Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F48 I1 R1	115	87.5	3003888	99.1%	2976853	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,042,058	G→T	intergenic (‑59/‑11)	mobA ← / → yihD	molybdenum cofactor guanylyltransferase/conserved protein YihD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,042,058	0	G	T	100.0%	193.3 / NA	54	intergenic (‑59/‑11)	mobA/yihD	molybdenum cofactor guanylyltransferase/conserved protein YihD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (27/27); total (27/27)

TCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACT  >  NC_000913/4041922‑4042193

tCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAg                                                                                                                                      >  1:1203541/1‑140 (MQ=255)

tCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAg                                                                                                                                      <  1:462649/140‑1 (MQ=255)

tCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCACCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAg                                                                                                                                      <  1:242143/140‑1 (MQ=255)

  cTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCa                                                                                                                                    <  1:1056715/140‑1 (MQ=255)

  cTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCa                                                                                                                                    <  1:1226436/140‑1 (MQ=255)

  cTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCa                                                                                                                                    <  2:1466077/140‑1 (MQ=255)

    ttATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATc                                                                                                                                  >  1:1178351/1‑140 (MQ=255)

     tATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCa                                                                                                                                 >  1:552367/1‑140 (MQ=255)

      aTCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCAc                                                                                                                                >  2:505204/1‑140 (MQ=255)

          aCGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATg                                                                                                                            <  1:1120186/140‑1 (MQ=255)

          aCGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATg                                                                                                                            <  2:666984/140‑1 (MQ=255)

          aCGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATg                                                                                                                            <  1:1274255/140‑1 (MQ=255)

          aCGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAACACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATg                                                                                                                            <  1:507114/140‑1 (MQ=255)

           cgccgcCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGa                                                                                                                           >  2:839079/1‑140 (MQ=255)

           cgccgcCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGa                                                                                                                           >  1:726338/1‑140 (MQ=255)

            gccgccCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGaa                                                                                                                          >  2:1348329/1‑140 (MQ=255)

                 ccATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTa                                                                                                                     <  1:184831/140‑1 (MQ=255)

                 ccATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTa                                                                                                                     >  1:305095/1‑140 (MQ=255)

                      cGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGt                                                                                                                <  1:506171/140‑1 (MQ=255)

                      cGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGt                                                                                                                <  2:305095/140‑1 (MQ=255)

                             cTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCtgaatg                                                                                                         <  2:658132/140‑1 (MQ=255)

                                tACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAg                                                                                                      <  2:1086227/140‑1 (MQ=255)

                                 aCCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGt                                                                                                     <  1:839079/140‑1 (MQ=255)

                                    gCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTAt                                                                                                  <  1:1394981/140‑1 (MQ=255)

                                    gCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTAt                                                                                                  <  2:202688/140‑1 (MQ=255)

                                        gCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGaa                                                                                              <  2:1432043/140‑1 (MQ=255)

                                        gCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGaa                                                                                              <  1:1219947/140‑1 (MQ=255)

                                        gCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGaa                                                                                              <  1:693021/140‑1 (MQ=255)

                                                  cGCCTGTTATCTTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCGTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTccagc                                                                                    >  1:1261959/1‑140 (MQ=255)

                                                  cGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAACCGTCTGAATGAAGTTATTGAACTCCTccagc                                                                                    >  1:122976/1‑140 (MQ=255)

                                                              tcgtcATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCaaa                                                                        >  1:761931/1‑140 (MQ=255)

                                                              tcgtcATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCaaa                                                                        <  2:53594/140‑1 (MQ=255)

                                                                gtcATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCAAGCCAGCCTGGCaaaaa                                                                      <  1:437585/140‑1 (MQ=255)

                                                                     caGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGcc                                                                 <  2:552367/140‑1 (MQ=255)

                                                                             ccGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTa                                                         >  2:1255398/1‑140 (MQ=255)

                                                                             ccGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTa                                                         <  1:1348329/140‑1 (MQ=255)

                                                                                  atCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACctg                                                    >  2:360955/1‑140 (MQ=255)

                                                                                          ttttATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAAtt                                            >  1:1161739/1‑140 (MQ=255)

                                                                                          ttttATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAAtt                                            <  2:1261959/140‑1 (MQ=255)

                                                                                                 gtgGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAg                                     <  2:122976/140‑1 (MQ=255)

                                                                                                    ggATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGaat                                  >  2:1195944/1‑139 (MQ=255)

                                                                                                    ggATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGaaa                                  >  1:209162/1‑140 (MQ=255)

                                                                                                    ggATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGaaa                                  >  2:1327528/1‑140 (MQ=255)

                                                                                                    ggATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGaaa                                  >  2:727399/1‑140 (MQ=255)

                                                                                                    ggATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGaaa                                  >  1:1204630/1‑140 (MQ=255)

                                                                                                    ggATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGaaa                                  >  1:56766/1‑140 (MQ=255)

                                                                                                    ggATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGaaa                                  >  1:797499/1‑140 (MQ=255)

                                                                                                    ggATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGaaa                                  >  2:471434/1‑140 (MQ=255)

                                                                                                           cccTGCTAACGTGTCTGTCTCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGa                           <  2:797499/140‑1 (MQ=255)

                                                                                                                        tctgtctCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCACCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGttt              >  1:615266/1‑140 (MQ=255)

                                                                                                                          tgtctCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGttttt            >  2:34653/1‑139 (MQ=255)

                                                                                                                          tgtctCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTg            >  1:1499066/1‑140 (MQ=255)

                                                                                                                          tgtctCAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTACAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTg            >  2:801446/1‑140 (MQ=255)

                                                                                                                               cAAGAGTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGc       >  2:347087/1‑140 (MQ=255)

                                                                                                                                    gTAATGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACt  >  2:435471/1‑140 (MQ=255)

TCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACT  >  NC_000913/4041922‑4042193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A