Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F48 I1 R1	115	87.5	3003888	99.1%	2976853	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,582,760	G→T	A300S (GCA→TCA)	yhhZ →	putative endonuclease YhhZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,582,760	0	G	T	99.5%	675.8 / ‑5.6	188	A300S (GCA→TCA)	yhhZ	putative endonuclease YhhZ
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (91/96); total (92/96)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 188 total aligned reads displayed.

TTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGAAAATTTAGTATTAATCGAAAA  >  NC_000913/3582629‑3582896

ttcttGATGGTATAGACATAATAGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGa                                                                                                                                  <  2:1309399/140‑1 (MQ=255)

          tataGACATAATAGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACg                                                                                                                        >  2:1253296/1‑140 (MQ=255)

          tataGACATAATAGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACg                                                                                                                        >  1:1085940/1‑140 (MQ=255)

            taGACATAATAGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGcc                                                                                                                      >  1:1025162/1‑140 (MQ=255)

                   aataGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGaa                                                                                                                   >  2:1066704/1‑136 (MQ=255)

                   aataGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGAcc                                                                                                               >  1:385443/1‑140 (MQ=255)

                    ataGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCt                                                                                                              >  1:8306/1‑140 (MQ=255)

                    ataGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCt                                                                                                              >  2:618838/1‑140 (MQ=255)

                    ataGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCt                                                                                                              >  1:637852/1‑140 (MQ=255)

                    ataGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCt                                                                                                              >  2:784233/1‑140 (MQ=255)

                    ataGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCt                                                                                                              >  1:1355825/1‑140 (MQ=255)

                    ataGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCt                                                                                                              >  2:1170942/1‑140 (MQ=255)

                      aGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCttt                                                                                                            >  2:159513/1‑140 (MQ=255)

                       gAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCtttt                                                                                                           <  2:1346771/140‑1 (MQ=255)

                          ataataTACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAg                                                                                                        >  2:511592/1‑140 (MQ=255)

                           taataTACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGa                                                                                                       >  1:470047/1‑140 (MQ=255)

                           taataTACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGa                                                                                                       >  2:260991/1‑140 (MQ=255)

                              tataCATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATg                                                                                                    >  1:867581/1‑140 (MQ=255)

                               ataCATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATga                                                                                                   >  2:1226683/1‑140 (MQ=255)

                                taCATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATgag                                                                                                  <  2:149805/140‑1 (MQ=255)

                                 aCATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATgaga                                                                                                 <  1:1479342/140‑1 (MQ=255)

                                 aCATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATgaga                                                                                                 <  1:618838/140‑1 (MQ=255)

                                 aCATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATgaga                                                                                                 <  2:1355825/140‑1 (MQ=255)

                                   aTTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGaaa                                                                                               >  1:1437319/1‑140 (MQ=255)

                                     tAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAgg                                                                                             >  1:561578/1‑140 (MQ=255)

                                     tAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAAGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAgg                                                                                             >  2:1422709/1‑140 (MQ=255)

                                      aaGAGACCTTCAAAATACTTAGCTAACTTACTAAATGAATTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACATGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAggg                                                                                            <  1:212357/140‑1 (MQ=255)

                                       agagACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGc                                                                                           >  1:165557/1‑140 (MQ=255)

                                       agagACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGc                                                                                           >  1:662732/1‑140 (MQ=255)

                                           aCCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATg                                                                                       <  1:916763/140‑1 (MQ=255)

                                           aCCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATg                                                                                       >  2:1028803/1‑140 (MQ=255)

                                             cTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAGGACCTTTTAAGAATGAGAAAGGGCAATGtt                                                                                     >  1:119732/1‑140 (MQ=255)

                                             cTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGtt                                                                                     >  1:331429/1‑140 (MQ=255)

                                             cTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGtt                                                                                     >  2:1461642/1‑140 (MQ=255)

                                             cTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGtt                                                                                     >  1:7548/1‑140 (MQ=255)

                                             cTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGtt                                                                                     >  1:1215094/1‑140 (MQ=255)

                                             cTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGtt                                                                                     >  2:702300/1‑140 (MQ=255)

                                             cTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGtt                                                                                     >  2:734499/1‑140 (MQ=255)

                                             cTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGtt                                                                                     >  2:1390543/1‑140 (MQ=255)

                                             cTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGtt                                                                                     >  1:1410229/1‑140 (MQ=255)

                                               tCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTct                                                                                   >  2:804076/1‑139 (MQ=255)

                                               tCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTcc                                                                                   >  1:1224330/1‑140 (MQ=255)

                                               tCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTcc                                                                                   >  1:626910/1‑140 (MQ=255)

                                               tCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGCAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTcc                                                                                   >  2:407425/1‑140 (MQ=255)

                                                cAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCct                                                                                  >  2:582402/1‑140 (MQ=255)

                                                cAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCct                                                                                  <  1:702300/140‑1 (MQ=255)

                                                cAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCct                                                                                  <  1:1257124/140‑1 (MQ=255)

                                                cAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAACGAGAAAGGGCAATGTTCct                                                                                  <  2:949089/140‑1 (MQ=255)

                                                cAAAAGACTTAGCTAACTTACGAAAGGAATTTAATAAACCTGTAAGAAACAATTTTCTTATCAAACATGAACACACCCCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGACTGTGAAAGGGCACTGTTCct                                                                                  <  2:1410229/140‑1 (MQ=255)

                                                  aaaGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCctct                                                                                <  1:1170942/140‑1 (MQ=255)

                                                  aaaGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCctct                                                                                <  1:1312678/140‑1 (MQ=255)

                                                       cTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAAtt                                                                           >  2:220775/1‑140 (MQ=255)

                                                       cTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAAtt                                                                           <  1:98493/140‑1 (MQ=255)

                                                       cTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAAtt                                                                           >  2:921117/1‑140 (MQ=255)

                                                       cTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAAtt                                                                           <  1:1242215/140‑1 (MQ=255)

                                                        ttAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATta                                                                          <  1:1461642/140‑1 (MQ=255)

                                                        ttAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATta                                                                          >  1:992315/1‑140 (MQ=255)

                                                         tAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATtat                                                                         >  1:373195/1‑140 (MQ=255)

                                                         tAGCTAACTTACGAAAGGAGCTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATtat                                                                         >  1:1375743/1‑140 (MQ=255)

                                                          aGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATtata                                                                        >  1:786033/1‑140 (MQ=255)

                                                          aGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATtata                                                                        <  2:75987/140‑1 (MQ=255)

                                                              aaCTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGt                                                                    <  2:119732/140‑1 (MQ=255)

                                                              aaCTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGt                                                                    <  2:197335/140‑1 (MQ=255)

                                                              aaCTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGt                                                                    <  1:784233/140‑1 (MQ=255)

                                                              aaCTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGt                                                                    <  1:740268/140‑1 (MQ=255)

                                                               aCTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGtt                                                                   <  1:804076/140‑1 (MQ=255)

                                                               aCTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGtt                                                                   <  2:907722/140‑1 (MQ=255)

                                                               aCTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGtt                                                                   <  1:875933/140‑1 (MQ=255)

                                                                cTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTc                                                                  <  2:867581/140‑1 (MQ=255)

                                                                   aCGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCAcc                                                               >  2:256110/1‑140 (MQ=255)

                                                                    cGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCa                                                              >  2:1280786/1‑140 (MQ=255)

                                                                    cGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCa                                                              <  1:798203/140‑1 (MQ=255)

                                                                        aGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATaaa                                                          <  1:1028803/140‑1 (MQ=255)

                                                                        aGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATaaa                                                          <  2:801588/140‑1 (MQ=255)

                                                                         ggAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAAc                                                         >  1:417978/1‑140 (MQ=255)

                                                                          gAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACt                                                        <  2:786033/140‑1 (MQ=255)

                                                                          gAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACt                                                        <  2:653596/140‑1 (MQ=255)

                                                                          gAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACt                                                        <  2:752947/140‑1 (MQ=255)

                                                                           aGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTa                                                       >  1:210509/1‑140 (MQ=255)

                                                                              ttAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTAtct                                                    <  1:1280786/140‑1 (MQ=255)

                                                                                   aaaaCTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAga                                               <  2:361485/140‑1 (MQ=255)

                                                                                       cTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAgatgat                                           >  1:309830/1‑140 (MQ=255)

                                                                                         gTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGAtgg                                         <  2:1224330/140‑1 (MQ=255)

                                                                                         gTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGAtgg                                         <  2:385443/140‑1 (MQ=255)

                                                                                         gTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGAtgg                                         <  2:1003903/140‑1 (MQ=255)

                                                                                          tAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGAtggt                                        >  2:980276/1‑140 (MQ=255)

                                                                                          tAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGAtggt                                        >  1:1064187/1‑140 (MQ=255)

                                                                                          tAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGAtggt                                        >  2:1086435/1‑140 (MQ=255)

                                                                                          tAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGAtggt                                        <  2:31888/140‑1 (MQ=255)

                                                                                           aaGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGAtggtg                                       <  2:828182/140‑1 (MQ=255)

                                                                                            aGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGAtgg                                         <  1:962770/137‑1 (MQ=255)

                                                                                               aaaaaTTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTAc                                   >  2:175368/1‑140 (MQ=255)

                                                                                                   aTTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGGCCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAAt                               <  1:407425/140‑1 (MQ=255)

                                                                                                   aTTTTCTTATCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAAt                               <  1:235749/140‑1 (MQ=255)

                                                                                                           aTCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGa                       <  1:511592/140‑1 (MQ=255)

                                                                                                           aTCAAACTTGCAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGa                       <  2:46205/140‑1 (MQ=255)

                                                                                                                     cAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGAAAATTTAGTa             <  1:582402/140‑1 (MQ=255)

                                                                                                                     cAAAAACCTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGAAAATTTAGTa             >  2:612400/1‑140 (MQ=255)

                                                                                                                           ccTCCGAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGAAAATTTAGTATTAATc       <  1:941355/140‑1 (MQ=255)

                                                                                                                                gAATCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGAAAATTTAGTATTAATCGaaaa  <  1:1179173/140‑1 (MQ=255)

TTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGAAAATTTAGTATTAATCGAAAA  >  NC_000913/3582629‑3582896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A