycogen synthase
Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (56/40); total (58/40)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 106 total aligned reads displayed.

GAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGCCGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTGTCGGTA  >  NC_000913/3567461‑3567725

gAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACg                                                                                                                               <  2:351743/140‑1 (MQ=255)

gAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGAGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACg                                                                                                                               <  1:547393/140‑1 (MQ=255)

       aGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGgcgc                                                                                                                        <  1:599792/140‑1 (MQ=255)

        gCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCa                                                                                                                       >  1:1456201/1‑140 (MQ=255)

          ccATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAg                                                                                                                     <  1:955991/140‑1 (MQ=255)

          ccATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAg                                                                                                                     <  1:945898/140‑1 (MQ=255)

           cATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAgg                                                                                                                    >  1:603441/1‑140 (MQ=255)

           cATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAgg                                                                                                                    >  2:44136/1‑140 (MQ=255)

             tGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGct                                                                                                                  >  2:580210/1‑139 (MQ=255)

              gAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCt                                                                                                                 >  1:525442/1‑140 (MQ=255)

                  atTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCAt                                                                                                             >  1:1212953/1‑140 (MQ=255)

                   ttAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATg                                                                                                            <  1:1091469/140‑1 (MQ=255)

                   ttAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATg                                                                                                            >  2:300101/1‑140 (MQ=255)

                     aaaGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGcc                                                                                                          >  1:207376/1‑140 (MQ=255)

                      aaGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCa                                                                                                         <  1:443419/140‑1 (MQ=255)

                           tGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGt                                                                                                    >  2:602958/1‑140 (MQ=255)

                           tGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGt                                                                                                    >  1:726452/1‑140 (MQ=255)

                           tGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGt                                                                                                    >  1:356539/1‑140 (MQ=255)

                            gACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTg                                                                                                   >  1:775843/1‑140 (MQ=255)

                            gACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTg                                                                                                   >  1:986524/1‑140 (MQ=255)

                             aCCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTgc                                                                                                  <  1:595302/140‑1 (MQ=255)

                                aTGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCa                                                                                               >  1:1296130/1‑140 (MQ=255)

                                aTGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCa                                                                                               <  1:1143489/140‑1 (MQ=255)

                                 tGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCAt                                                                                              >  1:307210/1‑140 (MQ=255)

                                  ggCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATg                                                                                             >  2:575456/1‑140 (MQ=255)

                                  ggCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATg                                                                                             <  2:726452/140‑1 (MQ=255)

                                       ttGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGcacca                                                                                        >  1:574042/1‑140 (MQ=255)

                                           aTGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATc                                                                                    >  1:267347/1‑140 (MQ=255)

                                            tGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCa                                                                                   >  2:344836/1‑140 (MQ=255)

                                               aattcatGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGa                                                                                <  2:18816/139‑1 (MQ=255)

                                                 ttcatGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACg                                                                              >  2:1367962/1‑140 (MQ=255)

                                                       tgatgtgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGTCAGTCGTGCGCATGCACCACATCAGGACGCCAGaa                                                                        <  2:1456201/140‑1 (MQ=255)

                                                       tgatgtgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGaa                                                                        <  1:344836/140‑1 (MQ=255)

                                                       tgatgtgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGaa                                                                        <  2:1296130/140‑1 (MQ=255)

                                                       tgatgtgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGaa                                                                        <  1:115703/140‑1 (MQ=255)

                                                        gatgtgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAAt                                                                       >  1:145454/1‑140 (MQ=255)

                                                          tgtgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGCCGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATgg                                                                     >  1:1271724/1‑140 (MQ=255)

                                                          tgtgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATgg                                                                     >  2:980021/1‑140 (MQ=255)

                                                           gtgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATggg                                                                    >  2:926196/1‑140 (MQ=255)

                                                            tgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGt                                                                   <  1:60545/140‑1 (MQ=255)

                                                            tgCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGt                                                                   >  1:1233057/1‑140 (MQ=255)

                                                            tgCATAAAACATGCCTTGATAGGCAAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGt                                                                   >  2:663491/1‑140 (MQ=255)

                                                              cATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCa                                                                 >  2:1341140/1‑140 (MQ=255)

                                                              cATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCa                                                                 >  1:1053044/1‑140 (MQ=255)

                                                              cATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCa                                                                 >  1:1430261/1‑140 (MQ=255)

                                                               aTAAAACATGCCTTGATAGGCCAGGTTGTGCTCAGTAAACACCGACTTCGCCGTACGCCCGCGCGCCGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGGCGCCAGAATGGGTCaa                                                                >  1:1228673/1‑140 (MQ=255)

                                                                    aCATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCg                                                           <  2:603441/140‑1 (MQ=255)

                                                                    aCATGCCTTGATAGCCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGACTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCg                                                           <  2:267347/140‑1 (MQ=255)

                                                                      aTGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCt                                                         >  1:476093/1‑140 (MQ=255)

                                                                        gCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTgg                                                       >  1:1133995/1‑140 (MQ=255)

                                                                        gCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTgg                                                       >  2:148983/1‑140 (MQ=255)

                                                                        gCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTgg                                                       >  2:1191618/1‑140 (MQ=255)

                                                                         ccTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGc                                                      <  2:1283015/140‑1 (MQ=255)

                                                                            tGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCAt                                                   <  2:1152642/140‑1 (MQ=255)

                                                                             gATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCAtt                                                  >  1:165298/1‑140 (MQ=255)

                                                                              aTAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCAttt                                                 <  1:1381602/140‑1 (MQ=255)

                                                                              aTAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCAttt                                                 >  2:507701/1‑140 (MQ=255)

                                                                              aTAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCAttt                                                 >  1:1322483/1‑140 (MQ=255)

                                                                               tAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGTGCAAGGCCTGCATGCCGGTCGTGCGCATGCACCACATCAGGGCGCCAGAATGGGTCAAGCCCGCTGGCCATTTc                                                <  1:580210/140‑1 (MQ=255)

                                                                                aGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCt                                               >  1:614954/1‑140 (MQ=255)

                                                                                  gCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGc                                             >  2:1452153/1‑140 (MQ=255)

                                                                                  gCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGc                                             >  1:1245137/1‑140 (MQ=255)

                                                                                  gCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGc                                             >  1:727993/1‑140 (MQ=255)

                                                                                   ccAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGcc                                            <  2:1430261/140‑1 (MQ=255)

                                                                                   ccAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGcc                                            >  2:664562/1‑140 (MQ=255)

                                                                                    cAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGccc                                           <  1:300101/140‑1 (MQ=255)

                                                                                       gTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCaa                                        >  1:522868/1‑140 (MQ=255)

                                                                                         tgtgCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAacc                                      <  2:1053044/140‑1 (MQ=255)

                                                                                           tgCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAaccca                                    >  2:1135851/1‑140 (MQ=255)

                                                                                            gCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAacccac                                   >  1:39621/1‑140 (MQ=255)

                                                                                             cacaGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAacccacc                                  >  1:288133/1‑140 (MQ=255)

                                                                                             cacaGTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAacccacc                                  >  2:1124587/1‑140 (MQ=255)

                                                                                                 gTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCcag                              <  1:1191618/140‑1 (MQ=255)

                                                                                                 gTAAACACCGACTTCGCCGGACGCCCGCGCGACGCCAGATAAGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCcag                              >  1:873541/1‑140 (MQ=255)

                                                                                                     acacCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCagcagc                          >  1:675685/1‑140 (MQ=255)

                                                                                                       acCGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAgcgc                        >  2:439631/1‑140 (MQ=255)

                                                                                                         cGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCaa                      <  1:602958/140‑1 (MQ=255)

                                                                                                         cGACTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCaa                      <  1:575456/140‑1 (MQ=255)

                                                                                                           aCTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCaaaa                    <  1:507701/140‑1 (MQ=255)

                                                                                                            cTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAAc                   >  2:804288/1‑140 (MQ=255)

                                                                                                            cTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAAc                   >  1:420783/1‑140 (MQ=255)

                                                                                                            cTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAAc                   >  2:1392548/1‑140 (MQ=255)

                                                                                                            cTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAAc                   >  2:539280/1‑140 (MQ=255)

                                                                                                            cTTCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCCGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAAc                   >  1:972325/1‑140 (MQ=255)

                                                                                                             ttCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACg                  <  1:1013764/140‑1 (MQ=255)

                                                                                                              tCGCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGc                 >  2:477316/1‑140 (MQ=255)

                                                                                                                gCCGGACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCaa               <  2:307210/140‑1 (MQ=255)

                                                                                                                   ggACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATAc            <  1:793415/140‑1 (MQ=255)

                                                                                                                   ggACGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATAc            >  1:1445628/1‑140 (MQ=255)

                                                                                                                     aCGCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGt          <  1:1341140/140‑1 (MQ=255)

                                                                                                                       gCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCTGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTg        <  2:775843/140‑1 (MQ=255)

                                                                                                                       gCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTg        <  2:439903/140‑1 (MQ=255)

                                                                                                                       gCCCGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTg        <  1:439631/140‑1 (MQ=255)

                                                                                                                        cccGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTGt       <  1:250983/140‑1 (MQ=255)

                                                                                                                        cccGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTGt       <  2:1445628/140‑1 (MQ=255)

                                                                                                                        cccGCGCGACGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTGt       <  2:614954/140‑1 (MQ=255)

                                                                                                                            cgcgaCGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTGTCGGt   <  1:663491/140‑1 (MQ=255)

                                                                                                                            cgcgaCGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTGTCGGt   <  1:1452153/140‑1 (MQ=255)

                                                                                                                             gcgaCGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCCCCCCAGCAGCGCAAAACGCAATACGTTGTCGGta  <  2:719184/140‑1 (MQ=255)

                                                                                                                             gcgaCGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTGTCGGta  <  1:148983/140‑1 (MQ=255)

GAATTCCAGCCCATGAATATTAAAGAATGACCATGGCAATTGGATGTCATTCATGTGATGTGCATAAAACATGCCTTGATAGGCCAGGTTGTGCACAGTAAACACCGACTTCGCCGGACGCCCGCGCGCCGCCAGATACGCAGGCGCAAGGCCTGCATGCCAGTCGTGCGCATGCACCACATCAGGACGCCAGAATGGGTCAAGCCCGCTGGCCATTTCTGCCCCAACCCACCCCAGCAGCGCAAAACGCAATACGTTGTCGGTA  >  NC_000913/3567461‑3567725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A