Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F48 I1 R1	82	50.4	1709896	99.0%	1692797	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,585,618	G→T	N402K (AAC→AAA)	ggt ←	glutathione hydrolase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,585,618	0	G	T	100.0%	192.1 / NA	54	N402K (AAC→AAA)	ggt	glutathione hydrolase proenzyme
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (23/31); total (23/31)

CCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGCATA  >  NC_000913/3585482‑3585749

ccaccaGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTa                                                                                                                                  >  2:650624/1‑140 (MQ=255)

ccaccaGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTa                                                                                                                                  >  1:239490/1‑140 (MQ=255)

 caccaGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTAc                                                                                                                                 <  2:371458/140‑1 (MQ=255)

      gCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCt                                                                                                                            <  2:231227/140‑1 (MQ=255)

          gTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTAt                                                                                                                        >  1:9388/1‑140 (MQ=255)

                 ttCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCcaccac                                                                                                                 >  2:602654/1‑140 (MQ=255)

                  tCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACt                                                                                                                <  2:73505/140‑1 (MQ=255)

                  tCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACt                                                                                                                <  1:650624/140‑1 (MQ=255)

                  tCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACt                                                                                                                <  2:51522/140‑1 (MQ=255)

                  tCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACt                                                                                                                >  1:662024/1‑140 (MQ=255)

                  tCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACt                                                                                                                <  1:292502/140‑1 (MQ=255)

                  tCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCACCACCGCTTTACCATCTTTATCCACCACt                                                                                                                <  1:834353/140‑1 (MQ=255)

                    ggTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGa                                                                                                              <  1:684106/140‑1 (MQ=255)

                     gTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAg                                                                                                             >  1:783592/1‑140 (MQ=255)

                       aCGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTa                                                                                                           <  2:549845/140‑1 (MQ=255)

                         gCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAAt                                                                                                         <  2:499696/140‑1 (MQ=255)

                          cccGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATg                                                                                                        <  1:184381/140‑1 (MQ=255)

                            cGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATggg                                                                                                      <  1:658136/140‑1 (MQ=255)

                               tttGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGTTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAg                                                                                                   <  2:748436/140‑1 (MQ=255)

                                     ggAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGAt                                                                                             <  1:368940/140‑1 (MQ=255)

                                     ggAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGAt                                                                                             <  2:239490/140‑1 (MQ=255)

                                       agaAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTa                                                                                           <  2:9388/140‑1 (MQ=255)

                                                   tcTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGgc                                                                               >  1:357707/1‑140 (MQ=255)

                                                     tGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGgcgc                                                                             >  1:281353/1‑140 (MQ=255)

                                                        ttattaAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAg                                                                          >  1:233435/1‑140 (MQ=255)

                                                                  gAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCgggg                                                                >  1:118510/1‑140 (MQ=255)

                                                                   aaTACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGc                                                               >  2:12194/1‑140 (MQ=255)

                                                                          ctctCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTc                                                        >  1:279704/1‑140 (MQ=255)

                                                                          ctctCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTc                                                        >  2:358879/1‑140 (MQ=255)

                                                                             tcGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCt                                                     >  1:238411/1‑140 (MQ=255)

                                                                             tcGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCt                                                     >  1:523238/1‑140 (MQ=255)

                                                                             tcGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCt                                                     >  1:473225/1‑140 (MQ=255)

                                                                               gCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTgg                                                   >  1:158613/1‑140 (MQ=255)

                                                                                cccGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGa                                                  <  1:89375/140‑1 (MQ=255)

                                                                                cccGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGACAACGCTTTACCATCTTTATCCACCACAGTGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGa                                                  <  2:231989/140‑1 (MQ=255)

                                                                                    cgACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGc                                              <  1:12194/140‑1 (MQ=255)

                                                                                     gACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCt                                             <  2:473225/140‑1 (MQ=255)

                                                                                     gACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCt                                             <  2:662024/140‑1 (MQ=255)

                                                                                     gACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCt                                             <  2:281353/140‑1 (MQ=255)

                                                                                     gACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCt                                             <  2:279704/140‑1 (MQ=255)

                                                                                     gACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCt                                             <  2:194627/140‑1 (MQ=255)

                                                                                                    gAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGatat                              >  2:86048/1‑140 (MQ=255)

                                                                                                           gtgtTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAAtttt                       >  2:744636/1‑139 (MQ=255)

                                                                                                            tgtTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGa                      >  1:111172/1‑140 (MQ=255)

                                                                                                             gtTCAGCGTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGAt                     <  2:111172/140‑1 (MQ=255)

                                                                                                                    gTATAGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAAt              <  1:602654/140‑1 (MQ=255)

                                                                                                                     tataGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATa             >  1:502776/1‑140 (MQ=255)

                                                                                                                        aGGTCACCGCCACCGCTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGAt          <  2:238411/140‑1 (MQ=255)

                                                                                                                             accgccaccgcTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGc     <  2:493405/140‑1 (MQ=255)

                                                                                                                             accgccaccgcTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGc     >  1:37342/1‑140 (MQ=255)

                                                                                                                             accgccaccgcTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGc     >  2:751789/1‑140 (MQ=255)

                                                                                                                              ccgccaccgcTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGCa    <  1:265110/140‑1 (MQ=255)

                                                                                                                               cgccaccgcTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGCAt   <  1:543088/140‑1 (MQ=255)

                                                                                                                                gccaccgcTTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGCATa  <  1:86048/140‑1 (MQ=255)

CCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTCTCATAAGGCGCAAGCTTGCCGGGGCGAATTTCGCTGGATGGCTTCGCTTTATTGATATCAATTTGATCGGCAATAGATTTGGCATA  >  NC_000913/3585482‑3585749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A